Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Seminal Tumor Necrosis Factor-alpha Level and Sperm Nuclear DNA Integrity in Healthy Donors

정액 내 Tumor Necrosis Factor-alpha 농도와 정자 DNA 손상과의 관련성

  • Kim, Hyun-Jun (Department of Obstetrics and Gynecology, School of Medicine, Konkuk University) ;
  • Jee, Byung-Chul (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Moon, Jeong-Hee (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Lee, Jung-Ryeol (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Suh, Chang-Suk (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Kim, Seok-Hyun (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University)
  • 김현준 (건국대학교 의학전문대학원 산부인과학교실) ;
  • 지병철 (분당서울대학교병원 산부인과) ;
  • 문정희 (분당서울대학교병원 산부인과) ;
  • 이정렬 (분당서울대학교병원 산부인과) ;
  • 서창석 (분당서울대학교병원 산부인과) ;
  • 김석현 (서울대학교 의과대학 산부인과학교실)
  • Published : 2009.03.31
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Abstract

Objectives: Seminal concentration of tumor necrosis factor-alpha (TNF-${\alpha}$) relevant to sperm nuclear DNA integrity has not been studied. The present study aimed to evaluate seminal concentration of TNF-${\alpha}$ in correlation with sperm parameters and nuclear DNA integrity in asymptomatic healthy donors. Methods: Semen samples were obtained by masturbation from forty-five healthy donors. Results: Sperm quality was assessed by computer-assisted semen analysis and nuclear DNA integrity measured by the TUNEL assay in raw semen. TNF-${\alpha}$ concentrations were measured by ELISA in frozen-thawed seminal plasmas. Sperm DNA fragmentation rates were ranged between 1.9% and 53.0% (mean${\pm}$SD, 12.4${\pm}$9.6%). Univariate analysis revealed that DNA fragmentation rate was not associated with sperm concentration or motility but had a correlation with linearity negatively (r=-0.325, p=0.03) and age positively (r=0.484, p=0.001). The mean seminal concentration of TNF-${\alpha}$ was 4.9 pg/mL with a range from 1.1 to 22.6 pg/mL. The TNF-${\alpha}$ concentration had no correlation with clinically relevant parameters of sperm quality or nuclear DNA fragmentation rate. Conclusion: Our results indicate that sperm nuclear DNA fragmentation may be not associated with seminal TNF-${\alpha}$ level or sperm quality in asymptomatic healthy donors.

목 적: 정액 내 tumor necrosis factor-alpha (TNF-${\alpha}$) 농도와 정자 DNA 손상 및 정액 검사 소견과의 관련성을 평가하고자 하였다. 연구방법: 정액 표본은 45명의 건강한 남성에서 자위에 의하여 획득하였다. 정자의 상태는 컴퓨터 정액 분석기를 이용하여 판정하였으며, 두부의 DNA 손상은 TUNEL 분석방법에 의해 측정하였다. TNF-${\alpha}$ 농도는 동결-융해된 정장액에서 ELISA법으로 측정하였다. 결 과: 정자 DNA 손상율은 1.9%에서 53% (mean ${\pm}$ SD, 12.4${\pm}$9.6%)로 매우 광범위하게 나타났다. 단변량분석에 의하면 DNA 손상 정도와 정자의 농도, 운동성과는 관련이 없었으나, 직진운동성 (linearity)과는 음의 상관 관계를 나타내었으며 (r=-0.325, p=0.03) 연구 대상 남성의 연령과는 양의 상관 관계를 나타내었다 (r=0.484, p=0.001). 정액내에 존재하는 TNF-${\alpha}$ (>1 pg/mL)는 연구 대상 남성의 73.3% (33/45)에서 검출되었으며 평균 농도는 4.9 pg/mL, 범위는 1.1에서 22.6 pg/mL이었다. 정액 검사 상의 정자 상태와 정자 DNA 손상과는 유의한 관련성이 나타나지 않았다. 결 론: 본 연구에서는 정자 DNA의 손상이 남성의 연령과 관련성이 있음을 확인하였으나, TNF-${\alpha}$와의 관련성은 확인할 수 없었다.

Keywords

References

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