동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제23권1호
  • /
  • Pages.84-92
  • /
  • 2009
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

반하추출물(半夏抽出物)이 자발적 원형탈모 생쥐와 정상 생쥐의 발모효과에 대한 실험적 연구

Experimental Studies on the Hair Growth Activity of Extracts of Pinelliae Rhizoma in Spontaneous Alopecia Model and Normal C57BL/6N Mice

  • 정일국 (우석대학교 한의과대학 해부학교실) ;
  • 조한영 (우석대학교 한의과대학 해부학교실) ;
  • 김태헌 (우석대학교 한의과대학 해부학교실) ;
  • 김남석 (우석대학교 한의과대학 해부학교실) ;
  • 정한솔 (우석대학교 한의과대학 병리학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실)
  • Jeong, Il-Kook (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Jo, Han-Young (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Kim, Tae-Heon (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Kim, Nam-Seok (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
  • 발행 : 2009.02.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This experiment was investigated the effects of fresh and ginger processed Pinelliae Rhizoma extracts on hair growth activity, and its fractions(chloroform, ethyl acetate and water fractions) obtained from fresh Pinelliae Rhizoma on hair growth activity of the normal and spontaneous alopecia areata model of C57BL/6N mice for 16 days. The results were as follows: In fresh Pinelliae Rhizoma extracts treated group, hair growth effect was observed in whole skin area(100%) all the normal mice in whose hair had been clipped on 16th days. In ginger processed Pinelliae Rhizoma extracts treated group, hair growth effect was observed in whole skin area in 25% of normal mice in whose hair had been clipped on 16th days. But in control group, hair growth effect was observed in a part of whole skin area in 25% of normal mice. In fresh Pinelliae Rhizoma extracts treated group, hair follicles of middle stage of anagen phase was observed and it were grown down to subcutaneous tissue of skin in all the mice on 10th day. But in ginger processed Pinelliae Rhizoma extracts treated group and control group, Most of hair follicles of telogen phase was observed in skin. The treatment of extracts of fresh Pinelliae Rhizoma increased the expression of TGF-$\beta$(146%), IGF(107%), and prolactin(115%) in the skin of normal C57BL/6N mice compared to control group(100%). But expression of placenta lactogen(93%) was decreased in the skin of normal C57BL/6N mice compared to control group(100%). In spontaneous alopecia model, The hair growth activity of fresh Pinelliae Rhizoma extracts treated group(100%) was observed to be strong compared with the control group(20%) on 15th day. Hair growth activity on chloroform fractions of fresh Pinelliae Rhizoma extracts was observed in whole skin area in 75% of normal mice on the 9th day. In water and ethyl acetate fractions, hair growth activity was observed in a part of whole skin in 75% and 25% of normal mice, respectively. but hair growth activity of control group was not observed. After application of fractions of fresh Pinelliae Rhizoma extracts for 10 days, hair follicles of chloroform fraction treated group was observed middle stage of anagen phase and hair follicle were grown down to subcutaneous tissue of skin in all the mice. But hair follicles of initial stage of anagen phase were observed in water and ethyl acetate fractions. Most of hair follicles of telogen phase was observed in skin of control group. These experiments suggest that extracts of fresh Pinelliae Rhizoma may stimulate the topical hair growth activity and its chloroform fractions can be useful for treatment of alopecia areata.

키워드

참고문헌

  1. Headington, J.T. Histological findings in androgenic alopecia treated with topical minoxidil. Br J Dermatol 107(Suppl. 22):20-21, 1982
  2. 문정배, 김영진, 이태후. 한의학적 탈모 치료효과의 객관적 평가 방법. 대한한의학회지 27(2):57-69, 2006
  3. 이태후, 문정배, 안경애, 이혜정. 탈모 환자의 적외선 체열 진단상 안면부 체열 특성. 대한한의학회지 27(2):249-252, 2006
  4. 윤신혁. 다양한 發毛成長 物質에 대한 動物 實驗 및 分子生物學籍 接近:아젤라인산 및 비타민 B6의 효과. 대구가톨릭대학교 대학원, 석사학위논문, 2007
  5. 최근희 외 11인, 모발과학, 수문사, pp 138-140, 2001
  6. 馬紹堯, 趙尙華. 現代中醫皮膚性病診療大全, 山西科學技術出版社, p 957, 1998
  7. Selmanowilz, V., Victor, S., Warburton, D., Orentreich, N. Fingerprints arches in alopecia areata. Arch Dermatol 110: 570-571, 1974 https://doi.org/10.1001/archderm.110.4.570
  8. Muller, H.K., Rook, A.J., Kubba, R. Immunohistology and autoantibody studies in alopecia areata. Br J Dermatol 102: 609-610, 1980 https://doi.org/10.1111/j.1365-2133.1980.tb07664.x
  9. Friedman, P.S. Alopecia areata and auto-immunity. Br J Dermatol 105: 153-157, 1981 https://doi.org/10.1111/j.1365-2133.1981.tb01200.x
  10. 黃霏莉. 余靖. 中醫美容學, 人民衛生出版社, pp 192-193, 1997
  11. 周鳴岐. 脫髮證治. 中醫雜誌 27(12):47, 1986
  12. Civatte, J. Topical 2% minoxidil solution in mail pattern alopecia. The initial European Experience. Int J Dermatol 27: 424, 1988 https://doi.org/10.1111/j.1365-4362.1988.tb00002.x
  13. Schuttelaar, M.L., Hamstra, J.J., Plinck, E.P., Peereboom- Wynia, J.D., Vuzevski, V.D., Mulder, P.G., Oranje, A.P. Alopecia areata in children : treatment with diphencyprone. Br J Dermatology 135(4):581-585, 1996 https://doi.org/10.1111/j.1365-2133.1996.tb03835.x
  14. 윤정훈, 기경헌, 장수진, 신민섭. 약침을 이용한 원형탈모증 치험 1예. 대한외관과학회지 14(1):1005-1010, 2001
  15. 김경운, 서정철, 이경민, 임성철, 정태영, 한상원. 봉약침과 홍화약침을 이용한 원형탈모증 치험 1예. 대한약침학회지 7(2):91-96, 2004 https://doi.org/10.3831/KPI.2004.7.2.091
  16. 김소영, 김중호, 채병윤. 탈모에 응용되는 신응양진단의 효능에 관한 실험적 연구. 대한외관과학회지 4(1):43-58, 1991
  17. 권강주, 이창현, 이문원, 문연자, 최두호, 이학재, 김호민, 우원홍. 사물탕가미방이 원형탈모 생쥐의 발모효과에 관한 형태학적 연구. 동의생리병리학회지 19(3):696-704, 2005
  18. 이희삼, 문재유. Cytarabine으로 유도된 탈모증에 대한 상백피 추출물의 육모촉진 효과. 약학회지 44(1):66-70, 2000
  19. 노현찬, 노석선. 고삼추출물이 모발성장 촉진 및 면포억제에 미치는 영향. 대한안이비인후피부과학회지 15(1):96-126, 2002
  20. 전국한의과대학 본초학교수편. 본초학. 서울, 영림사, pp. 448-449, 1994
  21. 박지하, 서부일. 반하의 독성에 관한 문헌적 고찰. 동서의학 25(3):47-55, 2000
  22. 강선태, 이태호. 어혈병태모형에 미치는 천궁, 석창포 및 반하의 효능에 관한 실험적 연구. 동의병리학회지 4(1):57-73, 1989
  23. 송재진. 반하가 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대전대학교 대학원, 박사학위논문, 2005
  24. Birch, D.E., Kolmodin, L., Laird, W.J., McKinney, N., Wong, J., Young, K.K.Y., Zangenberg, G.A., Zoccoli, M.A. Simplified Hot-Start PCR. Nature 381: 445-446, 1996 https://doi.org/10.1038/381445a0
  25. 장해영, 최관호, 김성현 외. 탈모에 대한 문헌적 고찰. 대한약침학회지 5(2):92-108, 2002 https://doi.org/10.3831/KPI.2002.5.2.092
  26. 越 佶. 聖濟總錄. 北京, 人民衛生出版社, pp 1778-1779. 1987
  27. 裵元植. 東西醫學比較 最新漢方臨床學. 서울, 남산당, pp 659-661, 1991
  28. 許 浚. 동의보감. 서울, 법인문화사, pp 793-797, 1999
  29. 巢元方. 諸病源候論校釋. 서울, 대성출판사, pp 197-200, 1992
  30. 葛正義. 血液流變學輿甲皺微循環檢測對脂漏性脫髮中醫分型及治療的臨床意義. 淅江中西醫結合雜紙 21: 107, 1998
  31. 徐建榮, 朱拉珍, 陳凌風. 步長腦心通聯合銀杏葉片治療斑禿前後甲襞微循環觀察. 河北中醫, 22(1):42, 2000
  32. 許 浚. 동의보감. 서울, 법인문화사, p 800, 1999
  33. 杜錫賢. 皮膚病中醫外治療法. 北京, 中國醫藥科技出版社, pp 11, 2000
  34. 王見宾, 張毅. 斑禿外用中藥劑型. 中國醫學文摘 皮膚科學, 20(5):338-340, 2003
  35. Argyris, T.S. The effects of wounds on adjacent growing or resting hair follicles in mice. Arch Dermatol Symp 61: 31-36, 1956
  36. Argyris, T.S. Kinetics of epidermal production during epidermal regeneration following abrasion in mice. Am J Pathol 83: 329-340, 1956
  37. Lynfield, Y.L., Macwilliams, P. Shaving and hair growth. J Invest Dermatol 55: 170-172, 1970 https://doi.org/10.1111/1523-1747.ep12280667
  38. MacDonald, H.S., Cunliffe, W.J. Alopecia areata treated with diphencyprone sensitization. Clin Exp Deramtol 14: 154-157, 1989 https://doi.org/10.1111/j.1365-2230.1989.tb00915.x
  39. Silver, A.F., Chase, H.B., Arsenault, C.T. Early anagen initiated by plucking compared with early spontaneous anagen. Adv Biol Skin 9: 265-286, 1969
  40. Stenn, K.S., Paus, R. Controls of hair follicle. Physiological Reviews 81: 449-494, 2001 https://doi.org/10.1152/physrev.2001.81.1.449
  41. 類國菁. 發泡療法及其臨床應用. 時珍國醫國藥, 12(12):1126-1128, 2001
  42. Slominski, A., Wortsman, J. Neuroendocrinology of the skin. Endocr Rev 21: 457-487, 2000 https://doi.org/10.1210/er.21.5.457
  43. Daughaday, W.H., Rotwein, P. Insulin-like growth factors I and II. Peptide, messenger ribonucleic acid and gene strucures, serum, and tissue concentrations. Endocr Rev 10: 68-91, 1989 https://doi.org/10.1210/edrv-10-1-68
  44. Su, H.Y., Hickford, J.G., Bickerstaffe, R., Palmer, B.R. Insulin-like growth factor 1 and hair growth. Dermatol Online J 5: 1, 1999
  45. Itami, S., Inui, S. Role of androgen in mesenchymal epithelial interactions in human hair follicle. J Invest Dermatol Symp Proc 10: 209-211, 2005 https://doi.org/10.1111/j.1087-0024.2005.10107.x
  46. Tavakkol, A., Elder, J.T., Griffiths, C.E., Cooper, K.D., Talwar, H., Fisher, G.J., Keane, K.M., Foltin, S.K., Voorhees, J.J. Expression of growth hormone receptor, insulin-like growth factor 1(IGF-1) and IGF-1 receptor mRNA and proteins in human skin. J Invest Dermatol 99: 343-349, 1992 https://doi.org/10.1111/1523-1747.ep12616668
  47. Jiang, H., Yamamoto, S., Kato, R. Induction of anagen in telogen mouse skin by topical application of FK506, a potent immunosuppressant. J Invest Dermatol 104: 523-525, 1995 https://doi.org/10.1111/1523-1747.ep12606018
  48. Pearson, A.J., Ashby, M.G., Wildermoth, J.E., Craven, A.J., Nixon, A.J. Effect of exogenous prolactin on the hair growth cycle. Exp Dermatol 8: 358-360, 1999
  49. Harada, N., Okajima, K., Arai, M., Kurihara, H., Nakagata, N. Administration of capsaicin and isoflavone promotes hair growth by increasing insulin-like growth factor-I production in mice and in humans with alopecia. Growth Hormone & IGF Research 17: 408-415, 2007 https://doi.org/10.1016/j.ghir.2007.04.009
  50. Vignery, A., McCarthy, T.L. The neuropeptide calcitonin gene-related peptide stimulates insulin-like growth factor-I production by primary fetal rat osteoblasts. Bone 18: 331-335, 1996 https://doi.org/10.1016/8756-3282(96)00017-8
  51. Foitzik, K., Krause, K., Nixon, A.J., Ford, C.A., Ohnemus, U., Pearson, A.J., Paus, R. Prolactin and its receptor are expressed in murine hair follicle epithelium, show hair cycle-dependent expression, and induce catagen. Am J Pathol 162: 1611-1621, 2003 https://doi.org/10.1016/S0002-9440(10)64295-2