Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Characterization of polysaccharide A-1 from Opuntia ficus-indica and it's protection effect on alcoholic induced hepatic oxidative stress

Opuntia ficus-indica 다당 A-1의 특성 및 알코올유도 간 산화스트레스의 보호 효과

  • Ryu, Il-Hwan (College of Life Science and Natural Resources, Wonkwang University) ;
  • Kwon, Ji-Wung (College of Life Science and Natural Resources, Wonkwang University) ;
  • Lee, Eoh-Jin (College of Life Science and Natural Resources, Wonkwang University) ;
  • Yun, Young-Gab (Department of Oriental medical prescription, Wonkwang University) ;
  • Kwon, Tae-Oh (College of Life Science and Natural Resources, Wonkwang University)
  • 류일환 (원광대학교 생명자원과학대학) ;
  • 권지웅 (원광대학교 생명자원과학대학) ;
  • 이어진 (원광대학교 생명자원과학대학) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실) ;
  • 권태오 (원광대학교 생명자원과학대학)
  • Published : 2009.12.30
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Abstract

Reactive oxygen species(ROS) can induce hepatotoxicity and trigger apoptosis in the liver. In this study, we investigated the sulfated polysaccharide A-1 from Opuntia ficus-indica against alcoholic oxidative stress in human liver Hep G2 cell. An antioxidant substance A-1 obtained from the enzymatic extract of Opuntia ficus-indica fruit was purified by DEAE-cellulose ion exchange and sephadex G-100 gel permeation chromatography. The purification yield and molecular weight were 14.3% and 1.8 KDa, respectively. The A-1 predominately contained arabinose, galactose, rhamnose and also sulfate group. The structure of A-1 was investigated by periodate oxidation, FT-IR spectroscopy, $^1H$-NMR spectroscopy. The A-1 mainly composed of alternating unit of ${\rightarrow}4$)-$\alpha$-L- Rapp-2-$SO_3^-$-$\alpha$-L-Galp-($1{\rightarrow}$ and branched linkage of $\beta$-D-Arbp- ($5{\rightarrow}$. The antioxidative activity was measured using the SOD, CAT activity and GSH assay, respectively. The expression of Nrf2 protein was analyzed by western blotting. The viable cell count analyzed by autofluorescence. Oxidative stress induced by ethanol(1 M) were dramatically reduced by A-1 treatment. A-1 also prevented cell death induced by oxidative stress. It also increased expression Nrf2 protein level. We concluded that sulfated polysaccharide A-1 from Opuntia ficus-indica effectively protect Hep G2 liver cell from alcoholic oxidative stress.

Keywords

References

  1. Lee HC, Lee HS, Jung SH, Yi SY, Jung HK, Yoon JH and Kim CY. Association between polymorphism of ethanol-metabolizing enzyme and susceptibility to alcoholic cirrhosis in korean male population. J. Korean Med. Sci. 2001;16 :745-50 https://doi.org/10.3346/jkms.2001.16.6.745
  2. Crawford DW and Blankenhorn DH. Alterial wall oxygenation, oxyradicals and atherosclerosis. Atherosclerosis. 1991;89:97-108 https://doi.org/10.1016/0021-9150(91)90049-9
  3. Kaplowita N and Tsukamoto H. Oxidative stress and liver disease. Pro. liver Dis. 1996; 14:131-59.
  4. McCord JM. The evolution of free radical and oxidative stress. Am. J. med. 2000;108:652-659 https://doi.org/10.1016/S0002-9343(00)00412-5
  5. Lennon SV, Martin SJ and Cotter TG. Dose -dependent induction of apoptosis in human tumor cellline by widely diverging stimuli. Cell Prolif. 1991;24:203-14. https://doi.org/10.1111/j.1365-2184.1991.tb01150.x
  6. Sato N, Iwata S, Nakamura K, Hori T, Mori K and Yodoi J. Thiol-mediated redox regulation of apoptosis. J. Immunol. 1995;154:3194-203
  7. Lieber CS. Alcohol and the liver. update gastroenterology. 1994;106:1085-105. https://doi.org/10.1016/0016-5085(94)90772-2
  8. Seitz HK, Poschl G and Simanowski UA. Alcohol and cancer. Recent Dev. Alcohol. 1998;14:67-95.
  9. Nah HY, Lee WS, Joo YE, Kim HS, Choi SK, Rew JS, Kim SJ, Jung BW, Ahn BW, Park JS and Shin BA. Resverastol protects Hep G2 and Chang liver cell from oxidative stress. Chonam Med. J. 2005;41:243-52.
  10. Unnot D. Nutrition and metabolic effect of alcoholism :there relationship with alcoholic liver disease. Nutrition. 1999;15:583-9. https://doi.org/10.1016/S0899-9007(99)00090-8
  11. Shon MY, Kim TH and Sung NJ. Antioxidants and free radical scavenging activity of Phellinus baumii(Phellinus of Hymenochaetaceae) extracts. Food Chem. 2003;82:593-7. https://doi.org/10.1016/S0308-8146(03)00015-3
  12. Ramarathnam N, Osawa T, OchiH and Kawakishi S. The contribution of plant food antioxidants to human health. Trends in Food Science and Technology. 1995;6:75-82. https://doi.org/10.1016/S0924-2244(00)88967-0
  13. Wangensteen H, Samuelsen AB and Malterud KE. Antioxidant activity in extracts fromcoriander. Food Chemistry. 2004;88:293-7. https://doi.org/10.1016/j.foodchem.2004.01.047
  14. Kinsella JE, Frankel E, German B and Kanner J. Possible mechanisms for theprotective role of antioxidants in wine and plantfoods. Food Technology. 1993:85-9.
  15. Velioglu YS, Mazza G, Gao YL and Oomah BD. Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products. J. Agric. Food Chem. 1998:46:4113-7. https://doi.org/10.1021/jf9801973
  16. Cui H, Liu Q, Tao Y, Zhang H, Zhang L and DingK. Structure and chain conformation of a(1$\rightarrow$6)-$\alpha$-d-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative. Carbohydrate polymers. 2008;74:771-8. https://doi.org/10.1016/j.carbpol.2008.04.034
  17. Zou C, Du Y, Li Y, Yang J, Feng T, Zhang L and Kennedy JF. Preparation of lacquer polysaccharide sulfates and their antioxidant activity in vitro Carbohydrate polymers. 2008; 73:322-31. https://doi.org/10.1016/j.carbpol.2007.11.035
  18. Park EH, Kahng JH and Paek EA. Studies on the phamacological action of cactus: identification of its anti-inflammatory effct. Arch. Pharm. Res. 1998;21:30-4. https://doi.org/10.1007/BF03216749
  19. Jun HK, JeongYK and Ha BJ. Protective effect of Opuntia indica on glutathion and antioxidant enzyme. Kor. J. Life Sci. 2003;13 :911-6. https://doi.org/10.5352/JLS.2003.13.6.911
  20. Yu N, Zeng F and RaoL. Effect of polysaccharide from Opuntia dillenii Haw on acute liver injury in mice. Chinese journal of biochemical pharmaceutics. 2009;30:255-7.
  21. Trombetta D, Puglia C and Perri D Effect of polysaccharides from Opuntia ficus-indica(L.) cladodes on the healing of dermal wounds in the rat. Phytomedicine. inter. j. phytotherapy phytopharm. 2006;13:352-8. https://doi.org/10.1016/j.phymed.2005.06.006
  22. Alarcon-Aguilar FJ, Valdes-Arzate A and Xolalpa -Molina S. Hypoglycemic activity of two polysaccharides isolated from Opuntia ficus-indica and O.streptacantha. Proceedings of the Western Pharmacology Societ. 2003;46:139-42.
  23. Bo L, Wei XJ, Sun JL and Xu SY. Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed, Hizikiafusiforme. Carbohydratere search. intenet press. 2006
  24. Vieira RP, Mulloy B, Diniz JA and Moura PAS. Structure of a fucose-bran chedchondroitin sulfate from sea cucumber. J. Biol. Chem. 1991;266:13530-6.
  25. Sawardeker JS, Sloneker JH and Jeanes AR. Quantitative determination of monosaccharides as their alditol acetates by gas-liquid chromatography. Analyt. Chem. 1965;37:1602-4. https://doi.org/10.1021/ac60231a048
  26. Whistler RL. Iodimetric determination of amylose. In Gilbert, G.A. and Spragg, S.P.(ed.); Method in carbohydrate chemistry. New York :Academicpress. 1960:168-75
  27. Sedlak J and Lindsay RH. Estimation of total protein bound and non bound sulfhydryl group in tissue with Ellman's reagent. Anal.Biochem. 1968;25:192-6. https://doi.org/10.1016/0003-2697(68)90092-4
  28. Fridovich L. Biologic effect of the superoxide radical. Arch. Biochem. Biophys. 1986;247:1-15 https://doi.org/10.1016/0003-9861(86)90526-6
  29. Aubin JE. Autofluorescence of viable cultured mammalian cells. J. Histochem. Cytochem. 1979 ;27:36-43. https://doi.org/10.1177/27.1.220325
  30. Matauhiro B, Lillo LE, Saenz C, Urzur CC and Zarata O. Chemical characterization of the mucilage from of Opuntia indica. Carbohydrate Polymer. 2006;63:263-7. https://doi.org/10.1016/j.carbpol.2005.08.062
  31. Habibi Y, Mahrouz M, Marais MF, and Vignon MR. Carbohydrate Reserch. 2004;339:1201-5. https://doi.org/10.1016/j.carres.2004.02.004
  32. Habibi Y, Mahrouz M, Marais MF, and Vignon MR. Carbohydrate Reserch. 2004;339:1119-27. https://doi.org/10.1016/j.carres.2004.02.005
  33. Ruperez P, Ahrazem O and Leal JA. Potential antioxidant capacity of sulphated polysaccharides from edible brown seaweed, Fucus vesiculosus. J. Agri. Food Chem. 2002;50:840-5. https://doi.org/10.1021/jf010908o
  34. Mabeau S, Kloareg B, Joseleau JP. Fractionation and analysis of fucans from brown algae. Phytochemistry. 1990;29:2441-5. https://doi.org/10.1016/0031-9422(90)85163-A
  35. Xue Z, Xue CH and Li BF. Study of antioxidant activities of sulfated polysaccharides from Laminaria japonica. J. Appl. Phycol. 2007;19 :1-7. https://doi.org/10.1007/s10811-006-9103-1
  36. Ryu IH, Kim SS, Lee KS. Purification and Properties of Non-Cariogenicity Sugar Produced by Alkalophilic Bacillus sp. S-1013. J. Microbiol. Biotechnol. 2004;14:751-8.
  37. Lee SY, Ryu IH, Shim CS. purification and characterization of bioactivity compound acemannan from Aloevera. Kor. J. Phamacogn. 1997;28: 65-71.
  38. McAnally BH and Manna S.Determination of position of O-acethylgroupina $\beta$-(1-4) mannan from Aloe barbardensis miller. Carbohydr. Res. 1993;241:317-9. https://doi.org/10.1016/0008-6215(93)80122-U
  39. Andrei AS, Olga NY, Alexander SS, Vladimir MM and Nikolay EN. Synthesis of Neu5Ac and Neu5Gc- $\alpha$-(2-6')-lactodamine3-aminopropyl glycosdides. Carbohyd. Research. 2001;330:445-58 https://doi.org/10.1016/S0008-6215(01)00002-7
  40. Chaplin MF and Kennedy JFK. Carbohydrate analysis. The practical Approch series co. 1994 ;242-93
  41. Johanes VP, Lambertus D, Marinus D, Cornelis HH, Johnnis PK and Johannes FGV. Transfer of sialic acid in $\alpha$-2-6 linkage to monnose in Man$\beta$-4GlcNAc and Man$\beta$1-4GlcNAn by the action of Gal$\beta$1-4GlcNAca 2-6sialytransferase. FEB. 1989;256:179-84 https://doi.org/10.1016/0014-5793(89)81744-2
  42. MacMurry J. Organicchemistry. fifthed. Brooks/ Cole. 1999.
  43. Skoog DA, Holler FJ and Nieman TA. Principle of Instrumental analysis. fifthed. Harcourt Brace & Company. 1998;47-56
  44. Rosen DR. Mutation with familial amyotrophic letral sclerosis. Natuer. 1993;362:59-62. https://doi.org/10.1038/362059a0
  45. Katsuoka F, Motohashi H, Engel JD and Yamamoto M. Nrf2 transcriptional activities the mrfG gene through an antioxidant response element. J. Bio Chem. 2005;280:4483-90. https://doi.org/10.1074/jbc.M411451200