Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains

  • Zhang, C.X. (The Institute of Botany, Chinese Academy of Sciences) ;
  • Zhao, X. (The Institute of Botany, Chinese Academy of Sciences) ;
  • Han, F. (The Institute of Botany, Chinese Academy of Sciences) ;
  • Yang, M.F. (The Institute of Botany, Chinese Academy of Sciences) ;
  • Chen, H. (The Institute of Botany, Chinese Academy of Sciences) ;
  • Chida, T. (Chemicals Agrochemicals Planning and Development Department) ;
  • Shen, S.H. (The Institute of Botany, Chinese Academy of Sciences)
  • Published : 2009.04.30
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Abstract

Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB-1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-l, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.

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References

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