Journal of Adhesion and Interface (접착 및 계면)

The Society of Adhesion and Interface, Korea (한국접착및계면학회)
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In vitro Cytotoxicity Evaluation of Polydimethylsiloxane as a Biosensor Coating Material

바이오센서 코팅용 Polydimethylsiloxane의 생체외 세포독성 평가

  • Park, Subeom (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Lee, Jonghwan (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Na, Kyunga (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Jung, Jaeyeon (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Kim, Myungjin (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Park, Sungjae (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University) ;
  • Hyun, Jinho (Department of Biosystems and Biomaterials Science and Engineering, Seoul National University)
  • 박수범 (서울대학교 바이오시스템소재학부) ;
  • 이종환 (서울대학교 바이오시스템소재학부) ;
  • 나경아 (서울대학교 바이오시스템소재학부) ;
  • 정재연 (서울대학교 바이오시스템소재학부) ;
  • 김명진 (서울대학교 바이오시스템소재학부) ;
  • 박성재 (서울대학교 바이오시스템소재학부) ;
  • 현진호 (서울대학교 바이오시스템소재학부)
  • Received : 2009.05.15
  • Accepted : 2009.06.02
  • Published : 2009.06.30
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Abstract

PDMS was selected for a coating material of implantable biosensors and the cytotoxicity of extracts released from a polymer was evaluated using ISO 10993-5, Biological evaluation of medical devices-Part 5: Tests for in vitro cytotoxicity. Organo-tin was used as a positive control and a medium without serum was used as a negative control. Materials extract were prepared by incubating specimens in RPMI medium without serum ($125{\mu}L/cm^2$) for 24 h, 1 week and 6 weeks at $38^{\circ}C$. The evaluation of cytotoxicity was performed by two different methods : 1) seeding cells with extracts at the beginning 2) incubating extracts with cell sheets already formed on the plate. Both cell morphology and MTT numerical data were shown for the confirmation of cytotoxicity and cell spreading on the surface of PDMS.

생체적용 센서 코팅 재료로서 polydimethylsiloxane (PDMS)를 선정하였으며 합성 및 성형과정에서 용출될 수 있는 잔류 독성 물질의 세포독성을 확인하고자 하였다. ISO 10993-5, Biological evaluation of medical devices-Part 5 : Tests for in vitro cytotoxicity (의료기기의 생물 안정성 평가-제5부: 세포 독성 시험-체외시험)를 통하여 세포 독성 평가를 실시하였다. 양성 대조군으로 organo-tin을 사용하였으며 음성 대조군으로 혈청이 포함되지 않은 RPMI 1640배지를 사용하였다. 고체 시료의 표면적에 대하여 $125{\mu}L/cm^2$가 되도록 혈청이 포함되지 않은 RPMI 1640 배지를 용기에 첨가하였으며 $38^{\circ}C$를 유지하며 일정시간 동안 추출하였다. 세포 독성 평가는 1) NIH 3T3 fibroblast 단일세포층을 형성한 후 추출물을 첨가하는 방법과 2) 세포와 함께 추출물을 넣어 배양하는 방법을 동시에 시행하였다. 세포 형태학적인 변화 관찰과 MTT (tetrazolium dye, 2-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide) 시험법에 의한 세포 활성 측정을 병행함으로써 고체 시료로부터 추출된 물질의 세포독성 여부와 고체시료의 표면에 대한 세포의 감응성도 함께 관찰할 수 있었다.

Keywords

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