Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Expressional Comparison of Glucose Cotransporter Isoforms in the Rat Epididymis During Postnatal Development

  • Lee, Dong-Mok (School of Biotechnology, Yeungnam University) ;
  • Seo, Hee-Jung (Department of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University) ;
  • Son, Chan-Wok (Department of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University) ;
  • Lee, Yong-Ho (Department of Biomedical Science, Catholic University of Daegu) ;
  • Choi, In-Ho (School of Biotechnology, Yeungnam University) ;
  • Chun, Tae-Hoon (School of Life Sciences and Biotechnology, Korea University) ;
  • Cheon, Yong-Pil (School of Life Sciences and Chemistry, Institute of Basic Sciences, Sungshin Women's University) ;
  • Lee, Ki-Ho (Department of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University)
  • Received : 2009.10.28
  • Accepted : 2009.12.01
  • Published : 2009.12.01
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Abstract

Glucose is a major source of metabolic fuel and lipid and protein syntheses. Transport of glucose into the cell is regulated by an action of glucose transport.associated transporters, especially solute carriers 2A (Slc2a, protein symbol GLUT). The present study was focused on examination of mRNA expression of various Slc2a isoforms in the epididymis during postnatal development. Total RNAs isolated from different epididymal segments (caput, corpus, and caudal epididymis) were utilized for real-time polymerase chain reaction analyses. Results showed that Slc2a 1, 3, 4, 5, and 8 were expressed in the entire epididymal regions. In addition, the abundance of these Slc2a isoforms' transcripts was different within each epididymal regions. Moreover, the present study showed differential expression of these Slc2a isoforms among different epididymal segments according to postnatal ages. The current study suggests that glucose transport in the epididymis via various Slc2a isoforms would be necessary for maintenance of the epididymal functions.

Keywords

References

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