The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Molecular Analysis of Korean Isolate of Barley mild mosaic virus (Iks Isolate)

  • Choi, Min-Kyung (Division of Agri-Food and Horticulture, Jeollabuk-Do Agricultural Research and Extension Services) ;
  • Kamala-Kannan, Seralathan (Division of Biotechnology, Chonbuk National University) ;
  • Oh, Byung-Taek (Division of Biotechnology, Chonbuk National University) ;
  • Park, Jong-Chul (Honam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Gun-Woong (Division of Biotechnology, Chonbuk National University) ;
  • Lee, Kui-Jae (Division of Biotechnology, Chonbuk National University) ;
  • Park, Yool-Jin (Department of Environmental Landscape Architecture-Design, Chonbuk National University)
  • Published : 2009.06.30
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Abstract

The complete nucleotide sequences of both RNA of an isolated Barley mild mosaic virus (BaMMV) from Iksan, Korea, have been determined. RNA1 was 7273 nucleotides long and encodes for a polyprotein of 2261 amino acids, which contains the eight putative functional proteins. RNA2 was 3520 nucleotides long and encodes for a polyprotein of 894 amino acids, which contains two functional proteins. Results of multiple sequence alignment showed 92.9% similarity with Na1 isolate, followed by Sil, UK(F), Asl1, Remis M and UK(M) isolates, respectively. Comparison of the BaMMV-Iks polyproteins with the corresponding proteins of BaMMV-Na1 isolates showed 95% amino acid sequence identity. The phylogenetic analysis revealed that Iks isolate was closely related to Na1 strain and have a common origin.

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References

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