Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effect of the BuOH Soluble Fraction of Cinnamomum camphora on Melanin Biosynthesis

녹나무 부탄올 분획물이 멜라닌 생합성에 미치는 영향

  • Ha, Sang-Keun (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Moon, Eun-Jung (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Min-Jae (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park, Hye-Min (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Yoo, Eun-Sook (Department of Pharmacology, College of Medicine, Cheju National University) ;
  • Oh, Myung-Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Kim, Sun-Yeou (Graduate School of East-West Medical Science, Kyung Hee University)
  • 하상근 (경희대학교 동서의학대학원) ;
  • 문은정 (경희대학교 동서의학대학원) ;
  • 이민재 (경희대학교 동서의학대학원) ;
  • 박혜민 (경희대학교 동서의학대학원) ;
  • 유은숙 (제주대학교 의과대학) ;
  • 오명숙 (경희대학교 약학대학) ;
  • 김선여 (경희대학교 동서의학대학원)
  • Published : 2009.08.30
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Abstract

This study was carried out to investigate the effect of Cinnamomum camphora on melanogenesis. The MeOH extract of Cinnamomum camphora inhibited mushroom tyrosinase activity in dose-dependent manner. Moreover, it significantly suppressed the melanin production in melan-a cells at the concentration of $100{\mu}/m{\ell}$. The MeOH extract was partitioned with ethyl acetate, n-butanol and water. Among them, the BuOH soluble fraction exhibited significant inhibitory effect on mushroom tyrosinase. In addition, the BuOH soluble fraction reduced the melanin production in melan-a cells. But, the BuOH soluble fraction had less inhibition effects on melan-a cell originated tyrosinase. So, it was performed western blotting for melanogenic proteins (tyrosinase, tyrosinase-related protein (TRP-2)) using melan-a cells. The BuOH soluble fraction inhibited the protein expression of tyrosinase at the concentration of $100{\mu}/m{\ell}$. The results suggested that the BuOH soluble fraction of C. camphora might be a potent inhibitor of melanin biosynthesis in melan-a cells.

Keywords

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