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Establishment of Analytical Method of Prochloraz in Cabbage, Apple, Mandarin, Pepper and Hulled rice with GC-ECD

GC-ECD를 이용한 배추, 사과, 감귤, 고추, 현미 중 살균제 Prochloraz의 분석법 확립

  • Lee, Eun-Mi (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Hye-Ri (Department of Agricultural Biotechnology, Seoul National University) ;
  • Riu, Myoung-Joo (Department of Agricultural Biotechnology, Seoul National University) ;
  • Park, Hee-Won (Department of Agricultural Biotechnology, Seoul National University) ;
  • Na, Ye-Rim (Department of Agricultural Biotechnology, Seoul National University) ;
  • Song, Hyuk-Hwan (Department of Agricultural Biotechnology, Seoul National University) ;
  • Keum, Young-Soo (Department of Agricultural Biotechnology, Seoul National University) ;
  • Zhu, Youngzhe (Qingdao Agricultural University) ;
  • Kim, Jeong-Han (Department of Agricultural Biotechnology, Seoul National University)
  • 이은미 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 이혜리 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 류명주 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 박희원 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 나예림 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 송혁환 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 금영수 (서울대학교 농업생명과학대학 농생명공학부) ;
  • ;
  • 김정한 (서울대학교 농업생명과학대학 농생명공학부)
  • Published : 2009.12.30

Abstract

Analytical method for prochloraz in cabbage, apple, pepper, mandarin, and hulled rice was established by conversion it to 2,4,6-trichlorophenol (TCP). Crop samples were extracted with acetonitrile, and partitioned with dichloromethane. The sample extracts were hydrolyzed with pyridine hydrochloride in a vial by heating for 1 hour, and analyzed with GC-ECD after partitioning with dichloromethane. Method quantification limit (MQL) of prochloraz was 0.01 mg/kg. Recoveries at 0.1 mg/kg level was 105-113% while at 0.5 mg/kg level was 82-87%. In both of the cases CV was less than 10%. Through this procedure soxhlet extraction and refluxing apparatus of conventional method were discarded and simple solvent extraction and small vial were successfully employed, resulting in simple, rapid, economic and more precise method.

Keywords

References

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