한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제22권2호
  • /
  • Pages.19-38
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  • 2009
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
권호 표지

LPS로 활성화된 복강 대식세포에서 마황(麻黃)이 배오(配伍)된 10종(種) 처방의 NO 억제 효과

Inhibitory Effect of Ten Kinds of Herbal Medicine Including Ephedrae Herba on Nitric Oxide in LPS-induced Murine Peritoneal Macrophage

  • 배주연 (원광대학교 한의과대학 안이비인후피부과) ;
  • 조은희 (원광대학교 한의과대학 침구과) ;
  • 김훈영 (원광대학교 운영 군산의료원 산부인과) ;
  • 박민철 (원광대학교 한의과대학 안이비인후피부과)
  • 발행 : 2009.08.25
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초록

Objective : The purpose of this study is to find out whether Herba Ephedrae, solely used, and Herbal medicine in which this was included, have inhibitory effects of Nitric Oxide(NO). Methods : We tested the inhibitory effects of Nitric Oxide(NO) with Herba Ephedrae and ten kinds of Herbal medicine combined with Herba Ephedrae(Worlbikachul-Tang, 越婢加朮湯; Mahaengkamsuk-Tang, 麻杏甘石湯; Shinbi-Tang, 神秘湯; Mahwangbujaseshin-Tang, 麻黃附子細辛湯; Euiin-Tang, 薏苡仁湯; Galgeun-Tang, 葛根湯; Mahaengeuigam-Tang, 麻杏薏甘湯; Mahwang-Tang, 麻黃湯; Socheongryong-Tang, 小靑龍湯; Gaemagakban-Tang, 桂麻各半湯) on RAW264.7 cells. Results and Conclusions : 1. We carried out MTT assay on Herba Ephedrae and those decoctions including this in order to determine whether they accommodate cotytoxicity. The results were that Worlbikachul-Tang, Mahaengkamsuk-Tang, Mahwangbujaseshin-Tang, Mahaengeuigam-Tang, Mahwang-Tang, Socheongryong-Tang and Gaemagakban-Tang showed no cytotoxicity on RAW264.7 with 0.1mg/ml and 0.5mg/ml dosages of decoctions but displayed cytotoxicity on the cell with 1mg/ml. Solely used Herba Ephedrae, Shinbi-Tang, Euiin-Tang and Galgeun-Tang exhibited cytotoxicity beyond the concentration of 0.5mg/ml. 2. Worlbikachul-Tang, Mahaengkamsuk-Tang, Shinbi-Tang, Mahwangbujaseshin-Tang, Euiin-Tang, Galgeun-Tang, Mahaengeuigam-Tang, Mahwang-Tang and Socheongryong-Tang showed inhibition of NO production but solely used Herba Ephedrae and Gaemagakban-Tang did not exhibit such reaction.

키워드

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