Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effects of Phytophthora Blight-antagonistic Microorganisms Bacillus subtilis AH18 and Bacillus licheniformis K11 on the Soil Microbial Community

고추역병 길항미생물 Bacillus subtilis AH18과 Bacillus licheniformis K11의 토양미생물 생태에 미치는 영향

  • Park, Kee-Choon (Ginseng Research Division, Rural Development Administration) ;
  • Lim, Jong-Hui (Department of Applied Microbiology, College of Natural Resources, Yeungnam University) ;
  • Kim, Sang-Dal (Department of Applied Microbiology, College of Natural Resources, Yeungnam University) ;
  • Yi, Young-Keun (School of Bioresource Science, Andong National University)
  • 박기춘 (농촌진흥청 인삼과) ;
  • 임종희 (영남대학교 응용미생물학과) ;
  • 김상달 (영남대학교 응용미생물학과) ;
  • 이영근 (안동대학교 식물의학과)
  • Published : 2009.09.30
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Abstract

We measured the influence of antifungal antagonists Bacillus subtilis AH18 and Bacillus licheniformis K11 on soil microbial community in microcosms. Both antifungal antagonists were confirmed to suppress hot pepper phytophthora blight. Phospholipid fatty acids (PLFA) were analyzed to investigate the soil microbial community. B. subtilis AH18 changed the total PLFA composition and bio-indicators of PLFA, compared with other treatments. B. subtilis AH18 decreased the proportion of bacteria and gram negative/gram positive bacteria, and increased the fungi/bacteria and anaerobic/aerobic microorganisms. In addition cy19:0/18:$1{\omega}7c$, which means adaptation to unfavorable environmental conditions, was increased by the application of B. subtilis AH18. On the other hand the inoculation of B. licheniformis K11 or combined inoculation of both antifungal strains did not affect soil microbial community. The suppression of phytophthora blight and preservation of indigenous soil microbial community may be achieved by the combined application of B. subtilis AH18 and B. licheniformis K11.

고추역병에 대한 길항미생물로 선발된 Bacillus subtilis AH18과 Bacillus licheniformis K11의 토양미생물 생태에 미치는 영향을 조사하기 위하여 이들 미생물을 단독 또는 복합으로 토양에 처리한 후 토양미생물상의 변화를 관찰하였다. 토양미생물 생태의 변화는 토양에서 인지질 지방산을 추출한 후 전체 지방산과 지표 지방산의 상대적 비율로 분석하였다. 전체적 인지질 지방산 구성과 각 지표 지방산 분석에 의해서 B. subtilis AH18 처리구는 무처리 대조구와 완전히 구분되었다. 전체 토양미생물상을 B. subtilis AH18 처리가 바꾸었고 세균 비율을 감소시켰으며, 곰팡이세균 비율을 높였으며, 그램음성균/그램양성균의 비율을 낮추었으며, 혐기성균/호기성균의 비율은 증가시켰다. 또한 불량환경 적응성을 의미하는 cy19:0/18:$1{\omega}7c$ 지방산비율과 단불포화지방산/포화지방산 비율도 B. subtilis AH18 처리구가 증가시켰다. 이에 반하여 B. licheniformis K11 처리나 두 미생물의 동시 처리는 전술한 토양미생물 지표에 큰 영향을 미치지 않았다. 따라서 지방산 분석을 토양미생물상 분석에서 B. subtilis AH18의 단용처리는 미생물을 처리하지 않은 대조구에 비하여 토양미생물상을 뚜렷이 바꾸었지만, B. subtilis AH18가 B. licheniformis K11와 혼용되었을 때는 토양미생물상 변화에 미치는 영향이 적었다. 따라서 이들 두 미생물을 동시에 처리할 경우 고추역병 방제와 토양미생물 생태 보존의 두 목적을 동시에 이룰 수 있을 것으로 기대된다.

Keywords

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