Antioxidative Effects of Mushroom Flammulina velutipes Extract on Polyunsaturated Oils in Oil-in-water Emulsion

  • Jang, Mi-Soon (Food and Safety Research Center, National Fisheries Research & Development Institute) ;
  • Park, Hee-Yeon (Food and Safety Research Center, National Fisheries Research & Development Institute) ;
  • Ushio, Hideki (Department of Food Science and Technology, Tokyo University of Marine Science and Technology) ;
  • Ohshima, Toshiaki (Department of Food Science and Technology, Tokyo University of Marine Science and Technology)
  • Published : 2009.06.30

Abstract

The antioxidative activities of the water solution of crude extract from edible mushroom enokitake Flammulina velutipes were compared with those of ascorbic acid and ascorbic acid 6-palmitate in oil-in-water (O/W) emulsions of cod liver oil. Oxidation of the emulsions was carried out at 40 and $50^{\circ}C$ in the dark. The antioxidant activities were measured by in vitro assay against oxygen uptake, 2-thiobarbituric acid value, hydroperoxide formation of the oils. Also, residual docosahexaenoic acid content was measured as indices of lipid oxidation. The cod liver oil in O/W emulsions with added enokitake crude extract (ECE) was significantly more stable against lipid oxidation than the control emulsions without the extract in terms of any oxidation indices used. Moreover, ECE provided remarkable antioxidative properties to eicosapentaenoic acid ethyl ester in emulsion system. These observations demonstrate that F. velutipes can be used as a natural antioxidant, which effectively prevents oxidation of polyunsaturated oils in emulsion system.

References

  1. Kaitaranta JK. Control of lipid oxidation in fish oil with various antioxidative compounds. J. Am. Oil Chem. Soc. 69: 810-813 (1992)
  2. Hettiarachchy NS, Glenn KC, Gnanasambandam R, Johnson MG. Natural antioxidant extract from fenugreek (Trigonalla foenumgraecum) for ground beef patties. J. Food Sci. 61: 516-519 (1996) https://doi.org/10.1111/j.1365-2621.1996.tb13146.x
  3. Christensen TC, Holmer G. Lipid oxidation determination in butter and dairy spreads by HPLC. J. Food Sci. 61: 486-489 (1996) https://doi.org/10.1111/j.1365-2621.1996.tb13139.x
  4. Branen AC. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52: 59-63 (1975) https://doi.org/10.1007/BF02901825
  5. Wattenberg LW. Protective effects of 2(3)-tert-butyl-4-hydroxyanisloe on chemical carcinogenesis. Food Chem. Toxicol. 24: 1099-1102 (1986)
  6. Chaudhary AK, Nokubo M, Reddy GR, Yeola SN, Morrow JD, Blair IA, Marnett LJ. Detection of endogenous malondialdehydedeoxyguanosine adducts in human liver. Science 265: 1580-1582 (1994) https://doi.org/10.1126/science.8079172
  7. Dziezak JD. Antioxidants. Food Technol. -Chicago 40: 94-102 (1986)
  8. Ramarathnam N, Osawa T, Ochi H, Kawakishi, S. The contribution of plant food antioxidants to human health. Trends Food Sci. Tech. 6: 75-82 (1995) https://doi.org/10.1016/S0924-2244(00)88967-0
  9. Wang H, Cao G, Prior RL. Oxygen radical absorbing capacity of anthrocyanins. J. Agr. Food Chem. 45: 304-309 (1997) https://doi.org/10.1021/jf960421t
  10. Hu C, Kitts DD. Antioxidant activity of echinacea root extract. J. Agr. Food Chem. 48: 1466-1472 (2000) https://doi.org/10.1021/jf990677+
  11. Pan YM, Liang Y, Wang HS, Liang M. Antioxidant activities of several Chinese medicine herbs. Food Chem. 88: 347-350 (2004) https://doi.org/10.1016/j.foodchem.2004.02.002
  12. Anna Podsedek. Natural antioxidants and antioxidant capacity of Brassica vegatables: A review. LWT− J. Food Technol. 40: 1-11 (2007) https://doi.org/10.1016/j.lwt.2005.07.023
  13. Lim YY, Lim TT, Tee JJ. Antioxidant properties of several tropical fruits: A comparative study. Food Chem. 103: 1003-1008 (2007)
  14. Asakawa Y, Hashimoto T, Tori M, Mizuno Y, Fukazawa Y. Crytoporic acids A-G, drimane-type sesquiterpenoid ethers of isocitiric acid from the fungus Cryptoporus volvatus. Phytochemistry 31: 579-592 (1992) https://doi.org/10.1016/0031-9422(92)90042-O
  15. Nukata M, Hashimoto T, Yamamoto I, Iwasaki N, Tanaka M, Asakawa Y. Neogrifolin derivatives possessing anti-oxidative activity from the mushroom Albatrellus ovinus. Phytochemistry 59: 731-737 (2002) https://doi.org/10.1016/S0031-9422(02)00050-X
  16. Elmastas M, Isildak O, Turkekul I, Temur N. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. J. Food Compos. Anal. 20: 337-345 (2007) https://doi.org/10.1016/j.jfca.2006.07.003
  17. Yang JH, Lin HC, Mau JL. Antioxidant properties of several commercial mushrooms. Food Chem. 77: 229-235 (2002) https://doi.org/10.1016/S0308-8146(01)00342-9
  18. Jang MS, Eun JB, Ushio H, Ohshima T. Antioxidative properties of mushroom Flammulina velutipes crude extract on the oxidation of cod liver oil in emulsion. Food Sci. Biotechnol. 13: 215-218 (2004)
  19. Porter WL. Recent trends in food applications of antioxidants. pp. 295-365. In: Autoxidation in Food and Biological Systems. Simic MG, Karel M (eds). Plenum Press, New York, NY, USA (1980)
  20. Porter WL. Paradoxical behavior of antioxidants in food and biological systems. pp. 93-122. In: Antioxidant: Chemical, Physiological, Nutritional, and Toxicological Aspects. Williams GM (ed). Princeton Scientific, Princeton, NJ, USA (1993)
  21. Porter WL, Black ED, Drolet AM. Use of polyamide oxidative fluorescence test on lipid emulsions: Contrast in relative effectiveness of antioxidants in bulk versus dispersed systems. J. Agr. Food Chem. 37: 615-624 (1989) https://doi.org/10.1021/jf00087a009
  22. Frankel EN, Satue-Gracia T, Meyer AS, German JB. Oxidative stability of fish and algae oils containing long-chain polyunsaturated fatty acids in bulk and in oil-in-water emulsions. J. Agr. Food Chem. 50: 2094-2099 (2002) https://doi.org/10.1021/jf0111458
  23. Ohshima T, Hopia A, German JB, Frankel EN. Determination of hydroperoxides and structures by high-performance liquid chromatography with post-column detection with diphenyl-1-pyrenylphosphine. Lipids 31: 1091-1096 (1996)
  24. AOCS. Official and Tentative Methods of the AOCS. Method Cd 19-90. American Oil Chemists' Society, Champaign, IL, USA (1995)
  25. AOCS. Official and Tentative Methods of the AOCS. Method Ce 1b-89. American Oil Chemists' Society, Champaign, IL, USA (1991)
  26. SPSS Institute. SPSS User's Guide: Statistics. Chicago, IL, USA (1999)
  27. Duh P-D. Antioxidant activity of water extract of four Harng Jyur (Chrysanthemum morifolium Ramat) varieties in soybean oil emulsion. Food Chem. 66: 471-476 (1999) https://doi.org/10.1016/S0308-8146(99)00081-3
  28. Gray J. Measurement of lipid oxidation. J. Am. Oil Chem. Soc. 55: 539-546 (1978) https://doi.org/10.1007/BF02668066
  29. Frankel EN, Huang SW, Kanner J, German JB. Interfacial phenomena in the evaluation of antioxidants: Bulk oils vs. emulsions. J. Agr. Food Chem. 42: 1054-1059 (1994)
  30. Tian LL, White PJ. Antioxidant activity of oat extract in soybean and cottonseed oils. J. Am. Oil Chem. Soc. 71: 1079-1086 (1994)
  31. Wanasundara UN, Shahidi F. Stabilization of seal blubber and menhaden oils with green tea catechins. J. Am. Oil Chem. Soc. 73: 1183-1190 (1996) https://doi.org/10.1007/BF02523382
  32. Jose N, Janardhanan KK. Antioxidant and antitumour activity of pleurotus florida. Curr. Sci. India 79: 941-943 (2000)
  33. Ikekawa T. Bunashimeji, Hypsizigus marmoreus: Antitumor activity of extracts and polysaccharides. Food Rev. Int. 11: 207-209 (1995) https://doi.org/10.1080/87559129509541034
  34. Lam SK, Ng TB. Hypsin, a novel thermostable ribosomeinactivating protein with antifungal and antiproliferative activities from fruiting bodies of the edible mushroom Hypsizigus marmoreus. Biochem. Bioph. Res. Co. 285: 1071-1075 (2001)
  35. Fu HY, Shien DE, Ho CT. Antioxidant and free radical scavenging activities of edible mushrooms. J. Food Lipids 9: 35-46 (2002) https://doi.org/10.1111/j.1745-4522.2002.tb00206.x