The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Stability Study for Herbal Drug According to Storage Conditions and Periods

탕약의 실온과 냉장보관 및 기간별 안정성에 대한 실험적 연구

  • Son, Jin-Young (Internal Medicine & Immune Center, Daejeon University) ;
  • Shin, Jang-Woo (Internal Medicine & Immune Center, Daejeon University) ;
  • Son, Chang-Gue (Internal Medicine & Immune Center, Daejeon University)
  • 손진영 (대전대학교 한의과대학 간장면역학 교실) ;
  • 신장우 (대전대학교 한의과대학 간장면역학 교실) ;
  • 손창규 (대전대학교 한의과대학 간장면역학 교실)
  • Published : 2009.03.31
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Abstract

Objectives: This study aimed to investigate the stability of a decoction using three herbal plants and their major components according to the storage conditions and periods. Materials and Methods: A three-herb mixture (1:1:1) of Glycyrrhiza uralensis Fischer, Artemisia capillaris Thunberg, and Poncirus trifoliata Rafinesqui was decocted and kept at room temperature ($25{\pm}2^{\circ}C$) or cold temperature ($4^{\circ}C$) for 0, 2, 4 or 8 weeks in liquid form in a plastic pack under dark conditions. At time points given, they were lyophilized. 200 mg of powdered samples were dissolved in 1 mL of 90% methanol and then applied to a high performance thin layer chromatography (HPTLC) with glycyrrhizin, 6,7-dimethoxycoumarin or poncirin for quantitative or qualitative analysis. Results: There were no gross changes in HPTLC-based compositional band-patterns of the three herbal mixture according to the storage conditions and period. The major components of each herb, glycyrrhizin, 6,7-dimethoxycoumarin and poncirin, showed slight time-dependent reduction in their contents both at room and cold temperature for 8 weeks. Conclusion: We could conclude that the current herbal decoction is generally safe for the stability at both RT or CT for at least 8 weeks. Nevertheless, we proposed that further advanced studies are required for more multiple herbal mixtures and longer storage periods.

Keywords

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