Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Proteomic Approach of the Protein Profiles during Seed Maturation in Common Buckwheat (Fagopyrum esculentum Moench.)

  • Park, Min-Hwa (Dept. of Crop Science, Chungbuk National University) ;
  • Shin, Dong-Hoon (Dept. of Crop Science, Chungbuk National University) ;
  • Han, Myoung-Hae (Dept. of Crop Science, Chungbuk National University) ;
  • Yun, Young-Ho (Department of Crop Science, National Institute of Highland Agriculture) ;
  • Bae, Jeong-Sook (Dept. of Crop Science, Chungbuk National University) ;
  • Lee, Yun-Sang (Division of Crop Science, Chungbuk Province Agricultural Research and Extension Services) ;
  • Chung, Keun-Yook (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Lee, Moon-Soon (Department of Industrial Plant Science & Technology, Chungbuk National University) ;
  • Woo, Sun-Hee (Dept. of Crop Science, Chungbuk National University)
  • Published : 2009.06.30
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Abstract

Single seeds of common buckwheat cultivar Suwon No. 1 when subjected to SDS-PAGE revealed very high polymorphism. High variation existed for protein or protein subunits with molecular weight 54-47kDa, 45-25kDa and 16-11kDa. The electrophoregram showed variation for globulin as well as other protein fractions. About 300 proteins were separated by two-dimensional electrophoresis in common buckwheat (Fagopyrum esculentum Moench.) seed. Seed maturation is a dynamic and temporally regulated phase of seed development that determines the composition of storage proteins reserves in mature seeds. Buckwheat seeds from 5, 10, 15, 20, and 25 days after pollination and matured stage were used for the analysis. This led to the establishment of high-resolution proteome reference maps, expression profiles of 48 spots. It was identified 48 proteins from MALDI-TOF/MS analysis of wild buckwheat seed storage proteins. The 48 proteins were found identical or similar to those of proteins reported in buckwheat and other plants; it is belonging to 9 major functional categories including seed storage proteins, stress/defense response, protein synthesis, photosynthesis, allergy proteins, amino acid, enzyme, metabolism, and miscellaneous. It appears that the major allergenic storage protein separated played the important role in buckwheat breeding and biochemical characterization.

Keywords

References

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