Journal of Applied Biological Chemistry

  • 제52권4호
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  • Pages.221-225
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  • 2009
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  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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지치뿌리로부터 분리한 Acetylshikonin의 LDL 산화 저해활성과 FPTase 저해활성

Inhibitory Effect of Acetylshikonin from Roots of Lithospermum erythrorhizon on LDL Oxidation and FPTase Activity

  • 김금숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 정태숙 (한국생명공학연구원) ;
  • 권병목 (한국생명공학연구원) ;
  • 김영옥 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 송경식 (경북대학교 농업생명과학대학) ;
  • 백남인 (경희대학교 생명공학원)
  • Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jeong, Tae-Sook (Korea Research Institute of Bioscience & Biotechnology) ;
  • Kwon, Byoung-Mok (Korea Research Institute of Bioscience & Biotechnology) ;
  • Kim, Young-Ok (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cha, Seon-Woo (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Song, Kyung-Sik (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Bek, Nam-In (Graduate School of Biotechnology, Kyung Hee University)
  • 발행 : 2009.12.31
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초록

Lithospermum erythrorhizon has been known well as one of traditional medicine for fever reduction, detoxication, and blood circulation improvement. This study was carried out to isolate biological active compounds from roots of Lithospermum erythrorhizon and to investigate their low density lipoprotein (LDL) antioxidant and anticancer activities. The hexane extract of Lithospermi Radix has been separated on silica gel chromatography and a naphthoquinone pigment compound 1 has been isolated. The structure of the compound 1 has been identified by spectroscopic technique, including MS and NMR, as acetylshikonin (1). Acetylshikonin showed significantly inhibitory activity on $Cu^{2+}$-induced human LDL oxidation with $IC_{50}$ value of $8.8\;{\mu}M$ and obvious anticancer effect by inhibiting farnesyl:protein transferase (FPTase) activity with $IC_{50}$ value of $23\;{\mu}M$, which suggested that acetylshikonin might be useful for the treatment of atherosclerosis and cancer.

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