한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제22권2호
  • /
  • Pages.19-38
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  • 2009
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
권호 표지

LPS로 활성화된 복강 대식세포에서 마황(麻黃)이 배오(配伍)된 10종(種) 처방의 NO 억제 효과

Inhibitory Effect of Ten Kinds of Herbal Medicine Including Ephedrae Herba on Nitric Oxide in LPS-induced Murine Peritoneal Macrophage

  • 배주연 (원광대학교 한의과대학 안이비인후피부과) ;
  • 조은희 (원광대학교 한의과대학 침구과) ;
  • 김훈영 (원광대학교 운영 군산의료원 산부인과) ;
  • 박민철 (원광대학교 한의과대학 안이비인후피부과)
  • 발행 : 2009.08.25
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초록

Objective : The purpose of this study is to find out whether Herba Ephedrae, solely used, and Herbal medicine in which this was included, have inhibitory effects of Nitric Oxide(NO). Methods : We tested the inhibitory effects of Nitric Oxide(NO) with Herba Ephedrae and ten kinds of Herbal medicine combined with Herba Ephedrae(Worlbikachul-Tang, 越婢加朮湯; Mahaengkamsuk-Tang, 麻杏甘石湯; Shinbi-Tang, 神秘湯; Mahwangbujaseshin-Tang, 麻黃附子細辛湯; Euiin-Tang, 薏苡仁湯; Galgeun-Tang, 葛根湯; Mahaengeuigam-Tang, 麻杏薏甘湯; Mahwang-Tang, 麻黃湯; Socheongryong-Tang, 小靑龍湯; Gaemagakban-Tang, 桂麻各半湯) on RAW264.7 cells. Results and Conclusions : 1. We carried out MTT assay on Herba Ephedrae and those decoctions including this in order to determine whether they accommodate cotytoxicity. The results were that Worlbikachul-Tang, Mahaengkamsuk-Tang, Mahwangbujaseshin-Tang, Mahaengeuigam-Tang, Mahwang-Tang, Socheongryong-Tang and Gaemagakban-Tang showed no cytotoxicity on RAW264.7 with 0.1mg/ml and 0.5mg/ml dosages of decoctions but displayed cytotoxicity on the cell with 1mg/ml. Solely used Herba Ephedrae, Shinbi-Tang, Euiin-Tang and Galgeun-Tang exhibited cytotoxicity beyond the concentration of 0.5mg/ml. 2. Worlbikachul-Tang, Mahaengkamsuk-Tang, Shinbi-Tang, Mahwangbujaseshin-Tang, Euiin-Tang, Galgeun-Tang, Mahaengeuigam-Tang, Mahwang-Tang and Socheongryong-Tang showed inhibition of NO production but solely used Herba Ephedrae and Gaemagakban-Tang did not exhibit such reaction.

키워드

참고문헌

  1. Kuo, M. L. Chau, Y. P., Wang, J. H. and Shiah, S. G.. Inhibitors of poly(ADP-ribose) polymerase block nitric oxide-induced apoptosis but not differentiation in human leukemia HL-60 cells. Biochem. Biophys. Res. Commun.. 1996;219:502-8. https://doi.org/10.1006/bbrc.1996.0263
  2. Sessa WC. The nitric oxide synthase family of proteins. J Vasc Res. 1994;31:131-43. https://doi.org/10.1159/000159039
  3. Nathan C, Xie QW. Regulation of biosynthesis of nitric oxide. J Biol Chem.1994;269:13725-8.
  4. Wimalawansa SJ. Nitric oxide: new evidence for novel therapeutic indications.Expert Opin Pharmacother. 2008 Aug;9(11):1935-54. https://doi.org/10.1517/14656566.9.11.1935
  5. Matsuda H, Morikawa T, Ando S, Toguchida I and Yoshikawa M. Structuralrequirements of flavonoids for nitric oxide production inhibitory activity andmechanism of action. Bioorganic Med. Chem. 11. 1995-2000(2003). https://doi.org/10.1016/S0968-0896(03)00067-1
  6. Calixto JB, Campos MM, Otuki MF, Santos AR:Anti-inflammatory compoundsof plant origin. Part II. modulation of pro-inflammatory cytokines, chemokinesand adhesion molecules. Planta Med. 2004;70(2):93-103. https://doi.org/10.1055/s-2004-815483
  7. 신민교. 臨床本草學. 영림사. 2002:322-3.
  8. 김동현, 김형민, 류종훈, 엄재영, 김상찬, 양재하, 조민경, 임종필, 홍승헌. 한방약리학. 도서출판 신일상사. 2005:268-73.
  9. 정선. 薏苡仁湯의 소염.진통 효과. 조선대학교 대학원 석사논문. 1998.
  10. 홍남두, 주수만, 정규만, 문정호. 麻杏甘石湯이 鎭痛, 解熱, 消炎, 袪痰, 摘出腸管 및 血壓과 呼吸에 미치는 영향. 생약학회지. 1986;17(3).
  11. 문영희, 노은미. 麻杏薏甘湯의 소염, 진통, 해열작용. 생약학회지. 2001;32(3).
  12. 이한창, 염미정, 김건호, 심인섭, 최강덕, 이혜정, 함대현. 흰쥐의 Adjuvant 유발 다발성 관절염에 대한 마황 약침의 치료 효과. 동의생리병리학회지. 2003;17(2):346-51.
  13. 이재희. 圖說 漢方診療要方, 醫方出版社, 2002:74-7, 140-1, 272-3, 276-82, 450-2,482-3, 553-4, 570-1.
  14. Lee TH, Kwak HB, Kim HH, Lee ZH, Chung DK, Baek NI, Kim J. Methanolextracts of Stewartia koreana inhibit cytooxygenase-2(COX-2) and induciblenitric oxide synthase(iNOS) gene expression by blocking NF-kappaB transactivation in LPS-activated RAW 264.7 cells. MOL Cells. 2007;23(3):398-404.
  15. Di Rosa M, Giroud JP, Willoughby DA. Studies of the mediators of the acuteinflammatory response induced in rats in different sites by carrageenan andturpentine, J Pathol. 1971;104:15-29. https://doi.org/10.1002/path.1711040103
  16. 박은희, 신미자. 조각자 추출물의 항염증 작용, 약학회지. 1993;37:124-8.
  17. Kiyoshi Takeda, Tsuneyasu Kaisho, and Shizuo Akira. Toll-like receptors. Annu. Rev. Immunol. 2003;21:335-76. https://doi.org/10.1146/annurev.immunol.21.120601.141126
  18. Bhattacharyya A, Pathak S, Datta S, Chattopadhyay S, Basu J, Kundu M.Mitogen-activated protein kinases and NF-kappaB regulate H. pylori-mediatedIL-8 release from macrophages. Biochem J. 2002;366:376-82.
  19. Binetruy B, Smeal T, Kariu M. Ha-Ras augments c-Jun activity and stimulatesphosphoylation of its activation domain. Nature. 1991;351:122-7. https://doi.org/10.1038/351122a0
  20. Santos, C. C. D., and Slutsky, A. S. J. App. Physiol. 2000;89:1645-55. https://doi.org/10.1152/jappl.2000.89.4.1645
  21. Su GL. Lipopolysaccharides in liver injury. molecular mechanisms of Kupffer cellactivation. American journal of physiology. 2002;283:256-65.
  22. Dos Santos CC, Slutsky AS. Invited review. mechanisms of ventilator-induced lung injury. aperspective. Journal of applied physiology. 1985;89:1645-55.
  23. Marshall JC. Inflammation, coagulopathy, and the pathogenesis of multiple organdysfunction syndrome. Critical care medicine. 2001;29:99-106. https://doi.org/10.1097/00003246-200107001-00032
  24. Shen FM, Guan YF, Xie HH, Su DF. Arterial baroreflex function determines thesurvival time in lipopolysaccharide-induced shock in rats. Shock. 2004;21:556-60. https://doi.org/10.1097/01.shk.0000126647.51109.5c
  25. 백형환 외 25인. 리핀코트의 그림으로 보는 생화학. 도서출판 신일상사. 2005:148.
  26. Wu GH, Zhang YW, Wu ZH. Modulation of postoperative immune and inflammatory response by immune-enhancing enteral diet in gastrointestinal cancer patients. World J Gastroenterol. 2001;7:357-62. https://doi.org/10.3748/wjg.v7.i3.357
  27. Galla, H. J. Nitric oxide, NO, an intracellular messenger. Angew. Chem. Int. Ed. Engl. 1993;32:378-80. https://doi.org/10.1002/anie.199303781
  28. Knowles, R. G. and Moncada, S. Nitric oxide as a signal in blood vessels. TIBS 1992;17:399-402.
  29. Nathan, C. Nitric oxide as a secretory product of mammalian cells. FASEB J.1992;6:3051-64. https://doi.org/10.1096/fasebj.6.12.1381691
  30. Stuehr, D., Cho, H. J., Kwon, N. S., Weise, M. and Nathan, C. F. Purification and characterization of the cyrokineinduced macrophage nitric oxide synthase: an FAD-and FMN-containing flavoprotein. Proc. Natl. Sci. USA. 1991;88:7773-7.
  31. McCartney-Francis, N., Allen, J. B., Mizel, D. E., Albina, J. E., Xie, Q. W., Nathan, C. F. and Wahl, S. M. Suppression of arthritis by an inhibitor of nitric oxide synthase. J. Exp. Med. 1993;178:749-54. https://doi.org/10.1084/jem.178.2.749
  32. 福州市人民醫院. 脈經校釋. 北京. 人民衛生出版社. 1984:344-485.
  33. 맹웅재. 傷寒論槪說. 원광대학교출판국. 1996:18, 65-7, 76-7, 302.
  34. 錢超塵, 校注. 唐本傷寒論. 北京. 中國中醫學出版社. 1994:1-332.
  35. 이재희. 이재희 선생의 本草講座. 의방출판사. 2004:91, 123, 187, 200-205, 375. 570.
  36. 張機 原著. 李克光 主編. 金匱要略. 北京, 人民衛生出版社. 1989:149, 411-2.
  37. 張機 原著. 李培生 主編. 傷寒論. 北京, 人民衛生出版社. 1987:38, 55, 58, 62, 70, 72, 78, 109, 472.
  38. 朴南錯. 漢方治療大全. 南山堂. 1980:335.
  39. 朴盛洙, 廉泰換. 現代漢方講座. 醫藥社. 1963:46-7.
  40. 廉秦煥 譯. 仲景方類聚. 翰林社. 1974:104.
  41. 廉泰煥. 漢方處方解說 現代漢方講座 第2卷, 1995:50-1.
  42. Kiani A, Viola JP, Lichtman AH, Rao A. Down-regulation of IL-4 gene transcription and control of Th2 cell differentiation by a mechanism involving NFAT1. Immunity 1997 Dec;7(6):849-60. https://doi.org/10.1016/S1074-7613(00)80403-3
  43. Schuh K, Kneitz B, Heyer J, Bommhardt U, Jankevics E, Berberich-Siebelt F, Pfeffer K, Muller-Hermelink HK, Schimpl A, Serfling E. Retarded thymic involution and massive germinal center formation in NFATp-deficient mice. Eur J Immunol1998 Aug;28(8):2456-66. https://doi.org/10.1002/(SICI)1521-4141(199808)28:08<2456::AID-IMMU2456>3.0.CO;2-9
  44. 朴盛洙, 廉奏煥. 現代漢方講座. 행림서원. 1996:267.
  45. 하연석. 한방처방의 구성과 해설. 약업신문사. 1996:313.
  46. 김창민, 노재섭, 문영희, 신승원, 육창수, 이경순. 임상상용방제해설. 정담. 1997:80.
  47. 진갑덕 譯. 皇漢醫學. 癸丑文化社. 1974:332.
  48. 蔡仁植. 傷寒論譯詮. 高文社. 1984:41-2.