말에서 개체식별 및 친자확인을 위한 ISAG Microsatellite Marker의 유용성 및 표준화

Standardization and Usefulness of ISAG Microsatellite Markers for Individual Identification and Parentage Verification in Horse Breeds

  • Kwon, Do-Yeon (College of Veterinary Medicine, Kyungpook National University) ;
  • Cho, Gil-Jae (Research Institute of Horse Industry, Kyungpook National University)
  • 발행 : 2009.06.30

초록

말에서 개체식별 및 친자확인을 위한 ISAG microsatellite marker의 적용을 위한 유용성 및 표준화를 위해 더러브렛종 6두를 포함한 다양한 품종의 20두를 대상으로 22개의 ISAG microsatellite marker를 분석한 결과 대립유전자의 수는 4개에서 8개로 분포하였고 더러브렛종에서 출현하는 대립유전자의 대부분이 비더러브렛종에서도 출현하였다. 본 연구결과를 통해서 ISAG microsatellte marker를 제주말의 혈통등록에 적용할 수 있음을 알 수 있었다.

The present study demonstrates a new approach that enables effective horse parentage testing using 22 ISAG microsatellite markers involving 6 heads of Thoroughbred horse(TB) and non-TB. In the comparison allele distribution between these horses, the alleles found in the TB were numerously detected in the non-TB. As results, we confirmed that these ISAG microsatellite markers might apply the pedigree registration of Korean native horse(Jeju horse).

키워드

참고문헌

  1. Binns MM, Uolmes NG, Holliman AM. The identification of polymorphic microsatellite loci in the horse and their use in thoroughbred parentage testing. Brit Vet J 1995; 151: 9-15 https://doi.org/10.1016/S0007-1935(05)80057-0
  2. Breen M, Lindgren G, Binns MM, Norman J, Irvin Z, Bell K, Sandberg K, Ellegren H. Genetical and physical assignments of equine microsatellites-first integration of anchored markers in horse genome mapping. Mamm Genome 1997; 8: 267-273 https://doi.org/10.1007/s003359900407
  3. Bowling AT, Eggleston-Scott ML, Byrns G, Clark RS, Dileanis S, Wictum E. Validation of microsatellite markers for routine horse parentage testing. Anim Genet 1997; 28: 247-252 https://doi.org/10.1111/j.1365-2052.1997.00123.x
  4. Cho GJ. Genetic relationship and characteristics using microsatellite DNA loci in horse breeds. J Life Sci 2007; 17: 699-705
  5. Cho GJ. Genetic relationship among the Korean native and alien horses estimated bu microsatellite polymorphism. Asian- Aust J Anim Sci 2006; 19: 784-788
  6. Cho GJ, Cho BW. Validation of microsatellite markers for routine canine parentage testing in Korea. Korean J Genet 2003; 25: 103-108
  7. Cho GJ, Cho BW. Microsatellite DNA Typing using 16 markers for parentage verification of the Korean native horse. Asian-Aust J Anim Sci 2004; 17: 750-754
  8. Coogle L, Bailet E, Reid R, Russ M. Equine dinucleotide repeat polymorphisms at loci LEX002, -003, -004, -005, -007, -008, -009, -010, -011, -013 and -014. Anim Genet 1996; 27: 126-127
  9. Dimsoski P. Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses. Croat Med J 2003; 44: 332-335
  10. Eggleston-Stott M, L DelValle A, Bautista M, Dileanis D, Wictum E, Bowling AT. Nine equine dinucleotide repeats at microsatellite loci UCDEQ136, UCDEQ412, UCDEQ425, UCDEQ437, UCDEQ467, UCDEQ487, UCDEQ502 and UCDEQ505. Anim Genet 1997; 28: 370-371 https://doi.org/10.1111/j.1365-2052.1997.tb03270.x
  11. Ellegren H, Johansson M, Sandberg K, Andersson L. Cloning of highly polymorphic microsatellites in the horse. Anim Genet 1992; 23: 133-142
  12. Glowatzki-Mullis ML, Gaillard C, Wigger G, Feies R. Microsatellite-based parentage control in cattle. Anim Genet 1995; 26: 7-12
  13. Guerin G, Bertaud M, Amigues Y. 1994. Characterization of seven new horse microsatellites: HMS1, HMS2, HMS3, HMS5, HMS6, HMS7 and HMS8. Anim Genet 1994; 25: 62 https://doi.org/10.1111/j.1365-2052.1994.tb00072.x
  14. Irvin Z, Giffard J, Brandon R, Breen M, Bell K. 1998, Equine dinucleotide repeat polymorphisms at loci ASB21, 23, 25 and 37-43, Anim Genet 1998; 29: 67
  15. Ji HJ, Kim EH, Lee KK, Kang TY, Lee JM, Shin HD, Kim LH, Yun YM. Beagle dogs parentage testing by using 22 ISAG microsatellite markers. Korean J Vet Res 2007; 47: 457-460
  16. Kemp SJ, Hishida O, Wambugu J, Rink A, Longeri ML, Ma RZ, Da Y, Lewin HA, Barendse W, Teale AJ. A panel of polymorphic bovine, ovine and caprine microsatellite markers. Anim Genet 1995; 26: 299-306.
  17. Lear TL, Brandon R, Bell K, Physical mapping of ten equine dinucleotide repeat microsatellites, Anim Genet 1999; 30: 235
  18. Lee SY, Cho GJ. Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing. J Vet Sci 2006; 7: 63-67
  19. Lipinski MJ, Amigues Y, Blasi M, Broad TE, Cherbonnel C, Cho GJ, Corley S, Daftari P, Delattre DR, Dileanis S, Flynn JM, Grattapaglia D, Guthrie A, Harper C, Karttunen PL, Kimura H, Lewis GM, Longeri M, Meriaux JC, Morita M, Morrin-O’Donnell RC, Niini T, Pedersen NC, Perrotta G, Polli M, Rittler S, Schubbert R, Strillacci MG, Van Haeringen H, Van Haeringen W, Lyons LA. An international parentage and identification panel for the domestic cat (Felis catus). Anim Genet 2007; 38: 371-377 https://doi.org/10.1111/j.1365-2052.2007.01632.x
  20. Litt M, Luty JA. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am J Hum Genet 1989; 44: 397-401
  21. Marklund S, Ellegren H, Eriksson S, Sandberg K, Andersson L. Parentage testing and linkage analysis in the horse using a set of highly polymorphic microsatellites. Anim Genet 1994; 25: 19-23 https://doi.org/10.1111/j.1365-2052.1994.tb00442.x
  22. Nechtelberger D, Kaltwasser C, Stur I, Meyer JN, Brem G, Mueller M, Mueller S. DNA microsatellite analysis for parentage control in Austrian pigs. Anim Biotech 2001; 12: 141-144 https://doi.org/10.1081/ABIO-100108340
  23. Putnova L, Knoll A, Dvorak V, Dvorak J. A novel porcine microsatellite panel for the identification of individuals and parentage control in the Czech Republic. Czech J Anim Sci 2003; 48: 307-314
  24. Siegal M. UC Davis book of horses : A complete medical reference guide for horses and foals 1st ed, New York: Haper Collins, 1996: 126-134
  25. Tozaki T, Kakoi H, Mashima S, Hirota K, Hasegawa T, Ishida N, Miura N, Tomita M. The isolation and characterization of 18 equine microsatellite loci, TKY272-TKY289. Anim Genet 2000; 31: 149 https://doi.org/10.1046/j.1365-2052.2000.00596.x
  26. Tozaki T, Kakoi H, Mashima S, Hirota KI, Hasegawa T, Ishida N, Miura N, Choi-Miura NH, Tomita M. Population study and validation of paternity testing for Thoroughbred horses by 15 microsatellite loci. J Vet Med Sci 2001; 63: 1191-1197 https://doi.org/10.1292/jvms.63.1191
  27. Tozaki T, Kakoi H, Mashima S, Hirota K, Hasegawa T, Ishida N, Miura N, Tomita M. 2000. The isolation and characterization of 34 equine microsatellite loci, TKY290- TKY323. Anim Genet 2000; 31: 234-236
  28. Van Haeringen H, Bowling AT, Scott ML, Lenstra JA, Zwaagstra KA. A highly polymorphic horse microsatellite locus: VHL20. Anim Genet 1994; 25: 207 https://doi.org/10.1111/j.1365-2052.1994.tb00129.x
  29. Weber JL, May PE. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet 1989; 44: 388-396