Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Leukotriene B4 Regulates Proliferation and Differentiation of Cultured Rat Myoblasts via the BLT1 Pathway

  • Sun, Ru (Institute of Genetics and Cytology, Northeast Normal University) ;
  • Ba, Xueqing (Institute of Genetics and Cytology, Northeast Normal University) ;
  • Cui, Lingling (Institute of Genetics and Cytology, Northeast Normal University) ;
  • Xue, Yan (Institute of Genetics and Cytology, Northeast Normal University) ;
  • Zeng, Xianlu (Institute of Genetics and Cytology, Northeast Normal University)
  • Received : 2008.09.29
  • Accepted : 2009.01.19
  • Published : 2009.04.30
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Abstract

Skeletal muscle regeneration is a highly orchestrated process initiated by activation of adult muscle satellite cells. Upon muscle injury, the inflammatory process is always accompanied by muscle regeneration. Leukotriene $B_4$ is one of the essential inflammatory mediators. We isolated and cultured primary satellite cells. RT-PCR showed that myoblasts expressed mRNA for $LTB_4$ receptors BLT1 and BLT2, and $LTB_4$ promoted myoblast proliferation and fusion. Quantitative real-time PCR and immunoblotting showed that $LTB_4$ treatment expedited the expression process of differentiation markers MyoD and M-cadherin. U-75302, a specific BLT1 inhibitor, but not LY2552833, a specific BLT2 inhibitor, blocked proliferation and differentiation of myoblasts induced by $LTB_4$, which implies the involvement of the BLT1 pathway. Overall, the data suggest that $LTB_4$ contributes to muscle regeneration by accelerating proliferation and differentiation of satellite cells.

Keywords

Acknowledgement

Supported by : National Natural Science Foundation of China, NENU

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