미생물학회지 (Korean Journal of Microbiology)

  • 제45권3호
  • /
  • Pages.251-256
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  • 2009
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

RT-PCR법을 이용한 백합 바이러스 LSV, LMoV, CMV의 검출

Detection of Lily symptomless virus, Lily mottle virus, and Cucumber mosaic virus from Lilium Grown in Korea by RT-PCR

  • 임지현 (단국대학교 첨단과학대학 미생물학과) ;
  • 배은혜 (단국대학교 첨단과학대학 미생물학과) ;
  • 이용진 (단국대학교 첨단과학대학 미생물학과) ;
  • 박성한 (단국대학교 첨단과학대학 미생물학과) ;
  • 이규준 (단국대학교 첨단과학대학 미생물학과) ;
  • 김새로미 (단국대학교 첨단과학대학 미생물학과) ;
  • 정용태 (단국대학교 첨단과학대학 미생물학과)
  • Lim, Ji-Hyun (Department of Microbiology, and Institute of Basic Science, College of Advanced Science, Dankook University) ;
  • Bae, Eun-Hye (Department of Microbiology, and Institute of Basic Science, College of Advanced Science, Dankook University) ;
  • Lee, Yong-Jin (Department of Microbiology, and Institute of Basic Science, College of Advanced Science, Dankook University) ;
  • Park, Sung-Han (Department of Microbiology, and Institute of Basic Science, College of Advanced Science, Dankook University) ;
  • Lee, Kyu-Jun (Department of Microbiology, and Institute of Basic Science, College of Advanced Science, Dankook University) ;
  • Kim, Sae-Ro-Mi (Department of Microbiology, and Institute of Basic Science, College of Advanced Science, Dankook University) ;
  • Jung, Yong-Tae (Department of Microbiology, and Institute of Basic Science, College of Advanced Science, Dankook University)
  • 투고 : 2009.07.06
  • 심사 : 2009.08.03
  • 발행 : 2009.09.30
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초록

2008~2009년에 강원, 충남, 제주 지역의 백합 재배 농가에서 바이러스 감염 증상을 보이는 백합의 잎과 구근을 채취하였으며 RT-PCR 방법으로 Lily mottle virus (LMoV), Lily symptomless virus (LSV), Cucumber mosaic virus (CMV) 등 3 종류의 바이러스를 검출 하였다. LSV 12주, LMoV 20주, CMV 1주가 검출 되었으며 LSV에 감염된 12개의 식물체 중 7개에서는 LMoV도 검출되어 복합 감염된 것을 확인하였다. LMoV와 LSV에 의한 복합 감염은 엽맥투명화, 잎말림, 반점, 모자이크, 황화 줄무늬 등 단독 감염보다 심각한 병징을 나타내었으며 좋지 않은 환경에서 저장된 구근에서도 복합 감염이 관찰되었다. 채집된 식물체는 LMoV 감염이 가장 많았으며 Lily virus X(LVX)에 의한 감염은 검출되지 않았다. 7개 분리주의(LMoV 4주, LSV 2주, CMV 1주) 외피 단백질 유전자를 증폭한 후 염기서열을 분석하여 국내에서 발표된 백합 바이러스 염기서열(LSV:AJ516059, CMV: AJ296154)과 비교 하였으며 LMoV는 국내에서 처음으로 염기서열을 결정하여 기존에 보고된 염기서열(AJ564636)과 비교하였다. 분리주는 기존에 보고된 바이러스와 95~99%의 뉴클레오티드 염기서열 유사성을 보였으며, 이들 분리주의 분자 생물학적 특성을 밝히고 신속하고 정확한 바이러스 진단을 위해서는 전체 염기 서열 분석이 필요한 것으로 판단되었다.

Leaf samples and bulbs showing characteristic symptoms of virus infection were collected from Gang-won, Chung-nam, and Jeju Province of Korea in 2008-2009. Three viruses, Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) were detected by RT-PCR. Virus-infected plant samples were identified; 12 plants with LSV, 20 plants with LMoV, and 1 plant with CMV. Of the twelve LSV infected samples, seven samples were found to be mix-infected with LMoV and LSV. Symptoms of LMoV and LSV mixed infection were fairly severe, like as vein clearing, leaf curling, leaf mottling, leaf mosaic, and yellow streaking. Mixed infection with LMoV and LSV was also found in lily bulbs which have been stored under unfavorable environmental conditions. LMoV predominated in our tests, whereas spread of Lilyvirus X (LVX) was not found. The nucleotide sequences of coat protein (CP) region of seven isolates (4 LMoV, 2 LSV, and 1 CMV) were compared with the corresponding regions of LMoV (AJ564636), LSV (AJ516059) and CMV(AJ296154). The nucleotide sequence homologies between reference viruses and seven isolates were 95-99%. Complete sequencing of seven isolates is necessary to obtain more information on the molecular characteristics of these viruses as well as to increase sensitivity and rapidity of viral detection.

키워드

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