한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제13권4호
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  • Pages.341-351
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  • 2009
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

대하(Fenneropenaeus chinensis)의 난소 성숙에 대한 Serotonin, Ecdysone 그리고 HCG의 효과

Effects of Serotonin, Ecdysone and HCG on Ovarian Maturation of the Fleshy Prawn, Fenneropenaeus chinensis

  • 발행 : 2009.12.31
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초록

대하, Fenneropenaeus chinensis의 난소 성숙에 대한 serotonin(5-hydroxytryptamine, 5-HT), ecdysone(20-hydroxyecdysone, 20-E) 그리고 HCG(human chorionic gonadotropin)의 효과를 조사하였다. 대하에 5-HT(체중 g당 $5{\mu}g,\;20{\mu}g$), 20-E(체중g당 $5{\mu}g,\;10{\mu}g,\;20{\mu}g$) 그리고 HCG(체중 g당 5 IU, 10 IU)를 5일 간격으로 3회 주사한 후 10일이 더 지난 시점에 채집하여 성 성숙도지수(GSI)의 변화와 난소의 조직학적 변화를 조사하였다. 호르몬제의 효과를 비교하기 위해 멸균한 생리식염 수만을 주사한 대조구와 아무 것도 처리하지 않은 무처리구를 설정하였다. 20-E와 HCG를 처리한 실험구에서는 난소 성숙 효과가 전혀 나타나지 않았으나, 이른 봄에 5-HT를 체중 g당 $20{\mu}g$로 처리한 실험구에서는 유의한 난소 성숙효과를 보였다. 본 실험 결과에서 대하에 5-HT를 체중 g당 $20{\mu}g$ 이상 처리하면 난소성숙을 유도할 수 있다는 것을 보여주었으며, 이는 대하의 종묘 생산에 유용하게 사용될 수 있을 것이다.

The effects of serotonin (5-hydroxytryptamine, 5-HT), ecdysone (20-hydroxyecdysone, 20-E) and HCG (human chorionic gonadotropin) on the ovarian maturation of the fleshy shrimp, Fenneropenaeus chinensis were investigated. Adult female fleshy shrimps were injected with 5-HT ($5{\mu}g$/g body weight (BW) or $20{\mu}g$/g BW), 20-E ($5{\mu}g$/g BW, $10{\mu}g$/g BW or $20{\mu}g$/g BW) or HCG (5 IU/g BW or 10 IU/g BW) three times at 5-day intervals and were sacrificed 10 days after the last injection. The effects were compared against an intact control group and sham control group which received the injection of sterile saline solution as a vehicle. 20-E and HCG showed no effectiveness on ovarian maturation in fleshy shrimps. However flesh shrimp given 5-HT of $20{\mu}g$/g BW showed significant increase in gonadosomatic index (GSI) in early spring. The results suggest that treatment of 5-HT of $20{\mu}g$/g BW or more is useful approach for the nursery production of the fleshy shrimp, Fenneropenaeus chinensis aquaculture.

키워드

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