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Microbial Analysis and Safety Evaluation in the Process of Packaged Tofus

포장두부의 가공공정에서 미생물 분석 및 안전성 평가

  • Wang, Soun-Nam (Kim's Institute of Food Safety Co., Ltd.) ;
  • Choi, Sung-Won (Department of Food and Culinary Art, Osan University) ;
  • Hur, Nam-Yoon (Department of Food and Culinary Art, Osan University) ;
  • Baik, Moo-Yeol (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Lee, Han-Seung (Department of Bio-Food Materials, College of Medical and Life Sciences, Silla University) ;
  • Kim, Chang-Nam (Kim's Institute of Food Safety Co., Ltd.)
  • 왕순남 ((주)김창남식품안전연구소) ;
  • 최성원 (오산대학교 호텔조리계열) ;
  • 허남윤 (오산대학교 호텔조리계열) ;
  • 백무열 (경희대학교 식품공학과) ;
  • 이한승 (신라대학교 의생명과학대학 바이오식품소재학과) ;
  • 김창남 ((주)김창남식품안전연구소)
  • Published : 2009.04.30

Abstract

This study was conducted to investigate microbial distribution in the processing steps and to estimate quality index and shelf life of packaged Tofu (soybean curd). Sanitation and safety of Tofu were analysed in aspects of total viable counts (TVCs) and coliforms. Organoleptic deterioration was observed from some packaged Tofu when their TVCs were over $10^6\;CFU/g$. The controlled simply packaged Tofu and sterilized Tofu with TVCs of under $10^5\;CFU/g$ were 32.0% and 86.9% of the total samples, respectively. Also, the controlled simply packaged Tofu and sterilized Tofu with negative coliforms were 12.0% and 83.7% of the total samples, respectively. TVCs and coliforms increased in some processing steps, which include washing and soaking of raw soybeans, and formation and 1st cooling of packaged Tofu. Increases of TVCs and coliforms in the washing and soaking step were due to contamination from the soaking tank and airborne bacteria, whereas increases of TVCs and coliforms in the grinding step were due to contaminations from the grinder, line and reserving tank. TVCs and coliforms increased in the formation and 1st cooling step of packaged Tofu due to contaminations from filter wools, trays, employee's hands, cooling water, formed products and filter wools.

본 연구는 생산 후 3일 이내인 시판두부의 가공공장별, 두부유형별 위생실태를 파악하여 두부에 대한 미생물 규격의 설정 가능성을 검토하며, 가공단계별 총생균수와 대장균군을 분석하여 오염 원인을 규명코자 실시되었다. 시판중인 두부의 위생성은 동일유형이더라도 가공공장마다 차이가 있었고 동일공장의 시료 간에도 차이를 보였다. 총생균수가 $1.0{\times}10^6CFU/g$ 이상을 보인 일부 포장두부에서는 관능적 부패 변질 현상이 관찰되었다. 총 148개 두부시료 중 총생균수와 대장균군이 각각 $1.0{\times}10^5CFU/g$ 이하와 음성에 적합한 시료는 단순포장두부가 32.0%와 12.0%, 포장가열두부가 86.9%와 83.7%인 것으로 조사되었다. 이러한 가공공장에 따른 위생성 차이는 가열과 열처리사이의 공정인 응고, 압착, 성형, 1차냉각, 내포장공정에서 2차오염 정도의 차이에서 기인하는 것으로 생각되었다. 포장두부의 가공공정 중 미생물이 증가하여 각별한 관리가 요구되는 공정으로는 원료대두의 세척 침지와 마쇄, 응고 후 압착 성형, 1차냉각공정이었다. 따라서 보다 위생적인 두부를 가공하기 위해서는 이들 가공공정에서 사용되는 각종 설비나 작업도구, 성형포 및 작업자 등에 대한 세척 및 소독 강화가 절실하게 요구되었다.

Keywords

References

  1. Chun, K. H., B. Y. Kim, T. I. Son, and Y. T. Hahm. 1997. The extension of Tofu shelf-life with water-soluble degraded chitosan as immersion solution. Korean J. Food Sci. Technol. 29, 476-481
  2. Chun, K. H., B. Y. Kim, T. I. Son, and Y. T. Halm. 1999. Extension of Tofu shelf-life with water soluble degraded chitosan as coagulant. Korean J. Food Sci. Nutr. 28, 161-166
  3. Harrigan, W. F. and M. E. McCance. 1976. Laboartory methods in food and dairy microbiology. Academic Press Inc., New York, pp.231-236, 361
  4. Imamura, Y., H. Kanayama, and Y. Toda. 1998. Recycling treatment for the manufacturing drain of soybean food (Tofu). Inorganic materials 5, 45-53
  5. Jang, W. Y., B. Y. Kim, and M. H. Kim. 1995. Prediction of the rheological of soybean curd during storage by using WLF equation. J. Korean Soc. Food Sci. Nutr. 27, 193-198
  6. Kim, C. N., S. J. Chun, W. T. Oh, S. H. Park, and H. C. Yu. 1996. Korea health industry development report for the studies on the application of HACCP to food industry. pp. 35-37
  7. Kim, H. J., B. Y. Kim, and M. H. Kim. 1995. Rheological studies of the Tofu upon the processing conditions. Korean J. Food Sci. Technol. 27, 324-328
  8. Kim, J. S. and S. Y. Choi. 2008. Quality characteristics of soybean curd with omija extract. Korean J. Food & Nutr. 21, 43-50
  9. Lee, Y. W., J. Y. Jung, S. G. Park, and S. W. Kim. 1997. Normal flora and effect of storage temperature and period in the commercial fish and shellfish. J. Food Hyg. Safety 12, 20-25
  10. Park, K. N., L. Y. Park, D. G. Kim. G. S. Park, and S. H. Kim. 2007. Effect of turmeric (Curcuma aromatica salab.) on shelf life of Tofu. Korean J. Food Preserv. 14, 136-141
  11. Park, W. H. and S. H. YI. 2003. The application of HACCP system to soybean curd and its effectiveness. J. Food Hyg. Safety 18, 202-210
  12. Roh, W. S. 1998. Microbial analysis of processing and evaluation of shelf life of fried bean curd. J. Food Hyg. Safety 13, 62-67
  13. Shin, H. Y., K. J. Ku, S. K. Park, and K. B. Song. 2006. Use of freshness Indicator for determination of freshness and quality change of Tofu during storage. J. Korean Soc. Appl. Biol. Chem. 49, 158-162
  14. Solberg, N., J. J. Burkalew, C. M. Chen, D. W. Schaffner, K. O'Neil, J. McDowell, L. S. Post, and M. Boderck. 1990. Microbiological safety assurance system for food service facilities. Food Technol. 44, 68-73
  15. Stringer, M. F. 1994. Safety and quality management through HACCP and ISO 9000. Dairy, Food and Environment, Sanitation 14, 428-481
  16. Woo, I. T., K. N. Park, and S. H. Lee. 2007. Antimicrobail activity of Scutellaria baicalensis georgi against various pathogens and spoilage bacteria isolated from Tofu. J. Korean Soc. Food Sci. Nutr. 36, 470-475 https://doi.org/10.3746/jkfn.2007.36.4.470