Parasites, Hosts and Diseases

  • 제47권1호
  • /
  • Pages.65-68
  • /
  • 2009
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

Ultrastructural Localization of Toxocara canis Larval Antigen Reacted with a Seropositive Human Serum

  • Lee, Soo-Ung (Department of Environmental and Tropical Medicine, School of Medicine, Konkuk University) ;
  • Yu, Jae-Ran (Department of Environmental and Tropical Medicine, School of Medicine, Konkuk University) ;
  • Huh, Sun (Department of Parasitology, College of Medicine, Hallym University)
  • 발행 : 2009.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Excretory-secretory products of Toxocara canis larvae have been considered as a major functional antigen in immune responses against toxocariasis. We studied ultrastructural localization of T. canis second-stage larval antigen using a seropositive human serum under immunogold electron microscopy. High-density gold particles were observed in the secretory cells, excretory duct, intestinal epithelium, and cuticle of the larval worm sections. The distribution of the positive reactions in the larval worms suggests that the nature of the antigen is excretory-secretory antigen including waste metabolites and secretory enzymes.

키워드

참고문헌

  1. Kim YH, Huh S, Chung YB. Seroprevalence of toxocariasis among healthy people with eosinophilia. Korean J Parasitol 2008; 46:29-32 https://doi.org/10.3347/kjp.2008.46.1.29
  2. Kim YH, Huh S. Prevalence of Toxocara canis, Toxascaris leonina and Dirofilaria immitis in dogs in Chuncheon, Korea (2004). Korean J Parasitol 2005; 43: 65-67 https://doi.org/10.3347/kjp.2005.43.2.65
  3. Choi D, Lim JH, Choi DC, Paik SW, Kim SH, Huh S. Toxocariasis and ingestion of raw cow liver in patients with eosinophilia. Korean J Parasitol 2008; 46: 139-143 https://doi.org/10.3347/kjp.2008.46.3.139
  4. Magnaval JF, Glickman LT, Dorchies P, Morassin B. Highlights of human toxocariasis. Korean J Parasitol 2001; 39: 1-11 https://doi.org/10.3347/kjp.2001.39.1.1
  5. Kondo K, Akao N, Konishi Y, Yoshimura H, Hirose H. Immunoelectron microscopic observation of excretory cell of Toxocara canis larva. Jpn J Parasitol 1987; 36: 187-189
  6. Yamasaki H, Araki K, Lim PKC, Zasmy N, Mak JW, Taib R, and Aoki T. Development of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory antigen for immunodiagnosis of human toxocariasis. J Clin Microbiol 2000; 38:1409-1413
  7. Page AP, Hamilton AJ, Maizels RM. Toxocara canis: monoclonal antibodies to carbohydrate epitopes of secreted (TES) antigens localize to different secretion-related structures in infective larvae. Exp Parasitol 1992; 75: 56-71 https://doi.org/10.1016/0014-4894(92)90122-Q
  8. Page AP, Rudin W, Fluri E, Blaxter ML, Maizels RM. Toxocara canis:a labile antigenic surface coat overlying the epicuticle of infective larvae. Exp Parasitol 1992; 75: 72-86 https://doi.org/10.1016/0014-4894(92)90123-R
  9. Bird AF. The Structure of Nematodes. London and New York. Academic Press. 1971, p 178-197
  10. Nelson FK, Albert PS, Riddle DL. Fine structure of the Caenorhabditis elegans secretory-excretory system. J Ultrastruc Res 1983;82: 156-171 https://doi.org/10.1016/S0022-5320(83)90050-3
  11. Park SP, Park I, Park HY, Lee SU, Huh S, Magnaval JF. Five cases of ocular toxocariasis confirmed by serology. Korean J Parasitol 2000; 38: 267-273 https://doi.org/10.3347/kjp.2000.38.4.267
  12. de Savigny DH. In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. J Parasitol 1975; 61: 781-782 https://doi.org/10.2307/3279492
  13. Glickman LT, Magnaval JF, Domanski LM, Shofer FS, Lauria SS, Gottstein B, Brochier B. Visceral larva migrans in French adults:a new disease syndrome? Am J Epidemiol 1987; 125: 1019-1034
  14. Magnaval JF, Galindo V, Glickman LT, Clanet M. Human Toxocara infection of the central nervous system and neurological disorders:a case-control study. Parasitology 1997; 115: 537-543 https://doi.org/10.1017/S0031182097001558
  15. McGillivery DJ, Yong WK, Riffkin GG, Adler B. The distribution and localization of the stage-specific GP31 antigen from infective Ostertagia circumcincta larvae. Int J Parasitol 1990; 20: 87-93 https://doi.org/10.1016/0020-7519(90)90178-P
  16. Subrahmanyam D, Parab PB, Rajasekariah GR, Rudin W, Betschart B, Weiss N. Interaction of monoclonal antibodies with cuticular antigens of filarial parasites, Brugia malayi and Wuchereria bancrofti. Acta Trop 1990; 47: 381-390 https://doi.org/10.1016/0001-706X(90)90039-3

피인용 문헌

  1. Producción y evaluación del antígeno recombinante Tes-30 de Toxocara canis para el inmunodiagnóstico de toxocariasis vol.36, pp.1, 2009, https://doi.org/10.7705/biomedica.v36i1.2617