미생물학회지 (Korean Journal of Microbiology)

  • 제45권2호
  • /
  • Pages.126-132
  • /
  • 2009
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

서울시내 수계시설에서 분리된 Legionella spp.의 병원성에 대한 분자역학적 연관성

Molecular Epidemiological Relationship of the Pathogenicity of Legionella spp. Isolated from Water Systems in Seoul

  • 김진아 (서울특별시 보건환경연구원 역학조사팀) ;
  • 정지헌 (서울특별시 보건환경연구원 역학조사팀) ;
  • 김수진 (서울특별시 보건환경연구원 역학조사팀) ;
  • 진영희 (서울특별시 보건환경연구원 역학조사팀) ;
  • 오영희 (서울특별시 보건환경연구원 역학조사팀) ;
  • 한기영 (서울특별시 보건환경연구원 역학조사팀)
  • Kim, Jin-Ah (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jung, Ji-Hun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Soo-Jin (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jin, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Oh, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Han, Gi-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 투고 : 2009.03.27
  • 심사 : 2009.04.28
  • 발행 : 2009.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

레지오넬라균은 치명적인 폐렴을 일으킬 수 있는 균으로서, 기후 온난화와 함께 노출의 수위가 높아지고 있는 병원성균이다. 본 연구는 2008년 6월부터 8월까지 서울시내 25개구 소재 수계시설에서 분리한 레지오넬라균 73개 주에 대해 혈청형, Dot/Icm라 일컬어지는 병원성 유전자 분석 그리고 Pulsed field gel electrophoresis (PFGE)를 실시하여 유전자형과 그들 사이의 상관관계를 파악하였다. 73개주 중 69주는 Legionella pneumophila로 혈청형의 분포는 sg1 43주, sg6 9주, sg5 8주, sg3 8주, sg2 1주로 파악되었으며, Legionella spp. 4개주는 Legionella nautarum였다. 분리된 Legionella pneumophila 대부분이 여러 개의 병원성 유전자를 보유하고 있었으며, 반면에 Legionella nautarum은 병원성 유전자가 많이 결핍되어있었다. PFGE pattern을 분석해 볼 때, Legionella pneumophila가 동일한 혈청형안에서 다양하게 분화되어감을 볼 수 있었으며, 병원성 유전자의 분포와 깊은 상관관계를 보였다.

Legionella spp. is the causative agent of Legionellosis, which induces a potentially fatal form of pneumonia. With a concentrated performance during the summer of 2008, we secured 73 isolates from the water systems of 25 wards in Seoul. We analysed serotypes, pathogenic genes (Dot/Icm), and patterns of pulsed-field gel electrophoresis (PFGE) in an attempt to confirm relationships among them. Different from the previous year's pattern (2007), among the isolates, 69 were Legionella pneumophila and 4 were Legionella spp. The serotype distribution of Legionella pneumophila was sg1 43, sg6 9, sg5 8, sg3 8, and sg2 1. The serotype for the 4 Legionella spp. was Legionella nautarum. Most of the Legionella pneumophila had several pathogenic genes. On the other hand, the 4 Legionella spp. were defective in pathogenicity in genomic terms. The PFGE patterns of the serotypes showed a tendency for diversity of Legionella pneumophila and a close correlation with genetic pathogenicity.

키워드

참고문헌

  1. Amemura-Maekawa, J., F. Kura, B. Chang, and H. Watanabe. 2005. Legionella pneumophila serogroup 1 isolates from cooling towers in Japan form a distinct genetic cluster. Microbiol. Immunol. 49, 1027-1033 https://doi.org/10.1111/j.1348-0421.2005.tb03699.x
  2. Berger, K.H., J.J. Merriam, and R.R. Isberg. 1994. Altered intracellular targeting properties associated with mutations in the Legionella pneumophila dotA gene. Mol. Microbiol. 14, 809-822 https://doi.org/10.1111/j.1365-2958.1994.tb01317.x
  3. Brand, B.C., A.B. Sadosky, and H.A. Shuman. 1994. The Legionella pneumophila icm locus: a set of genes required for intracellular mutiplication in human macrophages. Mol. Microbiol. 14, 797-808 https://doi.org/10.1111/j.1365-2958.1994.tb01316.x
  4. Cianciotto, N.P. 2001. Pathogenicity of Legionella pneumophila. Int. J. Med. Microbiol. 291, 331-343 https://doi.org/10.1078/1438-4221-00139
  5. Cianciotto, N.P., B.I. Eisenstein, C.H. Mody, and N.C. Engleberg. 1990. A mutation in the mip gene results in an attenuation of Legionella pneumophila virulence. J. Infect Dis. 162, 121-126 https://doi.org/10.1093/infdis/162.1.121
  6. Denka Seiken Co. 1998. Bacteriology product information. Denka Seiken Co. Ltd.
  7. Field, B.S., R.F. Benson, and R.E. Besser. 2002. Legionella and Legionnaires' disease: 25 Years of investigation. Clin. Microbiol. Rev. 15, 506-526 https://doi.org/10.1128/CMR.15.3.506-526.2002
  8. Fliermans, C.B., W.B. Cherry, L.H. Orrison, S.J. Smith, D.L. Tison, and D.H. Pope. 1981. Ecological distribution of Legionella pneumophila. Appl. Environ. Microbiol. 41, 9-16
  9. Gal-Mor, O., T. Zusman, and G. Segal. 2002. Analysis of DNA regulatory elements required for expression of the Legionella pneumophila icm and dot virulence genes. J. Bacteriol. 184, 3823-3833 https://doi.org/10.1128/JB.184.14.3823-3833.2002
  10. Khler, R., J. Franghnel, B. Knig, E. Lneberg, and M. Frosch. 2003. Biochemical and functional analyses of the Mip protein: Influence of the N-terminal half and of peptidylprolyl isomerase activity on the virulence of Legionella pneumophila. Infect. Immun. 71, 4389-4397 https://doi.org/10.1128/IAI.71.8.4389-4397.2003
  11. Lee, H.K., H.S. Yang, S.R. Hong, M.S. Park, S.K. Sim, B.C. Lee, and M.Y. Park. 2001. Molecular typing of Legionella pneumophila Korean isolates from 1985 to 2001. The Report of National Institute of Health 38, 18-30
  12. Marra, A., S.J. Blander, M.A. Horwitz, and H.A. Shuman. 1992. Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages. Proc. Natl. Acad. Sci. USA 89, 9607-9611 https://doi.org/10.1073/pnas.89.20.9607
  13. Molmeret, M., M. Santic, R. Asare, R.A. Carabeo, and Y. Abu-Kwaik. 2007. Rapid escape of the dot/icm Mutants of Legionella pneumophila into the cytosol of mammalian and protozoan cells. Infect. Immun. 75, 3290-3304 https://doi.org/10.1128/IAI.00292-07
  14. Nintasen, R., F. Utrarachkij, K. Siripanichgon, A. Bhumiratana, Y. Suzuki, and O. Suthienkul. 2007. Enhancement of Legionella pneumophila culture isolation from microenvironments by macrophage infectivity potentiator (mip) gene-specific nested polymerase chain reaction. Microbiol. Immunol. 51, 777-785 https://doi.org/10.1111/j.1348-0421.2007.tb03967.x
  15. Segal, G., M. Purcell, and H.A. Shuman. 1998. Host cell killing and bacterial conjugation required overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome. Proc. Natl. Acad. Sci. USA 95, 1669-1674 https://doi.org/10.1073/pnas.95.4.1669
  16. Seung, H.J., J.H. Jung, S.J. Kim, Y.H. Jin, S.M. Lee, M.S. Kim, and J.G. Kim. 2007. Molecular epidemiological study of Legionella pneumophila isolated from water systems in Seoul. The Report of Seoul Metropolitan Government Research Institute of Public Health and Environment 43, 283-293
  17. Terry Alli, O.A., S. Zink, N.K. von Lackum, and Y. Abu-Kwaik. 2003. Comparative assessment of virulence traits in Legionella spp. Microbiology 149, 631-641 https://doi.org/10.1099/mic.0.25980-0
  18. Vogel, J.P., H.L. Andrews, S.K. Wong, and R.R. Isberg. 1998. Conjugative transfer by the virulence system of Legionella pneumophila. Science 279, 873-876 https://doi.org/10.1126/science.279.5352.873
  19. Vogel, J.P. and R.R. Isberg. 1999. Cell biology of Legionella pneumophila. Curr. Opin. Microbiol. 2, 30-34 https://doi.org/10.1016/S1369-5274(99)80005-8
  20. Zink, S.D., L. Pedersen, N.P. Cianciotto, and Y. Abu-Kwaik. 2002. The Dot/Icm type IV secretion system of Legionella pneumophila is essential for the induction of apoptosis in human macrophages. Infect. Immun. 70, 1657-1663 https://doi.org/10.1128/IAI.70.3.1657-1663.2002