YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Antioxidant Effect of Lutein on N-Nitrosodiethylamine-induced Oxidative Stress in Mice

생쥐에서 N-Nitrosodiethylamine에 의한 산화성 스트레스에 대한 Lutein의 항산화효과

  • Published : 2009.08.31
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Abstract

To investigate the antioxidant effect of lutein on N-nitrosodimethylamine (NDEA)-induced oxidative stress in mice, we measured lipid peroxidation, superoxide dismutase (SOD) and catalase of various tissues. Body weight was almost similar in lutein and control groups during 3 weeks. NDEA increased significantly the activities of typical marker enzymes of liver function (AST, ALT and ALP) in both groups. However, the increase of plasma aminotransferase activity significantly decreased in lutein group. Lipid peroxidation and SOD in various tissues, such as heart, lung, liver, kidney, spleen and plasma were significantly increased by NDEA, which were significantly reduced by lutein at a dose of 50 mg/kg. Catalase activity decreased significantly in control and lutein groups treated with NDEA, the effect being less in lutein group. Lesser effect on SOD and catalase in NDEA-treated lutein group indicates the improvement of protective mechanisms by lutein. Thus, it can be concluded from the present study that lutein can offer a useful protection against NDEA-induced oxidative stress.

Keywords

References

  1. Yeum, K. J., Taylor, A., Tang, G. and Russell, R. M. : Measurement of carotenoids, retinoids, and tocopherols in human lenses. Invest. Ophthalmol. Vis. Sci. 36, 2756 (1995)
  2. Ascherio, A., Rimm, E. B., Hernan, M. A., Giovannucci, E., Kawachi, I., Stampfer, M. J. and Willett, W. C. : Relation of consumption of vitamin E, vitamin C and carotenoids to risk for stroke among men in the United States. Ann. Intern. Med. 130, 963 (1999) https://doi.org/10.7326/0003-4819-130-12-199906150-00003
  3. Krinsky, N. I., Landrum, J. T. and Bone, R. A. : Biologic mechanisms of the protective role of lutein and zeaxanthin in the eye. Annu. Rev. Nutr. 23, 171 (2003) https://doi.org/10.1146/annurev.nutr.23.011702.073307
  4. Dwyer, J. H., Navab, M., Dwyer, K. M., Hassan, K., Sun, P., Shircore, A., Hama-Levy, S., Hough, G., Wang, X., Drake, T., Merx, C. N. and Fogelman, A. M. : Oxygenated carotenoid lutein and progression of early atherosclerosis. Circulation. 103, 2922 (2001) https://doi.org/10.1161/01.CIR.103.24.2922
  5. Jin, X. H., Ohgami, K., Shiratori, K., Suzuki, Y., Hirano, T., Koyama, Y., Yoshida, K., Ilieva, I., Iseki, K. and Ohno, S. : Inhibitory effects of lutein on endotoxin-induced uveitis in Lewis rats. Invest. Ophthalmol. Vis. Sci. 47(6), 2562 (2006) https://doi.org/10.1167/iovs.05-1429
  6. Kim, J. H., Na, H. J., Kim, C. K., Kim, J. Y., Ha, K. S., Lee, H., Chung, H. T., Kwon, H. J., Kwon, Y. G. and Kim, Y. M. : The non-provitamin A carotenoid, lutein, inhibits NF-kappaBdependent gene expression through redox-based regulation of the phosphatidylinositol 3-kinase/PTEN/Akt and NF kappaBinducing kinase pathways: role of H(2)O(2) in NF-kappaB activation. Free Radic. Biol. Med. 45(6), 885 (2008) https://doi.org/10.1016/j.freeradbiomed.2008.06.019
  7. Taniguchi, M., Yasutake, A., Takedomi, K. and Inoue, K. : Effects of N-nitrosodimethylamine (NDMA) on the oxidative status of rat liver. Arch. Toxicol. 73, 141 (1999) https://doi.org/10.1007/s002040050598
  8. Spiteller, G. : Enzymic lipid peroxidation - a consequence of cell injury? Free Radic. Biol. Med. 21, 1003 (1996) https://doi.org/10.1016/S0891-5849(96)00268-7
  9. Ohkawa, H., Ohishi, N. and Yagi, K. : Assay for lipid peroxides in animal tissues by thiobarbituric reaction. Anal. Biochem. 95, 351 (1979) https://doi.org/10.1016/0003-2697(79)90738-3
  10. Rana, S. V. S., Allen, T. and Singh, R. : Inevitable glutathione then and now. Indian. J. Exp. Biol. 40, 706 (2002)
  11. Reitman, S. and Frenkel, S. A. : Colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvate transaminases. Am. J. Pathol. 28, 56 (1957)
  12. Ames, B. N., Shigenga, M. K. and Hagen, T. M. : Oxidants, antioxidants and the degenerative diseases of aging. Proc. Natl. Acad. Sci. 90, 7915 (1993) https://doi.org/10.1073/pnas.90.17.7915
  13. Beckman, K. B. and Ames, B. N. : The free radical theory of aging matures. Physiol. Rev. 78, 547 (1998) https://doi.org/10.1152/physrev.1998.78.2.547
  14. Noguchi, N., Watanabe, A. and Shi, H. : Diverse functions of antioxidants. Free Radic. Res. 33, 809 (2000) https://doi.org/10.1080/10715760000301331
  15. Marklund, S. and Marklund, G. : Involvement of the superoxide anion radical in the auto-oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469 (1974) https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  16. King, E. J. and Delory, G. E. : The rates of enzyme hydrolysis of phosphoric esters. Biochem. J. 33, 11 (1959)
  17. Boland, A., Delapierre, D., Mossay, D., Hans, P. and Dresse, A. : Propofol protects cultured brain cells from iron ioninduced death: comparison with trolox. Eur. J. Pharmacol. 404, 21 (2000) https://doi.org/10.1016/S0014-2999(00)00496-9
  18. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. and Klenk, D. C. : Measurement of protein using bicinchoninic acid. Anal. Biochem. 163, 279 (1987) https://doi.org/10.1016/0003-2697(87)90125-4
  19. McCune, L. M. and Johns, T. : Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the Indigenous Peoples of the North American boreal forest. J. Ethnopharmacol. 82, 197 (2002) https://doi.org/10.1016/S0378-8741(02)00180-0
  20. Moreno, F. S., Toledo, L. P., de Conti, A., Heidor, R., Jordão, A. Jr., Vannucchi, H., Cardozo, M. T. and Ong, T. P. : Lutein presents suppressing but not blocking chemopreventive activity during diethylnitrosamine-induced hepatocarcinogenesis and this involves inhibition of DNA damage. Chem. Biol. Interact. 168(3), 221 (2007) https://doi.org/10.1016/j.cbi.2007.04.011
  21. Haraguchi, H., Saito, T., Ishikawa, H., Date, H., Kataoka, S. and Tamura, Y. : The physiology and pharmacology of singlet oxygen. Med. Hypothesis 60, 567 (2003) https://doi.org/10.1016/S0306-9877(03)00026-4
  22. Kohen, R. and Nyska, A. : Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions and methods for their quantification. Toxicol. Pathol. 30, 620 (2002) https://doi.org/10.1080/01926230290166724
  23. Bartsch, H., Hietanen, E. and Malaveille, C. : Carcinogenic nitrosamines: free radical aspects of their action. Free Radic. Biol. Med. 7, 637 (1989) https://doi.org/10.1016/0891-5849(89)90144-5
  24. Bansal, A. K., Bhatnagar, D. and Soni, G. L. : In vitro effect of N-nitrosodimethylamine on lipid peroxidation and antioxidant system in human erythrocytes. Toxicol. In vitro. 10, 649 (1996) https://doi.org/10.1016/S0887-2333(96)00052-5
  25. Bansal, A. K., Bansal, M., Soni, G. and Bhatnagar, D. : Modulation of NDEA induced oxidative stress by vitamin E in rat erythrocytes. Hum. Exp. Toxicol. 24, 297 (2005) https://doi.org/10.1191/0960327105ht533oa