Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지

Effect of Lophatherum gracile on the mechanism of vasorelaxation in thoracic aorta

담죽엽 추출물의 혈관이완 기전에 대한 연구

  • Kim, Hye-Yoom (Professional Graduate School of Oriental Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Li, Xiang (Professional Graduate School of Oriental Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Yun-Jeong (Professional Graduate School of Oriental Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Seo, Hwan-Ho (Dept. of Acupuncture and Moxibustion, College of Oriental Medicine, Wonkwang University) ;
  • Cho, Nam-Geun (Dept. of Acupuncture and Moxibustion, College of Oriental Medicine, Wonkwang University) ;
  • Kang, Dae-Gill (Professional Graduate School of Oriental Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Ho-Sub (Professional Graduate School of Oriental Medicine, College of Oriental Medicine, Wonkwang University)
  • 김혜윰 (원광대학교 한의학전문대학원) ;
  • 리향 (원광대학교 한의학전문대학원) ;
  • 이윤정 (원광대학교 한의학전문대학원) ;
  • 서환호 (원광대학교 한의과대학 침구학교실) ;
  • 조남근 (원광대학교 한의과대학 침구학교실) ;
  • 강대길 (원광대학교 한의학전문대학원) ;
  • 이호섭 (원광대학교 한의학전문대학원)
  • Published : 2009.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

The vasorelaxant effect of an extract of Lophatherum gracile Brongn (ELB) and its possible action mechanism were ascertained in aortic tissues isolated from rats. ELB relaxed endothelium-intact thoracic aorta in a dose-dependent manner. However, the induced vascular relaxation was abolished by removal in endothelium of the thoracic aorta. Pretreatment of endothelium-intact vascular tissues with $N^G$-nitro-L-arginine methyl ester (L-NAME) or 1H-[1,2,4]-oxadiazole-[4,3-$\alpha$]-quinoxalin-1-one (ODQ) significantly inhibited vascular relaxation induced by ELB. Moreover, ELB significantly increased cGMP production in aortic tissues, which was blocked by pretreatment with L-NAME or ODQ. The vasorelaxant effect of ELB was attenuated by tetraethylammonium (TEA), and glibenclamide. ELB-induced vasorelaxation was not blocked by atropine, propranolol, indomethacin, verapamil, and diltiazem. Taken together, the present study demonstrates that ELB dilates vascular smooth muscle via an endothelium-dependent NO-cGMP signaling pathway, which may be at least in part related with the function of $K^+$ channels.

Keywords

References

  1. 신영기. 주치의와 환자가 함께 보는 교양서 고혈압. 서울:계축문화사. 2000:40.
  2. E Braunwald, Fauci Kasper, Hauser Longo, Jameson.국역 Harrison's principles of internal medicine. 서울:도서출판 MIP. 2003:1456-60.
  3. Major Disease and Health-related behaviors in Korea:An In depth analysis of 2001 National Health and Nutrition Examination survey. KIHASA, Seoul:Ministry of Health and Welfare. 2003:76.
  4. Martin N. Experimental cardiovascular depressant effect of garlic dialysate. J Ethnopharmacol. 1992;37:145-53. https://doi.org/10.1016/0378-8741(92)90072-Y
  5. Park PS, Sung NJ, Park MY. Effects of Dioscorea batatas and Gastrodiae rhizoma on lipid composition changes of liver, brain and kidney in rats. J East Asian Soc Dietary Life. 1999;9:188-94.
  6. Choi YJ, Park YS, Kim C, Chang YK. Evaluation of functional ability and nutritional risk according to self-rated health (SRH) of the elderly in Seouland Kyunggi-do. Kor J Nutr. 2004;37 :223-35.
  7. Kim NJ, Jung EA, Kim DH, Lee SI. Studies on the development of antihyperlipidemic drugs from Oriental Herbal Medicines (I)－Antihyperlipidemic activities of Oriental herbal medicines. Kor J Pharmacol. 1999;30:368-76.
  8. 이상인, 박선동. 한방임상처방학(韓方臨床處方 學): 방제구성의 이론과 실제, 영림사.1998: 361-84
  9. Levi R. Therapies for perioperative hypertension: Pharmacodynamic consideration. Acta Anaesthesiol Scand.1993;37:16-9.
  10. 권년수. 산화질소 생성의 생화학. 심혈관 질환의 기초의학 심포지움. 1995:41-5.
  11. Vanhoutte Paul M. Endothelium-dependent hyperpolarizations: the history. Pharmacol Res. 2004;49:503-8. https://doi.org/10.1016/j.phrs.2003.11.015
  12. 생약학교재편찬위원회. 생약학. 동명사.1998:47
  13. 김창민, 신민교, 안덕균, 이경순. 완벽 중약대사전. 정담.제2권, 1999:881-8.
  14. Kim SZ, Kim SH, Park JK, Kho GY, Cho KW. Presence and biological activity of C-type natriuretic peptide-dependent guanylate cyclase-coupled receptor in the penile corpus cavernosum. J Urol. 1998;159:1741-6. https://doi.org/10.1097/00005392-199805000-00104
  15. Furchgott RF, and Zawadzki JU. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature. 1980;288:373-6. https://doi.org/10.1038/288373a0
  16. Hau C, and David GH. Endothelial dysfunction in cardiovascular diseases; the role of oxidant stress. Circ Res. 2000;87:840-4. https://doi.org/10.1161/01.RES.87.10.840
  17. Rubanyi GM. Endothelium-derived relaxing and contracting factors. J Cell Biochem.1991;46: 27-36. https://doi.org/10.1002/jcb.240460106
  18. MacNaul KL, Hutchinson NI. Differential of iNOS and cNOS mRNA in human vascular smooth muscle cells and endothelial cells under normal and inflammatory conditions. Biochem Biophys Res Commun. 1993;196:1330-4 https://doi.org/10.1006/bbrc.1993.2398
  19. Meng W., Ma J, Ayata C, Hara H, Huang PL, Fishman MC, Moskowitz MA. Ach dilates pial arterioles in endothelial and neuronal NOS knockout mice by NO-dependent mechanisms. Am J Physiol. 1996;271:1145-50.
  20. Hutcheson IR, and Griffith TM. Central role of intracellular calcium stores in acute flowand agonist-evoked endothelial nitric oxide release. Br J Pharmacol. 1997;122:117-25. https://doi.org/10.1038/sj.bjp.0701340
  21. Sharma NR, and Davis MJ. Substance P-induced calcium entry in endothelial cells is secondary to depletion of intracelluar stores. Am J Physiol.1995;268:962-73.
  22. Guerrero MF, Puebla P, Carron R, Martin ML, San Roman, L.Quercetin 3, 7-dimethyl ether:a vasorelaxant flavonoid isolated from Croton schiedeanus Schlecht. J Pharm Pharmacol. 2002;54:1373-8. https://doi.org/10.1211/002235702760345455
  23. Lemos VS, Freitas MR, Muller B, Lino YD, Queiroga CE, CortesSF. Diocleinanew nitric oxide- and endothelium-dependent vasodilator flavonoid. Eur J Pharmacol. 1999;386:41-6. https://doi.org/10.1016/S0014-2999(99)00747-5
  24. Ko FN, Huang TF, Teng CM. Vasodilatory action mechanisms of apigenin isolated from Apium graveolens in rat thoracic aorta. Biochim Biophys Acta. 1991;1115:69-74. https://doi.org/10.1016/0304-4165(91)90013-7
  25. Loeb AL, Izzo NJ, Johnson RM, Garrison JC, Peach MJ. Endothelium-derived relaxing factor release associated with increased endothelial cell inositol triphosphate and intracellular calcium. Am J Cardiol. 1988;62:36-40
  26. Van Breemen C, Cauvin C, Johns A, Leijten P, Yamamoto H. Ca2+ regulation of vascular smooth muscle. Fed Proc. 1986;45:2746-51
  27. Okabe K, Kitamura K, Kuriyama H. Features of 4-aminopyridine sensitive outward current observed in single smooth muscle cells from the rabbit pulmonary artery. Pflugers Arch. 1987;409:561-8. https://doi.org/10.1007/BF00584654
  28. Nevala R, Paukku K, Korpela R, Vapaatalo H. Calcium-sensitive potassium channel inhibitors antagonize genistein-and daidzein-induced arterial relaxation in vitro. Life Sci. 2001;69:1407-17 https://doi.org/10.1016/S0024-3205(01)01233-4
  29. Furchgott RF. Studies on relaxation of rabbit aorta by sodium nitrite: the basis for the proposal that the acid-activatable inhibitory factor from retractor penis is inorga nicnitrite and the endothelium-derived relaxing factor is nitric oxide. In Vasodilation: Vascular Smooth Muscle, Peptides, and Endothelium (P.M.Vanhoutte, ed.), Raven Press, New York. 1988:401-14.