Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant and Antimicrobial Activities of Shark Collagens, and Inhibitory Actions on Elastase and Tyrosinase

상어 콜라겐의 항산화능, 항균성, Elastase 및 Tyrosinase 저해활성

  • Kim, Jae-Won (Faculty of Food Science and Industrial Technology, Catholic University of Daegu) ;
  • Kim, Do-Kyun (Faculty of Food Science and Industrial Technology, Catholic University of Daegu) ;
  • Park, Jin-Soo (Department of Food Science and Nutrition, Dong-A University) ;
  • Lee, Ye-Kyung (Faculty of Food Science and Industrial Technology, Catholic University of Daegu) ;
  • Beik, Kyung-Yean (Faculty of Food Science and Industrial Technology, Catholic University of Daegu) ;
  • Kim, Soon-Dong (Faculty of Food Science and Industrial Technology, Catholic University of Daegu)
  • 김재원 (대구가톨릭대학교 외식식품산업학부 식품가공학) ;
  • 김도균 (대구가톨릭대학교 외식식품산업학부 식품가공학) ;
  • 박진수 (동아대학교 식품영양학과) ;
  • 이예경 (대구가톨릭대학교 외식식품산업학부 식품가공학) ;
  • 백경연 (대구가톨릭대학교 외식식품산업학부 식품가공학) ;
  • 김순동 (대구가톨릭대학교 외식식품산업학부 식품가공학)
  • Published : 2009.06.30
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Abstract

The antioxidant and antimicrobial effects of acid-soluble and pepsin-solubilizable shark (Isurus oxyrinchus) collagens (SC) (ASSC: acid-soluble shark skin collagen, ASMC: acid-soluble shark meat collagen, PSSC: pepsin-solubilizable shark skin collagen, PSMC: pepsin-solubilizable shark meat collagen) and standard marine collagen (STMC) as materials, and the ability of these materials to inhibit tyrosinase and elastase, were investigated. The electron-donating ability of SC ($1{\sim}5\;g/mL$) was $14.91{\sim}17.21%$, which was $3.0{\sim}3.6$-fold higher than that of STMC at the same concentration. Also, the SOD(superoxide dismutase)-like activity of SC (5.80 mg/mL) was $4.67{\sim}37.28%$, thus $3.0{\sim}3.6$-fold greater than that of STMC. The MIC values of SC against Staphylococcus aureus and Salmonella enteritidis were $5{\mu}g$/disc, which were remarkably lower than that of STMC ($200{\mu}g$/disc). There was no antimicrobial activity against Escherichia coli in STMC, but the MIC against E. coli was $200{\mu}g$/disc for acid-soluble SC and $100{\mu}g$/disc for pepsin-solubilizable SC. The inhibition of tyrosinase by SC (3-5 mg/mL) was $58.95{\sim}98.16%$, $3.34{\sim}3.74$-fold higher than that of STMC ($17.67{\sim}26.25%$). Also, elastase inhibition by SC (at 1 mg/mL) was $53.33{\sim}80.0%$, $1.1{\sim}4.0$-fold greater than that of STMC. These results indicated that shark collagens may be valuable new functional materials owing to their antioxidant and antimicrobial properties, and because the inhibitory activities against elastase and tyrosinase are better than those of standard marine collagen.

상어 collagens(SC)(ASSC: 산가용성 껍질 collagen, ASMC: 산가용성 육 collagen, PSSC: pepsin 가용성 껍질 collagen, PSMC: pepsin 가용성 육 collagen)의 항산화성, 항균성, tyrosinase 및 elstase 저해활성을 표품(시판 marine collagen)과 비교하였다. SC($1{\sim}5\;mg/mL$)의 전자공여능은 $14.91{\sim}17.21%$로 표품의 $4.82{\sim}5.48%$에 비하여 $3.0{\sim}3.6$배가 높았다. SC($5{\sim}80\;mg/mL$)의 SOD활성은 4.67${\sim}37.28%$로 STMC 보다 $1.9{\sim}5.9$배가 높았다. SC의 S. aureus와 S. enteritidis에 대한 최소저해농도(MIC)는 $5{\mu}g$/disc로 표품의 $200{\mu}g$/disc보다 현저하게 낮았다. E. coli에 대한 MIC는 ASSC 및 ASMC에서는 $200{\mu}g$/disc인 반면 PSSC 및 PSMC는 $100{\mu}g$/disc이었으며 표품에서는 항균활성이 없었다. S. aureus에 대한 항균력은 PSMC가, S. enteritidis에 대한 항균력은 ASMC가, E. coli에 대한 항균력은 PSMC가 가장 높았다. SC($3{\sim}5\;mg/mL$)의 tyrosinase 저해활성은 $58.95{\sim}98.16%$로 표품의 $17.67{\sim}26.25%$보다 $3.34{\sim}3.74$배가 높았다. SC의 elastase 저해활성은 농도가 0.5 mg/mL에서 1 mg/mL으로 높아짐에 따라 비례적으로 증가하였고 1 mg/mL에서의 활성도는 $53.33{\sim}80.00%$로 STMC의 50.67% 보다 높았으며 PSSC에서 가장 높은 저해활성을 나타내었다. 산 및 pepsin 가용성의 모든 상어 collagen은 STMC에 비하여 $1.1{\sim}4.0$배의 높은 활성을 나타내었다. 이상의 결과 상어 collagens은 시판 marine collagen보다 항산화성, 항균성, tyrosinase 및 elastase 저해활성이 우수하여 새로운 기능성 소재로서의 활용성이 기대된다.

Keywords

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