Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Inhibition on LDL-oxidation by Phenolic Compounds from the Fruit Body of Phellinus linteus

  • Lyu, Ha-Na (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Dae-Young (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Min-Kyung (Graduate School of Biotechnology, Kyung Hee University) ;
  • Cho, Moon-Hee (National Research Laboratory of Lipid Metabolism & Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeong, Tae-Sook (National Research Laboratory of Lipid Metabolism & Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, In-Ho (Korea Food Research Institute) ;
  • Lee, Chang-Ho (Korea Food Research Institute) ;
  • Baek, Nam-In (Graduate School of Biotechnology, Kyung Hee University)
  • Published : 2009.09.30
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Abstract

The fruit body of Phellinus linteus was extracted with 80% aqueous MeOH, and the concentrated extract was successively partitioned using EtOAc, n-BuOH and $H_2O$. From the EtOAc and n-BuOH fractions, three phenolic compounds were isolated through repeated silica gel and ODS column chromatography. The chemical structures of these compounds were determined as 2-(3',4'-dihydroxyphenyl)-1,3-benzodioxole-5-aldehyde (1), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (2), and protocatechuic acid methyl ester (3) by spectroscopic data including NMR, MS and IR. Compounds $1{\sim}3$ exhibited low density lipoprotein (LDL) antioxidant activity with $IC_{50}$ values of 1.7, 0.7, and $2.4{\mu}M$, respectively.

Keywords

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