Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration from Mature Seed Culture of Miscanthus sinensis

억새 성숙종자로부터 캘러스 유도 및 식물체 재분화에 있어서 식물생장호르몬의 영향

  • Park, Choong-Hoon (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Yong-Goo (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Kyung-Hee (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Alam, Iftekhar (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Hyo-Jin (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Sharmin, Shamima Akhtar (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Ki-Won (National Institute of Animal Science, RDA) ;
  • Lee, Byung-Hyun (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 박충훈 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 김용구 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 김경희 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 알람 이프테칼 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 이효진 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 샤르민 샤미마 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 이기원 (농촌진흥청 국립축산과학원) ;
  • 이병현 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • Published : 2009.12.31
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Abstract

In order to optimize tissue culture conditions for genetic transformation of Miscanthus sinensis, we investigated the effects of different plant growth regulators on callus induction and plant regeneration using mature seeds as explant. Dehusked mature seeds were cultured on MS medium supplemented with 3 to 10 mg/L 2,4-D, dicamba or NAA, 30 g/L sucrose and 750 mg/L $MgCl_2{\cdot}6H_2O$. A number of combinations of auxin and cytokinin (BA, kinetin) were also used. MS medium containing 3 mg/L 2,4-D was found optimal for embryogenic callus induction (75.7%) from mature seed. The highest number of plants were regenerated (44.6%) upon transferring the embryogenic callus to MS medium supplemented with 1 mg/L 2,4-D plus 2 mg/L BA. This high efficient plant regeneration system could be useful to use for molecular breeding of new cultivars by genetic transformation.

억새의 최적 조직배양조건을 확립하기 위하여 성숙종자로부터 배발생 캘러스 유도 및 캘러스로부터 식물체 재분화에 미치는 식물생장 조절물질의 영향을 조사하였다. 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 3 mg/L 2,4-D가 첨가된 배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었다. 식물체 재분화는 배발생 캘러스를 1 mg/L 2,4-D와 2 mg/L BA가 첨가된 재분화 배지에서 배양했을 때 가장 높은 재분화효율을 보여주었다. 본 연구를 통하여 확립된 효율적인 배발생 캘러스의 유도 및 식물체 재분화 체계는 억새의 신품종 개발을 위한 분자육종 기술 확립에 유용하게 이용 될 수 있을 것이다.

Keywords

References

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