YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Assessment of Bacterial Contaminations of the Surrounding Environment which Influences to Health

건강에 영향을 주는 주변환경의 미생물 오염 실태

  • Published : 2009.08.31
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Abstract

Community-acquired antimicrobial resistant bacteria are an emerging problem whole world-wide. Generally, Hands are main mediator of pathogen transmission as compared with other body parts. So, the purpose of this research was to investigate the microbiological quality and antimicrobial susceptibilities of samples which were collected from the door knob and surface in public- and home-facilities, and also hand. Of total 191 samples, 71 samples (37%) were shown to be of high-level total bacterial count (>$10^8\;CFU/cm^2$). And presence of Staphylococcus and Enteric bacilli was observed in 61 samples (32%) and 76 samples (40%), respectively. Antimicrobial susceptibilities of staphylococcal isolates from the samples were tested for six different antimicrobial agents, which are in wide spread clinical use in Korea, as well as four new antimicrobials, daptomycin, linezolid, quinupristin/dalfopristin and tigecycline. Among staphylococcal isolates, antimicrobial resistance were observed in oxacillin (n=6), mupirocin (n=7), vancomycin (n=1), quinupristin/dalfopristin (n=2) and gentamicin (n=5). Fortunately, all the isolates were susceptible to new antimicrobial such as daptomycin, linezolid and tigecycline. Furthermore 4 Enterococcus faecalis were isolated from four hand samples, and all these isolates exhibited multidrug resistance to four different antimicrobial (oxacillin, quinupristin/dalfopristin, mupirocin, gentamicin). Also, 5 Escherichia coli were isolated from surface in home (n=3), door knob in public facilities (n=1) and hand (n=1). Escherichia coli isolated from hand was high-level resistant to tigecycline ($128{\mu}g$/ml) and gentamicin ($64{\mu}g$/ml).

Keywords

References

  1. Seo, S. H. and Ryu, K. M. : Preception of foodborne illness prevention and personal Hygiene practice. Kor. J. Food Cookery Sci. 24, 294 (2008)
  2. Kim, M. J., Lee, D. K., Jang, S., Yang, H. J., An, H. M., Baek, E. H. and Ha, N. J. : Assessment of bacterial contamination and antibiotic resistance patterns in ready-to-eat side dishes in Seoularea, 2007. Yakhak Hoeji 52, 434 (2008)
  3. Lee, H. M., Lee, G. Y., Yoon, E. K., Kim, H. J., Kang, Y. S., Lee, D. H., Park, J. S., Lee, S. H., Woo, G. J., Kang, S. H., Yang, J. S. and Yang, K. H. : Computation of maximum edible time using monitoring data of Staphylococcus aureus in kimbap and food micromodel. J. Fd. Hyg. Safety 19, 49 (2004)
  4. Kim, J. Y., Kim, J. S., Shim, W. B., Park, S. J. and Chung, D. H. : Antibiotics resistance for isolated pathogenic bacteria from manufacturing establishment of sandwich products. J. Fd. Hyg. Safety 22, 45 (2007)
  5. Weinstein, J. : The clean restaurant employee hygiene. Restaurants Inst. 101, 138 (1991)
  6. Restaine, L. and Charles, E. W. : Antimicrobial effectiveness of hand washing for food establishment, Dairy Food Environ. Sanit., 10, 136 (1990)
  7. Maryellen Guinan, Maryanne McGuckin and Sevareid A : Who washes hands after using the bathroom? AJIC Am. J. Infect. Control. 25, 424 (1997) https://doi.org/10.1016/S0196-6553(97)90092-4
  8. Manfred L. Rotter : Hand washing and hand disinfection. Hospital Epidemiology and Infection Control, 2e, 87, 1339 (1999)
  9. Doyle, M. P., Ruoff, K. L., Pierson, M., Weinberg, W., Soule, B. and Michaels, B. S. : Reducing transmission of infectious agents at home. Dairy Food Environ. Sanit. 20, 330 (2000)
  10. Bae, H. J. : Analysis of contamination of bacteria from raw materials, utensils and worker’s hands to prepared foods in food-service operations. J. Kor. Soc. Food Sci. Nutr. 35, 655 (2006) https://doi.org/10.3746/jkfn.2006.35.5.655
  11. Kang, Y. J. : Handwashing, essential for safe food preparation, a technical review. J. Kor. Public Health Assoc. 27, 269 (2001)
  12. Kim, E. M. and Kim, H. S. : Evalution of microbiological hazards of baking utensils and environment of bakeries. Kor. J. Soc. Food Cookery Sci. 7, 85 (2001)
  13. Kwak, T. K., Jang, H. J., Rew, K. and Kim S. H. : Effectiveness of 70% alcohol solution and hand washing methods on removing transient skin bacteria in foodservice operation. J. Kor. Dieter Assoc. 4, 235 (1998)
  14. Park, H. K., Kim, K. L., Shin, H. W. and Kye, S. H. : Evalution of microbiological hazards of cooking utensils and environment of mass catering establishment. J. Fd. Hyg. Safety 15, 315 (2000)
  15. Park, H. J. and Bae, H. J. : Evalution of microbiological hazards of hygiene by the customers' hands in university foodservice operation. J. Kor. Soc. Food Sci. Nutr. 35, 940 (2006) https://doi.org/10.3746/jkfn.2006.35.7.940
  16. Jack, G., Marianne, P. and Ross, D. : Evaluation of risks related to microbiological contamination of read-to-eat food by food preparation workers and the effectiveness of interventions to minimize those risks. http://www.cfsan.fda.gov (1999)
  17. Synder, O. P. : HACCP-An industry food safely self-control program-part. Dairy Food Environ. Sanit. 12, 310 (1992)
  18. Kang, Y. J. : Handwashing, essential for safe food preparation, a technical review. J. Kor. Public Health Assoc. 27, 269 (2001)
  19. Larson, E. : Skin hygiene and infection prevention, more of the same or different approaches? Clinic Infect. Dis. 29, 1287 (1999) https://doi.org/10.1086/313468
  20. Kim, E. M. and Kim, H. S. : Evaluation of microbiological hazards of baking utensils and environment of bakeries. Kor. J. Soc. Food Cookery Sci. 7, 85 (2001)
  21. Park, H. K., Kim, K. L., Shin, H. W., Kye, S. H. and Yoo, W. C. : Evaluation of microbiological hazards of cooking utensils and environment of mass catering establishments. J. Fd. Hyg. Safety 15, 315 (2000)
  22. Kwak, T. K., Jang, H. J., Rew, K. and Kim, S. H. : Effectiveness of 70% alcohol solution and hand washing methods on removing transient skin bacteria in foodservice operation. J. Kor. Dietet. Assoc. 4, 235 (1998)
  23. Lee, D. K., Hwang, J. W., Yang, H. J., Jang, S., Baek, E. H., Kim, M. J., Kim, J. H., Lee, S. J. and Ha, N. J. : Assessment of bacterial contamination of raw meats sold in Korea. J. Environ. Toxicol. 22, 313 (2007)
  24. Fuchs, P. C., Barry, A. L. and Brown, S. D. : Daptomycin susceptibility tests, interpretive criteria, quality control, and effect of calcium on invitro tests. Diagn. Microbiol. Infect. Dis. 38, 51 (2000) https://doi.org/10.1016/S0732-8893(00)00164-4
  25. Park, H. J. and Bae, H. J. : Evaluation of microbiological hazards of hygiene by the customers' hands in university foodservice operation. J. Kor. Soc. Food Sci. Nutr. 35, 940 (2006) https://doi.org/10.3746/jkfn.2006.35.7.940
  26. Chung, J. K., Kim, M. J., Kee, H. Y., Choi, M. H., Seo, J. J., Kim, S. H., Park, J. T., Kim, M. G. and Kim, E. S. : Prevalence of food poisoning bacteria on hands in various age group. J. Fd. Hyg. Safety. 23, 40 (2008)
  27. 질병관리 본부, 손 씻기 실태 및 대국민 인식도 조사 결과 보고서 (2005. 10.)
  28. Kim, H. J., Rha, Y. A. and Na, Y. S. : Research of hand sanitation level and rcognition for hand sanitizer ysage in working places (industries). Kor. J. of Culinary Research 12, 269 (2006)
  29. Bae, H. J., Paik, J. E., Joo, N. M. and Yoon, J. Y. : HACCP principle and application for food service managers. Kyomunsa, Seoul (2006)
  30. http://www.handwashing.or.kr/.
  31. Jeong, H. J., Kim, W. J., Kim, M. J. and Park, S. C. : Nosocomial infection surveillance in the intensive care unit. Kor. J. Infect. Dis. 27, 105 (1995) https://doi.org/10.3109/00365549509018988
  32. OIE, European Scientific Conference. The use of antibiotics in animals ensuring the protection of public health (1999)
  33. Kang, T. M., Cho, S. K. and Park, J. H. : Antibiotic resistances of enterococcus isolated from salad and sprout. Kor. J. Microbiol. Biotechnol. 36, 142 (2008)