한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제42권6호
  • /
  • Pages.614-620
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  • 2009
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
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해조류(톳, 감태) 혼합물의 사료 내 첨가가 넙치의 선천성 면역과 질병저항성에 미치는 영향

Effects of Dietary Supplementation of Alga Mixtures (Hizikia fusiformis and Ecklonia cava) on Innate Immunity and Disease Resistance Against Edwardsiella tarda in Olive Flounder (Paralichthys olivaceus)

  • 김성삼 (제주대학교 해양의생명과학부) ;
  • 장지웅 (제주대학교 해양의생명과학부) ;
  • 송진우 (제주대학교 해양의생명과학부) ;
  • 임세진 (제주대학교 해양의생명과학부) ;
  • 정준범 (제주대학교 해양의생명과학부) ;
  • 이상민 (강릉원주대학교 해양생명공학부) ;
  • 김강웅 (국립수산과학원 양식관리과) ;
  • 손맹현 (국립수산과학원 양식관리과) ;
  • 이경준 (제주대학교 해양의생명과학부)
  • Kim, Sung-Sam (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Jang, Ji-Woong (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Song, Jin-Woo (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Lim, Se-Jin (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Jeong, Joon-Bum (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Lee, Sang-Min (Faculty of Marine Bioscience and Technology, Kangnung-Wonju National University) ;
  • Kim, Kang-Woong (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Son, Maeng-Hyun (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Lee, Kyeong-Jun (Faculty of Marine Biomedical Science, Jeju National University)
  • 발행 : 2009.12.31
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초록

We report non-specific immune responses and its disease resistance against Edwardsiella tarda by alga mixture (HE; Hizikia:Ecklonia) in olive flounder for the first time. Five isonitrogenous (44% crude protein) and isocaloric (17.1 MJ $kg^{-1}$) diets were formulated to have 0%, 2%, 4%, 6% and 8% of the alga mixture. One of five experimental diets was fed triplicate groups of fish (30 fish/group) to apparent satiation in a flow through system. After a two week feeding, blood was sampled at 3, 6, 12, 24 h after the last feeding for a kinetic measurement of nitroblue tetrazolium (NBT) activity and healthy fish with similar sizes in each tank were selected and injected with 1 mL of E. tarda suspension ($1.0\times10^7$ CFU/mL) to evaluate the disease resistance of the fish. Dietary supplementation of alga mixtures resulted in significantly higher non-specific immune responses compared with the fish fed the control diet. The cumulative mortality was significantly lower in the fish groups fed alga mixture containing diets than control group in the challenge test with E. tarda. Therefore, the results in this study indicate that dietary supplementation of Hizikia and Ecklonia mixtures enhance the non-specific immune responses and a disease resistance of olive flounder.

키워드

참고문헌

  1. Alcaide E, Herraiz S and Esteve C. 2006. Occurrence of Edwardsiella tarda in wild European eels Anguilla anguilla from Mediterranean Spain. Dis Aquat Org, 73, 77-81 https://doi.org/10.3354/dao073077
  2. Anderson DP and Siwicki AK. 1995. Basic haema- tology and serology for fish health programs. In: Diseases in Asian aquaculture Ⅱ. Shariff, M, JR Arthur and RP Subasinghe, eds. Asian Fisheries Society, Manila, Philippines pp. 185
  3. AOAC. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Arlington, Virginia pp. 1298
  4. Athukorala Y, Kim KN and Jeon YJ. 2006. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food and Chemical Toxicology 44, 1065-1074 https://doi.org/10.1016/j.fct.2006.01.011
  5. Bang JD, Chun SK, Park SI and Choi YJ. 1992. Studies on the biochemical and serological charac- teristics of Edwardsiella tarda isolated from cultured flounder, Paralichthys olivaceus. J Fish Pathol 5, 29-35
  6. Bu HJ, Ham YM, Kim JM, Lee SJ, Hyun JW and Lee NH. 2006. Elastase and hyaluronidase inhi- bition activities of phlorotannins isolated from Ecklonia cava. Kor J Pharmacogn 37, 92-96
  7. Cho KJ, Lee YS and Ryu BH. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. Bull Korean Fish Soc 23, 345- 352
  8. Clavijo AM, Conray G, Santander J and Aponte F. 2002. First report of E. tarda from tilapias in Venezuela. Bull Eur Assoc Fish Pathol 22, 280-282
  9. Folch J, Lee M and Sloane–Stanley GH. 1959. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497-509
  10. Garling DLJ and Wilson RP. 1976. Optimum dietary protein to energy ratio for channel catfish fingerlings, Ictalurus punctatus. J Nutr 106, 1368-1375
  11. Haung HL and Wang BG. 2004. Antioxidant capacity and lipophilic content seaweeds collected from the Quandao coastline. J Agric Food Chem 52, 4993-4997 https://doi.org/10.1021/jf049575w
  12. Hong JH, Son BS, Kim BK, Chee HY, Song KS, Lee BH, Shin HC and Lee KB. 2006. Antihypertensive effect of Ecklonia cava extract. Kor J Pharmacogn 37, 200-205
  13. Kim JA and Lee JM. 2004. The changes of biologically functional compounds and antioxidant activities in Ecklonia cava with blanching times. Korean J Food Culture 19, 369-377
  14. Kim SS and Lee KJ. 2008. Effects of dietary kelp (Ecklonia cava) on growth and innate immunity in juvenile olive flounder Paralichthys olivaceus (Temminck et Schlegel). Aquac res 39, 1687-1690
  15. Kim SS, Galaz GB and Lee KJ and Lee YD. 2006. Effects of dietary supplementation of Spirulina and Astaxanthin for juvenile olive flounder, Paralichthys olivaceus in low temperature season. J Aquaculture 19, 57-63
  16. Kim YY, Lee KW, Kim GB and Cho YJ. 2000. Studies on physiochemical and biological properties of depolymerized alginate from sea tangle, Laminaria japonicus by thermal decomposition. J Kor Fish Soc 33, 393-398 https://doi.org/10.3746/jkfn.2003.32.3.406
  17. Ko MS, Shin KM and Lee MY. 2002. Effects of Hizikia fusiforme ethanol extract on antioxidative enzymes in ethanol-induced hepatotoxicity of rat liver. J Korean Soc Food Sci Nutr 31, 87-91 https://doi.org/10.3746/jkfn.2002.31.1.087
  18. Kusuda R, Itami T, Mumekiyo M and Nakajima H. 1997. Characteristics of an Edwardsiella sp. from an epizootic of cultured crimson sea breams. Bull Jpn Soc Scient Fish 43, 129-134
  19. Larson RA. 1988. The antioxidants of higher plants. Phytochemistry 27, 969-978 https://doi.org/10.1016/0031-9422(88)80254-1
  20. Lee HA, Lee SS and Shin HK. 1994. Effect of dietary fiber source on the composition of intestinal microflora in rats. Korean J Nutr 27, 988-995
  21. Lim SN, Cheung PCK, Ooi VEC and Ang PO. 2002. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J Agric Food Chem 50, 3862-3866 https://doi.org/10.1021/jf020096b
  22. Lindsay GJH, Walton MJ, Adron JW, Fletcher TC, Cho CY and Cowey CB. 1984. The growth of rainbow trout (Salmo gairdneri) given diets containing chitin and its relationship to chitinolytic enzymes and chitin digestibility. Aquaculture 37, 315-334 https://doi.org/10.1016/0044-8486(84)90297-7
  23. Mayer AMS and Hamann MT. 2002. Marine pharmacology in 1999: compounds with antibacterial, anticoagulant, antifungal, anthelmintic, anti-inflammatory, antiplatelet, antiprotozoral and antiviral activities affecting the cardiovascular, endocrine, immune and nervous system, and other miscellaneous mechanisms of action. Comp Biochem Physiol Part C 132, 315-339 https://doi.org/10.1016/S1096-4959(02)00009-X
  24. Mayer AMS and Hamann MT. 2004. Marine pharmacology in 2000: Marine compounds with antibacterial, anticoagulant, antifungal, anthelmintic, anti-inflammatory, antiplatelet, antiprotozoral and antiviral activities affecting the cardiovascular, endocrine, immune and nervous system, and other miscellaneous mechanisms of action Mar Biotechnol 6, 37-52 https://doi.org/10.1007/s10126-003-0007-7
  25. Mohanty BR and Sahoo PK. 2007. Edwardsiellosis in fish: a brief review. J Biosci 32, 1331-1344 https://doi.org/10.1007/s12038-007-0143-8
  26. Okai Y, Okai KH, Ishizaka S and Yamashita U. 1997. Enhancing effect of polysaccharides from an edible brown alga, Hijikia fusiformes (Hijikia), on release of tumor necrosis factor alpha from macrophages of endotoxin-nonresponder C3H/HeJ mice. Nutr Cancer 27, 74-79 https://doi.org/10.1080/01635589709514505
  27. Okai Y, Okai KH, Ishizaka S, Ohtani K, Yuasa IM and Yamashita U. 1998. Possible immunomodulating activities in an extract of edible brown algae, Hijikia fusiformes(Hijikia). J Sci Food Agric 76, 56-62 https://doi.org/10.1002/(SICI)1097-0010(199801)76:1<56::AID-JSFA927>3.0.CO;2-L
  28. Park EY and Lee SS. 1996. Effect of dietary fiber on the serum lipid level and bowel function in aged rats. Korean J Nutr 29, 934-942 https://doi.org/10.3746/jkfn.2002.31.5.819
  29. Park JH, Kang KC, Baek SB, Lee YH and Rhee KS. 1991. Separation of antioxidant compounds from edible marine algae. Korean J Food Sci Technol 23, 256-261
  30. Park YB. 2005. Determination of nitrite-scavenging activity of seaweed. J. Korean Soc Food Sci Nutr 34, 1293-1296 https://doi.org/10.3746/jkfn.2005.34.8.1293
  31. Pham MA, Lee KJ, Lee BJ, Lim SJ, Kim SS, Lee YD, Heo MS and Lee KW. 2006. Effects of dietary Hizikia fusiformis on growth and immune responses in juvenile olive flounder (Paralichthys olivaceus). Asian-Aust. J Anim Sci 19, 1769-1775 https://doi.org/10.5713/ajas.2006.1769
  32. Ruperez P, Ahrazem O and Leal JA. 2002. Potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed Fucus vesiculosus. J Agric Food Chem 50, 840-845 https://doi.org/10.1021/jf010908o
  33. Sae-Dui D, Muroga K and Nakai T. 1984. A case of Edwardsiella tardain cultured colored carp, Cyprinius carpio. Fish Pathol 19, 197-199 https://doi.org/10.3147/jsfp.19.197
  34. Shan BE, Yoshida Y, Koroda E and Yamashita U. 1999. Immunomodulating activity of seaweed extract on human lymphocytes in vitro. Int. J Immunopharmaco 21, 59-70 https://doi.org/10.1016/S0192-0561(98)00063-0
  35. Shiau SY and Yu YP. 1999. Dietary supplementation of chitin and chitosan depresses growth in tilapia, Oreochromis niloticus ${\times}$ O aureus. Aquaculture 179, 439-436 https://doi.org/10.1016/S0044-8486(99)00177-5
  36. Shim YY, An JH, Cho WD, Chun H, Kim KI, Cho HY and Yang HC. 2002. Inhibitory mechanism of blood coagulation and in vivo anticoagulant activities of polysaccarides isolated from Codium fragile. J Korean Soc Food Sci Nutr 31, 917-923 https://doi.org/10.3746/jkfn.2002.31.5.917
  37. Skerget M, Kotnik P, Hadolin M, Hras AR, Simonic M and Knez Z. 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and the antioxidant activities. Food Chem 89, 191-198 https://doi.org/10.1016/j.foodchem.2004.02.025
  38. Yamamoto L, Nagumo T, Takahashi M, Fujihara M, Suzuki Y and Lizima N. 1981. Antimutagenic effect of seaweeds: Ⅲ. Antitumor effect of an extract from Sagassum. Jap J Exp Med 51, 187- 189
  39. Yoon JA, Yu KW, Jun WJ, Cho HY, Son YS and Yang HC. 2000. Screening of anticoagulant activity in the extracts of edible seaweeds and optimization of extraction condition. J Korean Soc Food Sci Nutr 29, 1098-1106

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