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Physico-chemical Characteristics of Dua-Chungkukjang

두아 청국장의 이화학적 특성

  • Park, Seok-Kyu (Dept. of Food and Nutrition, Sunchon National University) ;
  • Ryu, Cha-Yeol (Dept. of Microbiological Engineering, Jinju National University) ;
  • Lee, Sang-Won (Dept. of Microbiological Engineering, Jinju National University)
  • 박석규 (순천대학교 식품영양학과) ;
  • 유차열 (진주산업대학교 미생물공학과) ;
  • 이상원 (진주산업대학교 미생물공학과)
  • Published : 2009.09.30

Abstract

Unlike the traditional preparation method for Chungkukjang, we prepared Dua-Chungkukjang with soybean fermented at $40^{\circ}C$ after the soaking, germination and steaming process, and we studied the physico-chemical characteristics of Dua-Chungkukjang. Reducing sugar content in both traditional Chungkukjang and Dua-Chungkukjang increased sharply after 10 hr of fermentation, but it decreased slightly after that. Total nitrogen content in both traditional Chungkukjang and Dua-Chungkukjang increased slowly as fermentation took place. Total nitrogen content reached 3,500 mg% and 3,700 mg%, and soluble nitrogen compound reached 750 mg% and 1,800 mg% after 40 hr of fermentation, respectively. $\beta$-Glucosidase activity increased continuously as fermentation took place, and it reached 193, 223, 235 and 248 units in Dua-Chungkukjang while it reached 125, 178, 205 and 214 units in traditional Chungkukjang after 10 hr, 20 hr, 30 hr and 40 hr of fermentation. This means that $\beta$-Glucosidase activity in Dua-Chungkukjang was $1.2{\sim}1.5$ times higher than that in the control treatment, traditional Chungkukjang. Total isoflavone content was $101.44{\mu}g$/g and $365.92{\mu}g$/g for 10 hr of fermentation, and $225.98{\mu}g$/g and $441.37{\mu}g$/g for 40 hr in traditional Chungkukjang and Dua-Chungkukjang, respectively. Isoflavone content of Dua-Chungkukjang was 2-3.5 times higher than that of traditional Chungkukjang. Anthocyanin content also increased continuously as fermentation took place, and it reached 5.0 mg/g and 6.9 mg/g in traditional Chungkukjang and Dua-Chungkukjang after 40 hr of fermentation, respectively.

종래의 전통청국장 제조방법과는 달리 원료 콩을 수침하여 균일하게 발아시켜 증자한 다음, $40^{\circ}C$에서 발효를 행한 두아청국장의 이화학적 성분 등을 검토하였다. 환원당 함량은 전통청국장 및 두아청국장 모두 발효 10 시간째까지는 급격하게 증가하였으나 그 이후부터 약간 감소하였다. 총 질소함량은 전통청국장 및 두아청국장 모두 서서히 증가하여 발효 40 시간째에 각각 약 3,500 mg%과 3,700 mg%를 나타내었으며, 수용성 질소함량은 750 mg%와 1,800 mg%를 나타내었다. 그리고 아미노태질소 함량은 발효 10시간 이후로는 아주 완만하게 증가하여 발효 40시간째에 345 mg% 및 342 mg%를 나타내었다.$\beta$-glucosidase 활성은 전통청국장에서 발효 10, 20, 30 및 40 시간째에 각각 125, 178, 205 및 214 units를 나타내었으나 두아청국장은 193, 223, 235 및 248 units를 나타내어 대조구에 비하여 $1.2{\sim}1.5$배 정도 높은 것으로 나타났다. 전통청국장 및 두아청국장의 총 isoflavone함량은 발효 10시간째에 각각 $101.44{\mu}g$/g 및 $365.92{\mu}g$/g, 40 시간째에는 $225.98{\mu}g$/g 및 $441.37{\mu}g$/g이 검출되어 두아청국장의 isoflavone함량이 전통청국장 보다 3.5배$\sim$약 2배 높은 것으로 나타났다. Anthocyanin 함량은 발효 40 시간째에 대조구인 전통청국장에서는 5.0 mg/g, 두아청국장에서는 6.9 mg/g이 검출되었다.

Keywords

References

  1. AIm, Y. S., Y. S. Kim, and D. H Shin. 2006. Isolation, identification and fermentation characteristics of Bacillus sp. with high protease activity from traditional cheongukjang. Korean J. Food SCI. Technol. 38, 82-87
  2. Anson, M. 1. 1939. The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J. Gen. Physiol. 22, 79-85 https://doi.org/10.1085/jgp.22.1.79
  3. A.O.A.C. 1990. Official methods of analysis. pp. 32-33, 15th eds., Association of official analytical chemists, Washington D.C. U.S.A
  4. Chung, K. W., Y. H. Joo, and D. J. Lee. 2004. Content and color difference of anthocyanin by different storage periods on seed coats of black soybean 'Glycine max (L.) Merr.'. Korean J. IntI. Agri. 16, 196-199
  5. Chung, K. W., Y. H. Joo, and D. J. Lee. 2004. Content and color difference of anthocyanin by different planting dates and growth stages in seed coats of black soybean [Glycine max (1.) Merr.]. Korean J. IntI. Agri. 16, 200-204
  6. Hong, S. P., J. S. Kime, C. M. Jang, S. M. Ryu, J. S. Choi, and H. J. Park. 1998. Physicochemical properties of traditional chunggukjang produced in different regions. J. Korean Soc. Appl. Biol. Chem. 41, 423-430
  7. Institute of Miso Technologists. 1968. Official methods of Miso analysis, pp. 1-34, Institute of Miso Technologists, Tokyo, Japan
  8. Joo, H K. 1996. Studies on chemical composition of commercial Chungkukjang and flavor compounds of Chungkukjang by mugwort(Artmisia asiatica) or red prpper seed oil. Korea Soybean Society 13, 44-56
  9. Joo, Y. H, K. W. Chung, and D. J. Lee. 2004. Content and color difference of anthocyanin by different seed size and cotyledon colors in black soybean 'Glycine max (1.) Merr.'. Korean J. IntI. Agri. 16, 249-252
  10. Kim, B. N., C. H. Park, B. M. Yun, M. C. Jung, and S. Y. Lee. 1995. Changes of Saccharides and amino acid in Natto added with spice during fermentation. J. Korean Soc. Food Nutr. 24, 114-120
  11. Kim, C. H, J. S. Park, H S. SohnI and C. W. Chung. 2002. Determination of isoflavone, total saponin, dietary fiber, soy oligosaccharides and lecithins from commercial soy products based on the one serving size. -Some bioactive compounds from commercialized soy products-. Korean J. Food Sci. Technol. 34, 96-102
  12. Kim, E. M., K J. Lee, and K M. Chee. 2004. Comparison on isoflavone contents between soybean and soybean sprouts of various soybean cultivas. Kor. Nutrition Society 37, 45-51
  13. Kim, S. S., K T. Kim, and H D. Hong. 2001. Development of Chunggukjang adding the sword beans. Korea soybean society 18, 33-50
  14. Kim, S. S., K T. Kim, and H D. Hong. 2001. Development of Chunggukjang adding the sword beans. Korea Food Research Institute 18, 208-214
  15. Ko, H S., D. H. Sho, S. Y. Hwang, and Y. M. kim. 1999. The effect of quality improvement by chungkuk-jang's processing methods. Korean J. Food & Nutr. 12, 1-6
  16. Lee, C. Y. 1989. Korean soy seasongings and culture. Food Science and Industry 22, 3-7
  17. Miller, G. 1. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426-432 https://doi.org/10.1021/ac60147a030
  18. Park, S. K, C. Y. Ryu, and S. W. Lee. 2008. Establishment of Preparation Method of Dua-Chungkukjang. Journal of Life Science 18, 1758-1763 https://doi.org/10.5352/JLS.2008.18.12.1758
  19. Peralta, R. M., M. K Kadowaki, H. F. Terenzi, and J. A. Jorge. 1997. A highly thermostable $\beta$-glucosidase activity from the thermophilic fungus humicola-grisca var. thermo-pedia: purification and biochemical characterization. FEMS Microbiol. Letter 146, 291-295 https://doi.org/10.1016/S0378-1097(96)00490-9
  20. Ra, K S., S. H Oh, J. M. Kim, and H J. Suh. 2004. Isolation of fibrinolitic enzyme and $\beta$-glucosidase producing strains from Doenjang and optimum conditions of enzyme production. J. Korean Soc. Food Sci. Nuri. 33, 439-442 https://doi.org/10.3746/jkfn.2004.33.2.439
  21. Shon, M. Y. 1999. Physicochemical properties and biological activities of Chungkuk-jang produced from Korean black bean. Ph.D. Dissertation, GyeongSang National University. Jinju, Korea

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