The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Preventive Effects of Peony Root Extracts on Oxidative Stress, Thrombosis and Atherosclerosis

백작약 추출물이 항산화활성, LDL 산화 억제 및 혈전용해에 미치는 영향

  • Park, Soon-Gi (Dept. of Diagnostics, Dongguk University) ;
  • Lee, Min-Ja (Cardiovascular Medical Research Center, Dongguk University) ;
  • Jung, Hyun-Jung (Cardiovascular Medical Research Center, Dongguk University) ;
  • Lee, Hye-Sook (Cardiovascular Medical Research Center, Dongguk University) ;
  • Kim, Hyuck (Cardiovascular Medical Research Center, Dongguk University) ;
  • Na, Sun-Taek (Dept. of Diagnostics, Dongguk University) ;
  • Park, Sun-Dong (Dept. of Diagnostics, Dongguk University) ;
  • Park, Won-Hwan (Cardiovascular Medical Research Center, Dongguk University)
  • 박순기 (동국대학교 한의과대학 진단학 교실) ;
  • 이민자 (동국대학교 심혈관질환 기초의과학센터) ;
  • 정현정 (동국대학교 심혈관질환 기초의과학센터) ;
  • 이혜숙 (동국대학교 심혈관질환 기초의과학센터) ;
  • 김혁 (동국대학교 심혈관질환 기초의과학센터) ;
  • 나선택 (동국대학교 한의과대학 진단학 교실) ;
  • 박선동 (동국대학교 한의과대학 진단학 교실) ;
  • 박원환 (동국대학교 심혈관질환 기초의과학센터)
  • Published : 2009.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: There is currently increased interest in the identification of natural antioxidant compounds derived from various plants. Peony Root (PR) is used worldwide for the treatment of many types of cardiovascular disease including atherosclerosis and hypertension. It has been used in Korean traditional medicine for the treatment of glycosuria, hypertension and cancer. However, to date, no studies concerning the antioxidant properties of PR have been conducted. Therefore, this study was conducted to evaluate the in vitro scavenging activity, inhibitory effect of LDL oxidation of pro-oxidant reactive species and anti-thrombosis effect in response to treatment with PR using various screening methods including biological and non-biological oxidants. Methods: In this study, the antioxidant activity of extract from PR was studied with in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$-induced human LDL oxidation and the inhibitory effect on collagen-induced platelet aggregation. Results: The PR extracts were found to have a potent scavenging activity of oxidative stress [DPPH, superoxide anion, hydroxyl radical, nitric oxide and peroxynitrite, etc.] as well as an inhibitory effect on LDL oxidation and on platelet aggregation. Conclusions: The PR extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drugs against oxidative stress and atherosclerosis.

Keywords

References

  1. Giles GI, Tasker KM, Jacob C. Hypothesis: the role of reactive sulfur species in oxidative stress. Free Radical Biology & Medicine. 2001;31:1279-83. https://doi.org/10.1016/S0891-5849(01)00710-9
  2. Fubini B, Hubbard A. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis. Free Radical Biology & Medicine. 2003; 34:1507-16. https://doi.org/10.1016/S0891-5849(03)00149-7
  3. Frank MS, Martijn K. Randomized clinical trials on the effects of dietary fat and carbohydrate on plasma lipoprotein and cardiovascular disease. The American Journal of Medicine. 2002;113:13-24. https://doi.org/10.1016/S0002-9343(01)00987-1
  4. Ross R. Atherosclerosis. An inflammatory disease. N. Engl. J. Med. 1999;340:115-26. https://doi.org/10.1056/NEJM199901143400207
  5. Libby P. Inflammation in atherosclerosis. Nature. 2002;420:868-74. https://doi.org/10.1038/nature01323
  6. Raya AA, Raya SA. Inflammation: A pivotal link between autoimmune diseases and atherosclerosis. Autoimmunity Reviews. 2006;5:331-37. https://doi.org/10.1016/j.autrev.2005.12.006
  7. Amarowicz R, Naczk M, Shahidi F. Antioxidant activity of various fractions of non-tannin phenolics of canola hulls. Journal of Agricultural and Food Chemistry. 2000;48:2755-59. https://doi.org/10.1021/jf9911601
  8. Verstraeta M, Lijnen HR, Collen D. Thrombolytic agents in development. Drugs. 1995;50:29-42. https://doi.org/10.2165/00003495-199550010-00003
  9. Fujita M, Hong K, Ito Y, Fujii R, Kariya K. Nishimuro S. Thrombolytic effect of nattokinase on a chemically induced thrombosis model in rat. Biol. Pharm. Bull. 1995;18: 1387-91. https://doi.org/10.1248/bpb.18.1387
  10. Hanbang Yakrihak Gyojae Pyeochanwiwonhoe. Hanbang Yakrihak. Seoul:Shin-il sang sa. 2006:133-4.
  11. Lee JM, Choi SW, Jo SH, Lee SJ. Effect of Seeds Extract of Paeonia Lactiflora on Antioxidative System and Lipid Peroxidation of Liver in Rats Fed High-Cholesterol Diet. The Korean Nutrition Society. 2003;36(8):793-800.
  12. Jeong HG, You HJ, Chang YS, Park SJ, Moon YH, Woo ER. Inhibitory Effects of Medicinal Herbs on Cytochrome P450 Drug Metabolizing Enzymes. Kor. J. Pharmacogn. 2002;33(1):35-41.
  13. Kim HJ, Ha SC, Choi SW. Inhibition of Tyrosinase and Lipoxygenase Activities by Resveratrol and Its Derivatives from Seeds of Paeonia lactiflora. Nutraceuticals and Food. 2002;7(4):447-50. https://doi.org/10.3746/jfn.2002.7.4.447
  14. Roberta RE, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine. 1999;26:1231-7. https://doi.org/10.1016/S0891-5849(98)00315-3
  15. Maria GA, Sonia PT, Celestino SB, Julian CRG. Evaluation of the antioxidant properties of fruits. Food Chemistry. 2004;84:13-8. https://doi.org/10.1016/S0308-8146(03)00160-2
  16. Pietta P, Simonetti P, Gardana C Mauri P. Trolox equivalent antioxidant capacity (TEAC) of Ginkgo biloba flavonol and Camellia sinensis catechin metabolites. Journal of Pharmaceutical and Biomedical Analysis. 2000;23:223-6. https://doi.org/10.1016/S0731-7085(00)00272-7
  17. Choi CH, Song ES, Kim JS, Kang MH. Antioxidative activites of Castanea crenata Flos. methanol extracts. Korean J. Food Sci. Technol. 2003;35: 1216-20.
  18. Kand YH, Park YK, Lee GD. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J. Food Sci. Technol. 1996;33:626-32.
  19. Bielski BHJ, Shiue GG, Bajuk S. Reduction of nitro blue tetrazolium by CO2- and O2- radicals. J. Phys. Chem. 1980;84:830-33. https://doi.org/10.1021/j100445a006
  20. Frankel EN, Huang SW, Aeschbach R. Antioxidant activity of green teas in different lipid systems. J Am Oil Chem Soc. 1997;74:1309-15. https://doi.org/10.1007/s11746-997-0062-8
  21. Antosiewicz J, Popinigis J, Wozniak M, Damiani E, Carloni P, Greci L. Effects of indolinic and quinolinic aminoxyls on protein and lipid peroxidation of rat liver microsomes. Free Radic Bio Med. 1995;18:913-7. https://doi.org/10.1016/0891-5849(94)00196-Q
  22. Wang H, Zhao M, Yang B, Jiang Y, Rao G. Identification of polyphenols in tobacco leaf and their antioxidant and antimicrobial activities. Food Chemistry. 2008;107(4):1399-1406. https://doi.org/10.1016/j.foodchem.2007.09.068
  23. Patel RP, McAndrew J, Sellak H, White CR, Jo H, Freeman BA, Darley-Usmar VM.. Biological aspects of reactive nitrogen species. Biochimica et Biophysica Acta. 1999;1411:385-400. https://doi.org/10.1016/S0005-2728(99)00028-6
  24. Pfeilschifter J, Eberhardt W, Hummel R, Kunz D, Muhl H, Nitsch D, Pluss C, Walker G. Therapeutic strategies for the inhibition of inducible nitric oxide synthase-potential for a novel class of anti-inflammatory agents. Cell Biol. Int., 1996 ;20:51-8. https://doi.org/10.1006/cbir.1996.0008
  25. Lee GD, Chand HG, Kim HK. Antioxidative and nitrite-scavenging activities of edible mushrooms. Korean J. Food Sci. Technol. 1997;29:432-6.
  26. Virag L, Szabo E, Gergely P, Szabo C. Peroxynitrite induced cytotoxicity: metabolism and opportunities for intervention. Toxocol Lett. 2003;140-141:113-24. https://doi.org/10.1016/S0378-4274(02)00508-8
  27. Rubbo H, O’Donnell V. Nitric oxide, peroxynitrite and lipoxygenase in atherogenesis: mechanistic insights. Toxicology. 2005;208:273-88. https://doi.org/10.1016/j.tox.2004.11.023
  28. Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot. 2003;91:179-94. https://doi.org/10.1093/aob/mcf118
  29. Delledonne M, Polverari A, Murgia I. The functions of nitric oxide mediated signalling and changes in gene expression during the hypersensitive response. Antioxid. Redox Signal. 2003;5:33-41. https://doi.org/10.1089/152308603321223522
  30. Herbette S, Lenne C, de Labrouhe DT. Transcripts of sunflower antioxidant scavengers of the SOD and GPX families accumulate differentially in response to downy mildew infection, phytohormones, reactive oxygen species, nitric oxide, protein kinase and phosphatase inhibitors. Physiol. Plant. 2003;119:418-28. https://doi.org/10.1034/j.1399-3054.2003.00186.x
  31. Matsuura E, Hughes GRV, Khamashta MA. Oxidation of LDL and its clinical implication. Autoimmunity Rev. 2008;7(7):558-66. https://doi.org/10.1016/j.autrev.2008.04.018
  32. Katsube T, Imawaka N, Kawano Ya, Yamazaki Y, Shiwaku K, Yamane Y. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chemistry. 2006;97(1):25-31. https://doi.org/10.1016/j.foodchem.2005.03.019
  33. Oliveira-Carvalho AL, Guimarães PR, Abreu PA, Dutra DLS, Junqueira-de-Azevedo ILM, Rodrigues CR, Ho PL, Castro HC, Zingali RB. Identification and characterization of a new member of snake venom thrombin inhibitors from Bothrops insularis using a proteomic approach. Toxicon. 2008;51(4):659-71. https://doi.org/10.1016/j.toxicon.2007.11.026
  34. Jones MN, Holt RG. Activation of plasminogen by Streptococcus mutans. Biochemical and Biophysical Research Communications. 2004;322(1): 37-41. https://doi.org/10.1016/j.bbrc.2004.07.077
  35. Matsubara K, Hori K, Matsuura Y, Miyazawa K. Purification and characterization of a fibrinolytic enzyme and identification of fibrinogen clotting enzyme in a marine green alga, Codium divaricatum Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2000;125(1):137-43. https://doi.org/10.1016/S0305-0491(99)00161-3
  36. Chudzinski-Tavassi AM, Kelen EM, Paula-Rosa AP, Loyau S, Sampaio CA, Bon C, Angles-Cano E. Fibrino(geno)lytic properties of purified hementerin, a metallo-proteinase from the leech Haementeria depressa, Thromb. Haemost. 1998;80: 155-60.
  37. Shahriyary L, Yazdanparast R. Inhibition of blood platelet adhesion, aggregation and secretion by Artemisia dracunculus leaves extracts. Journal of Ethnopharmacology. 2007;114(2):194-78. https://doi.org/10.1016/j.jep.2007.07.029
  38. Rao AK. Acquired disorders of platelet function. Platelets. 2007:1051-76.