The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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DHA and EPA Down-regulate COX-2 Expression through Suppression of $NF-{\kappa}B$ Activity in LPS-treated Human Umbilical Vein Endothelial Cells

  • Lee, Soon-Ae (Department of Obstetrics & Gynecology, Gyeongsang National University) ;
  • Kim, Hye-Jung (Institute of Health Sciences, Gyeongsang National University) ;
  • Chang, Ki-Churl (Institute of Health Sciences, Gyeongsang National University) ;
  • Baek, Jong-Chul (Department of Obstetrics & Gynecology, Gyeongsang National University) ;
  • Park, Ji-Kwon (Department of Obstetrics & Gynecology, Gyeongsang National University) ;
  • Shin, Jeong-Kyu (Department of Obstetrics & Gynecology, Gyeongsang National University) ;
  • Choi, Won-Jun (Department of Obstetrics & Gynecology, Gyeongsang National University) ;
  • Lee, Jong-Hak (Department of Obstetrics & Gynecology, Gyeongsang National University) ;
  • Paik, Won-Young (Department of Obstetrics & Gynecology, Gyeongsang National University)
  • Published : 2009.08.31
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Abstract

Inflammatory processes of vascular endothelial cells play a key role in the development ofatherosclerosis. We determined the anti-inflammatory effects and mechanisms of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on LPS-treated human umbilical vein endothelial cells (HUVECs) to evaluate their cardioproteerive potential. Cells were pretreated with DHA, EPA, or troglitazone prior to activation with LPS. Expression of COX-2, prostaglandin $E_2$ ($PGE_2$) and IL-6 production, and $NF-{\kappa}B$ activity were measured by Western blot, ELISA, and luciferase activity, respectively. Results showed that EPA, DHA, or troglitazone significantly reduced COX-2 expression, $NF-{\kappa}B$ luciferase activity, and $PGE_2$ and IL-6 production in a dose-dependent fashion. Interestingly, low doses (10 ${\mu}$M) of DHA and EPA, but not troglitozone, significantly increased the activity of $NF-{\kappa}B$ in resting HUVECs. Our study suggests that while DHA, EPA, and troglitazone may be protective on HUVECs under inflammatory conditions in a dose-dependent manner. However there may be some negative effects when the concentrations are abnormally low, even in normal endothelium.

Keywords

References

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