DOI QR코드

DOI QR Code

Isolation of Lipase Producing Bacillus subtilis and Some Characteristics of the Enzyme

중성 Lipase를 생산하는 Bacillus subtilis JKA-3의 분리 동정 및 효소 특성

  • Jo, Ji-Won (Graduate School of Molecular & Biomedical Technology, Inje University) ;
  • Hur, Sung-Ho (Department of Clinical Laboratory Science, Dong-eui Institute of Technology) ;
  • Han, Yong-Soo (Department of Cosmetology, Gwangyang Health College) ;
  • Kim, Ji-Yeon (Graduate School of Molecular & Biomedical Technology, College of General Education, Inje University)
  • 조지원 (인제대학교 대학원 분자의 생명공학과) ;
  • 허성호 (동의과학대학 임상병리과) ;
  • 한용수 (광양보건대학 피부미용과) ;
  • 김지연 (인제대학교 대학원 분자의 생명공학과, 기초대학)
  • Published : 2009.09.30

Abstract

As part of an investigation to identify microorganisms that are biotechnologically interesting for industrial application, we isolated a bacterial strain from Chungkookjang that produces extracellular neutral lipase. In addition, the crude enzyme was characterized. This isolated strain, designated as JKA-3 was identified as Bacillus subtilis JKA-3 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The cells were rod-shaped and $0.6-0.8{\times}2.0-2.3\;{\mu}m$ in size. Optimal growth conditions were $35-40^{\circ}C$ and pH 6.0-8.0. The isolate was able to grow in up to 0-10.0% (w/v) NaCl. Optimal activity conditions of the crude lipase fraction of B. subtilis JKA-3 were pH of 7.0 at $35^{\circ}C$. This enzyme was stable in the pH ranging 6.0-8.0.

Keywords

References

  1. Altschul SF, Madden TL, Schffer AA, Zhang J, Zhang Z, Miller W, and Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389-3402 https://doi.org/10.1093/nar/25.17.3389
  2. Claus D and Berkeley RCW (1986) Genus Bacillus. In Bergey's Manual of Systematic Bacteriology, pp. 1105-1139, Williams & Wilkins, Baltimore, MD, U.S.A.
  3. Demirjian DC, Francisco MV, and Cassidy CS (2001) Enzymes from extremopiles. Curr Opin Biotechnol 5, 144-151 https://doi.org/10.1016/S1367-5931(00)00183-6
  4. Diaz P, Prim N, and Pastor FIJ (1999) Direct fluorescence based lipase activity assay. Biotechniques 27, 697-699
  5. Erturula S, Dnmeza q and Takab S (2007) Isolation of lipase producing Bacillus sp. from olive mill wastewater and improving its enzyme activity. J Hazard Mater 149, 720-724 https://doi.org/10.1016/j.jhazmat.2007.04.034
  6. Jaeger KE and Reetz MT (1998) Microbial lipases from versatile tools for biotechnology. Trends Biotechnol 16, 396-403 https://doi.org/10.1016/S0167-7799(98)01195-0
  7. Jaeger KE and Eggert T (2002) Lipase for biotechnology. Curr Opin Biotechnol 13, 390-397 https://doi.org/10.1016/S0958-1669(02)00341-5
  8. Jaeger KE, Ransac S, Dijkstra BW, Colson C, Heuvel M, and Misset O (1994) Bacterial lipases. FEMS Microbiol Rev 15, 29-63 https://doi.org/10.1111/j.1574-6976.1994.tb00121.x
  9. Khyami-Horani H (1996) Therrnotolerant strain of Bacillus licheniformis producing lipase. World J Microbiol Biotechnol 12, 399-401 https://doi.org/10.1007/BF00340219
  10. Lin SF, Chiou eM, Yeh CM, and Tsai YC (1996) Purification and partial characterization of an alkaline lipase from Pseudomonas pseudoalcaligenes F-111. Appl Environ Microbiol 62, 1093-1095
  11. Meghwanshi GK, Agarwal L, Dutt K, and Saxena RK (2006) Characterization of 1,3-regiospecific lipases from new Pseudomonas and Bacillus isolates. J Mol Catal B Enzym 40, 127-131 https://doi.org/10.1016/j.molcatb.2006.02.020
  12. Park MH, Ryu HJ, and Oh KK (2004) Isolation of lipase producing yeast and optimization of cultivation condition. Korean J Biotechnol Bioeng 19, 148-153
  13. Reetz MT (2002) Lipases as practical biocatalysts. Curr Opin Chem BioI 6, 145-150 https://doi.org/10.1016/S1367-5931(02)00297-1
  14. Ruiz C, Pastor FIJ, and Diaz P (2002) Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase. FEMS Microbiol Lett 217, 263-267 https://doi.org/10.1111/j.1574-6968.2002.tb11485.x
  15. Saitou Nand Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol BioI Evol 4, 406-425
  16. Schmid A, Dordick JS, Hauer B, Kiner A, Wubbolts M, and Witholt B (2001) Industrial biocatalysis today and tomorrow. Nature 409, 258-268 https://doi.org/10.1038/35051736
  17. Schmidt-Dannert C (1999) Recombinant microbial lipase for biotechnological applications. Bioorg Med Chem 7, 2123-2130 https://doi.org/10.1016/S0968-0896(99)00141-8
  18. Thompson, JD, Gibson TJ, Plewniak F, Jeanmougin F, and Higgins DG (1997) The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876-4882 https://doi.org/10.1093/nar/25.24.4876
  19. Weisburg WG, Barns SM, Pelletier A, and Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697-703 https://doi.org/10.1128/jb.173.2.697-703.1991

Cited by

  1. Antiobesity Effect of the Bacillus subtilis KC-3 Fermented Soymilk in 3T3-L1 Adipocytes vol.39, pp.8, 2010, https://doi.org/10.3746/jkfn.2010.39.8.1126
  2. 메주로부터 지질분해 효소 생산 균주의 분리 및 배양학적 특성 vol.40, pp.2, 2012, https://doi.org/10.4014/kjmb.1205.05010