The Manufacture of Antioxidant Filter and Removal Efficiency of the Free Radicals in Mainstream Smoke

항산화 필터 제조 및 주류연중 free radicals 제거 효과

  • Received : 2009.06.12
  • Published : 2009.06.25

Abstract

This study describes the manufacturing method of antioxidant filter and evaluate the reduction of free radical and biological activity from mainstream smoke. When we used spray-brush for the application of antioxidant solution to the filter, cv(coefficient of variation) levels of antioxidant added to filter ranged within 3 %, which means stable and constant feeding process. 0.3~0.5mg per cigarette of antioxidant were sprayed to acetate and active carbon part in the filter plug, respectively. It was considered that the use of antioxidant added filter resulted in the reduction of the gaseous compounds of free radicals from 17 % to 19 % in the mainstream smoke. Variation for removal efficiency of free radical in antioxidant filter added to acetate part was more pronounced than that of filter added to active carbon part. Smoking deliveries of tar, nicotine for the antioxidant added filters were similar when comparing to the non-antioxidant added filter, but the delivering amounts of isoprene were significantly reduced. The estimation of consumed GSH(glutathion) showed that the gaseous toxicity of the antioxidant added filter was 14 % ~ 23 % lower than that of non-antioxidant added filter. No significant differences were observed for the total taste quality in sensory evaluation.

Keywords

References

  1. Baum, S.L., Anderson, G.M., Baker, R.R., Murphy, D.M. and Rowlands, C.C. (2003) Electron spin resonance and spin trap investigation of free radicals in cigarette smoke: development of a quantification procedure, Analytica Chimica Acta. 481: 1-13 https://doi.org/10.1016/S0003-2670(03)00078-3
  2. Bluhm, A.C., Weinstein, J. and Sousa, J.A. (1971) Free radicals in tobacco smoke. Nature 229: 500 https://doi.org/10.1038/229500a0
  3. Cahours, X., Blanchet, M., Haond, C., Marchand V. and Dumery, B.A. (2006) Cell-free method to assess the gaseous phase cytotoxicity of cigarette smoke. Coresta Conference Paris, France
  4. Church, D.F. and Pryor, W.A. (1985) Free radical chemistry of cigarette smoke and its toxicological implications. Environ. Health Pespect. 64: 111-130 https://doi.org/10.2307/3430003
  5. Cristin, A., Daniela, G. and Alfredo, N. (2003) A review of in vitro methods to assess the biological activity of tobacco smoke with the aim of reducing the toxicity of smoke. Toxicol. In vitro 17: 587-594 https://doi.org/10.1016/S0887-2333(03)00091-2
  6. Lyons, M.J., Gibson, J.E. and Ingram, D.J.E. (1958) Free-Radicals produced in Cigarette Smoke, Nature 181: 1003-1004 https://doi.org/10.1038/1811003a0
  7. Müller, C. and Intorp, M. (2003) Free radicals in mainstream cigarette smoke : methodology and influencing parameters, CORESTA Congress Freiburg
  8. Park, C.H., Shon, H.O., Shin, H.J., Lee, H.S., Min, Y.K. and Hyun, H.C. (2007) Toxicity assessment of gas phase cigarette smoke using cell-free method. J. Kor. Soc. Tob. Sci. 29: 110-117
  9. Park, J.W., Kim, J.Y., Lim, S.H., Kim, C.R., Rhee, M.S. and Kim, Y.H. (2007) 주류연중자유 라디칼 제거용 아스코르빈산 유도체 함유 궐련 필터, 대한민국 특허, 10-0903951
  10. Park, J.W., Kim, S.H., Kim, J.Y., Kim C.R. and Rhee, M.S. (2007) The scavenger effects of various antioxidants in cigarette filters on the free radicals in mainstream smoke, 61ST Tobacco Science Research Conference, Charlotte, North Carolina, USA
  11. Pryor W. A, Tamura M, and Church D. F. (1984) ESR spin trapping study of radicals produced in NOx/olefin reactions: a mechanism for the production of he apparently long-lived radicals in the gas phase cigarette smoke. J Am Chem Soc., 106: 5073–5079 https://doi.org/10.1021/ja00330a006
  12. Pryor, W.A., Prier, D.G. and Church, D.F. (1983) Electron-spin resonance study of mainstream and sidestream cigarette smoke: Nature of the free radicals in gas-phase smoke and in cigarette tar. Environ. Health Perspect. 47: 345-355 https://doi.org/10.2307/3429523
  13. Takanami, Y., Takahashi, H. and Shibagaki, M. (2002) Studies on free radicals formation in vapour phase cigarette smoke, Proceedings of the Paper Presented at the 56th Tobacco Science Research Conference, Lexington, KY, USA,, Programme Booklet and Abstracts(abstract no. 74)
  14. Takebayashi, J., Tai, A. and Yamamoto, I. (2002) Long-Term Radical Scavenging Activity of ascorbic acid derivative against 1,1-Diphenyl-2-picrylhydrazyl, Biol. Pharm. Bull. 25(11): 1503-1505 https://doi.org/10.1248/bpb.25.1503
  15. Yamamoto, I., Tai, A., Fujinami, Y., Sasaki, K. and Okazaki, S. (2002) Synthesis and Characterization of a Series of Novel Monoacylated Ascorbic Acid Derivatives, 6-O-Acyl-2-O-r-D-glucopyranosyl-L-ascor bic Acids, as Skin Antioxidants, J. Med. Chem., 45: 462-468 https://doi.org/10.1021/jm010379f
  16. Zhao, B., Rohdewald, Peter and Li, J. (2002) Cigarette filter with Scavenging Effect on Free Radical in Cigarette Smoke and Its Preparation method, US Patent, 0074009 A1
  17. Zhao, B. L. (1989) Cigarette free radicals and cancer. Nature, 12:453-460