• Title/Summary/Keyword: quarantine

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Development of an RT-PCR assay and its positive clone for plant quarantine inspection of American plum line pattern virus in Korea

  • Da-Som Lee;Junghwa Lee;Seong-Jin Lee;Seungmo Lim;Jaeyong Chun
    • Korean Journal of Agricultural Science
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    • v.49 no.4
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    • pp.821-831
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    • 2022
  • American plum line pattern virus (APLPV), a member of the genus Ilarvirus in the family Bromoviridae, is one of the plant quarantine pathogens in Korea. In this study, 15 candidate primer sets were designed and examined to develop a reverse transcription polymerase chain reaction (RT-PCR) assay for plant quarantine inspection of APLPV. Using APLPV-infected and healthy samples, the primer sets were assessed for APLPV detection. To confirm the occurrence of nonspecific reactions, six ilarviruses (Apple mosaic virus, Asparagus virus 2, Blueberry shock virus, Prune dwarf virus, Prunus necrotic ringspot virus, and Tobacco streak virus) and 10 target plants (Prunus mume, P. yedoensis, P. persica, P. armeniaca, P. dulcis, P. tomentosa, P. avium, P. glandulosa, P. salicina, and P. cerasifera) were examined. Finally, two primer sets were selected. These primer sets could generate the expected amplicons even with at least 1 ng of the total RNA template in concentration-dependent amplifications. In addition, a positive clone was developed for use as a positive control in the abovementioned RT-PCR assay.

The pests survey of paprika export complexes and packing house in Korea (우리나라 파프리카 수출단지 및 선과장의 병해충 조사)

  • Kim, Gi-Don;Lee, Siwon;Kang, Eun-Ha;Shin, Yong-Gil;Jeon, Jae-Yong;Heo, Noh-Yeol;Lee, Heung-Sik
    • Korean Journal of Agricultural Science
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    • v.40 no.2
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    • pp.93-99
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    • 2013
  • The disease and insect were surveyed locally in greenhouse, fruit packing house and store house of 51 farms in 13 towns having purpose of paprika exportation. By analysis, various disease and insect were not only founded locally but more ones detected in farms having old facilities and no natural enemy. We found 15 pathogens such as Fusarium spp., Alternaria solani, Leveilluila taurica, PepMV (Pepino mosaic virus) and TMV (Tobacco mosaic virus) in greenhouse, Fusarium spp. in fruit packing house and Penicillium spp. in store house. We found 15 insects in greenhouse such as Bemisia tabaci, rialeurodes vaporariorum and Myzus persicae in greenhouse, Hylobitelus haroldi in fruit packing house. However, the problem quarantine disease and insect for importation and exportation were not detected in inspection time.

A Study on the Improvement of Quarantine Act for Effective Quarantine System (효과적 검역체계를 위한 검역법 개선방안)

  • Lee, Yoon Hyeon;Kim, Myeong Seong;Lee, Jinhong
    • Health Policy and Management
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    • v.28 no.3
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    • pp.301-307
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    • 2018
  • The development of transport is being easily shared with people all over the world. It is necessary to appropriately and effectively revise the domestic quarantine law because the fatal infectious diseases are at risk of being easily shared. Today, Korea has an advanced quarantine system approved by World Health Organization, but it maintains partnerships with related ministries (Ministry of Foreign Affairs, Ministry of Justice, local medical institutions) and to introduce new medical technology (electronic quarantine) is important. And since the prevention of quarantine infectious diseases and prevention of the spread, in order to maintain international cooperation with the International Health Regulations, the quarantine law and the system should be amended and improved effectively and it is also a way to prepare for the outbreak of new quarantine infectious diseases. In the past, Korea has experienced great confusion during the past outbreak of swine flu and Middle East respiratory syndrome coronavirus. To prevent similar cases from recurring in the past, the revision of the quarantine law and the improvement of the system should be done to cope with the changing environment (new infections, increased number of overseas travelers, etc.).

Experimental Infection with Cheolwon/Incheon Isolates of Classical Swine Fever Virus in Pig: Clinical Signs and Pathology

  • Kim, Jae-hoon;Kang, Kyung-il;Roh, In-soon;Kim, Heui-jin;Jean, Young-hwa;Cha, Sang-ho;Park, Eun-jin;Yoon, So-rah;Seok, Ju-myung;Song, Jae-Young;Kweon, Jun-Heon
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • 2003.10a
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    • pp.12-12
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    • 2003
  • Several cases of classical swine fever (CSF) were reoccurred in Cheolwon province and Incheon city in 2002. Two isolates (Cheolwon and Incheon) of classical swine fever viruses (CSFV) were successfully isolated and classified into genotype-2 with a phylogenetic analysis [l, 2]. (omitted)

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Novel reassortants of clade 2.3.4.4 H5N6 highly pathogenic avian influenza viruses possessing genetic heterogeneity in South Korea in late 2017

  • Lee, Yu-Na;Cheon, Sun-Ha;Kye, Soo-Jeong;Lee, Eun-Kyoung;Sagong, Mingeun;Heo, Gyeong-Beom;Kang, Yong-Myung;Cho, Hyun-Kyu;Kim, Yong-Joo;Kang, Hyun-Mi;Lee, Myoung-Heon;Lee, Youn-Jeong
    • Journal of Veterinary Science
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    • v.19 no.6
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    • pp.850-854
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    • 2018
  • Novel H5N6 highly pathogenic avian influenza viruses (HPAIVs) were isolated from duck farms and migratory bird habitats in South Korea in November to December 2017. Genetic analysis demonstrated that at least two genotypes of H5N6 were generated through reassortment between clade 2.3.4.4 H5N8 HPAIVs and Eurasian low pathogenic avian influenza virus in migratory birds in late 2017, suggesting frequent reassortment of clade 2.3.4.4 H5 HPAIVs and highlighting the need for systematic surveillance in Eurasian breeding grounds.

Salmonella enterica serotype Choleraesuis infection in weaned pigs: a first clinicopathological case report from Korea

  • Kim, Jongho;Kim, Gyeongyeob;Lee, Hyunkyoung;Moon, Bo-Youn;Lee, Kichan;Byun, Jae-Won;Park, Ji-Young;Lee, Kyoung-Ki;Jeoung, Hye-Young;Ko, Mi-Kyeong;Ku, Bok-Kyung;Chung, Yun Soo;Bae, You-Chan
    • Korean Journal of Veterinary Research
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    • v.62 no.2
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    • pp.14.1-14.5
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    • 2022
  • Salmonella enterica serotype Choleraesuis causes swine paratyphoid, with clinical findings of enterocolitis and septicemia. However, the clinicopathological features of S. Choleraesuis infections in pigs have not been reported in Korea. We describe the pathological findings of two weaned pigs with S. Choleraesuis infections, presenting with diarrhea, cough, and sudden death. Pathological examination indicated severe necrotic colitis in pig 1 and septicemic lesions in pig 2. Multidrug-resistant S. Choleraesuis was isolated from the pigs' lungs and intestinal contents. Further research is required for the surveillance of S. Choleraesuis infections in pigs and the virulence estimation in the S. Choleraesuis isolates.

Congenital systemic melanosis in a black mongrel pig (흑돼지에서의 선천성 전신 멜라닌증 발생 증례)

  • Jeong, Yea-Ji;Jung, Ji-Youl;Her, Ji-Woong;Baek, Kang-Hyun;Lee, Jong-Hyeong;Lee, Myoung-Heon;Yoon, Soon-Seek
    • Korean Journal of Veterinary Research
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    • v.55 no.2
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    • pp.145-148
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    • 2015
  • Four 3-day-old piglets with retarded growth were submitted to the Animal and Plant Quarantine Agency for diagnosis. Necropsy showed that one piglet had black spots ranging from 2 mm to 1 cm in diameter in the cerebellum, lungs, regional lymph nodes, and cecum. Histological findings were consistent with the gross appearance in which melanin pigmentation was observed in the organs mentioned above. Based on Fontana-Masson staining, we diagnosed this animal with systemic melanosis. To the best of our knowledge, this is the first case of systemic melanosis in black breeds of swine in Korea.

Identification of Three Fungi Newly Intercepted from Importing Plants in Korea

  • Hyun, Ik-Hwa;Heo, Noh-Yeoul;Chang, Seo-Yeon;Heo, Jong-Young;Mel'nik, Vadim
    • Mycobiology
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    • v.33 no.4
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    • pp.243-244
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    • 2005
  • Three fungi newly intercepted from importing plants were identified in 2004. They were Ascochyta chrysanthemi on Lactuca sativa from China, A. spinaciicola on Spinacia oleracea from Denmark, and Leptosphaerulina australis on Brassica oleracea var. capitata from China. The characters of these fungi were described and illustrated.

Postweaning Multisystemic Wasting Syndrome - Case studies

  • Kim, Jae-Hoon;Roh, In-Soon;Hwang, Eui-Kyung;Woo, Gyu-Hywong;Jean, Young-Hwa;Sohn, Hyun-Joo;Yoon, Kyoung-Jin;Janke, Bruce H.;Park, Choi-Kyu;Kim, Ki-Seuk
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • 2000.09a
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    • pp.28-28
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    • 2000
  • Postweaning Multisystemic Wasting Syndrome (PMWS) is a recently described disease of growing pigs characterized by progressive weight loss and chronic pneumonia. PMWS was first identified in western Canada in 1995, and has now been diagnosed in the US, Europe and Asia. Since 1999, 57 pigs from 23 farms of PMWS have been diagnosed in the National Veterinary Research and Quarantine Service (NVRQS). The most common age of onest was clustered around 6-week age. (omitted)

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A Newly Known Genus Charitoprepes Warren (Lepidoptera: Pyraloidea: Crambidae) in Korea, with Report of C. lubricosa Warren (한국산 Charitoprepes 속 (나비목: 명나방상과: 포충나방과)의 1 미기록종 보고)

  • Kim, Minyoung;Park, Young-Mi;Hyun, Ik-Hwa;Kang, Byoung-Hyo;Oh, Si-Heon;Jwa, Jae-Kwang;Hyun, Young-Kwon;Lee, Heung-Sik
    • Korean journal of applied entomology
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    • v.53 no.3
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    • pp.301-303
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    • 2014
  • The genus Charitoprepes (Warren), a probable vagrant group of the family Crambidae is newly recorded for the first time from the Korean Peninsula, which was described based on C. lubricosa (Warren) from Jeju islands. Diagnosis and illustrations of detailed diagnostic characters, including genitalia are provided.