Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Differential Proteome Expression of In vitro Proliferating Bovine Satellite Cells from Longissimus Dorsi, Deep Pectoral and Semitendinosus Muscle Depots in Response to Hormone Deprivation and Addition

  • Rajesh, Ramanna Valmiki (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.) ;
  • Kim, Seong-Kon (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.) ;
  • Park, Mi-Rim (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.) ;
  • Park, Min-Ah (Dept. of Agricultural Science, Korea National Open University) ;
  • Jang, Eun-Joung (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.) ;
  • Hong, Seung-Gu (Nutrition and Physiology Team, National Institute of Animal Science, R.D.A.) ;
  • Chang, Jong-Soo (Dept. of Agricultural Science, Korea National Open University) ;
  • Yoon, Du-Hak (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.) ;
  • Kim, Tae-Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.) ;
  • Lee, Hyun-Jeong (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, R.D.A.)
  • Received : 2009.12.02
  • Accepted : 2009.12.21
  • Published : 2009.12.01
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Abstract

The aim of this study was to analyze the proteome of proliferating bovine satellite cells from longissimus dorsi, deep pectoral and semitendinosus muscle depots which had been subjected to hormonal deprivation or addition in culture. For hormone deprivation or addition studies, the cells were either grown in 10% charcoal-dextran stripped fetal bovine serum (CD-FBS) or in 10% FBS supplemented medium. Further to analyze the effect of insulin like growth factor (IGF-1) and testosterone (TS), the cells were grown in 10% CD-FBS containing IGF-1 (10 ng/ml) or TS (10 nM). Results have shown that hormone deprivation had a negative impact on proliferation of the cells from each of the muscle depots. In case of IGF-1 and TS addition, the proliferation levels were low compared with that of the cells grown in 10% FBS. Hence, to gain the insights of the proteins that are involved in such divergent levels of proliferation, the proteome of such satellite cells proliferating under the above mentioned conditions were analyzed using 2D-DIGE and MALDI-ToF/ToF. Thirteen proteins during hormone deprivation and nine proteins from hormone addition were found to be differentially expressed in all the cultures of the cells from the three depots. Moreover, the results highlighted in this study offer a role for each differentially expressed protein with respect to its effect on positive or negative regulation of cell proliferation.

Keywords

References

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