Journal of Korean Society of Water and Wastewater (상하수도학회지)

The Korean Society of Water and Wastewater (대한상하수도학회)
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Toluene Removal and Microbial Growth of Candida tropicalis Immobilized with Polymer Media in Airlift Bioreactors

효모 Candida tropicalis 고정화 담체를 이용한 Airlift 미생물반응기의 톨루엔 제거 및 미생물 성장

  • 남궁형규 (세종대학교 토목환경공학과) ;
  • 송지현 (세종대학교 토목환경공학과) ;
  • 정미영 (경희대학교 환경응용화학대학 환경공학전공) ;
  • 황선진 (경희대학교 환경응용화학대학 환경공학전공)
  • Received : 2008.12.29
  • Accepted : 2009.01.22
  • Published : 2009.04.15
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Abstract

This study was conducted to improve biological degradation efficiency of toluene as a model volatile organic compound (VOC) using yeast Candida tropicalis and to suggest an effective method for bioreactor operation. The yeast strain was immobilized with polyethylene glycol (PEG), alginate, and powdered activated carbon (PAC). The yeast-immobilized polymer media were used as fluidized materials in an airlift bioreactor. Polymer media without PAC were also made and operated in another airlift bioreactor. The two bioreactors showed toluene removal efficiencies ranging 80-96% at loading rates of $10-35 g/m^3-hr$, and the bioreactor containing the polymer media with PAC achieved higher removal efficiency. Protein contents in the liquid phase showed that the bioreactor using the yeast-immobilized polymer media with PAC had a higher rate of microbial growth initially than that without PAC. In addition, the microbial growth rate inside of the polymer media with PAC was five times higher than that without PAC. Consequently, the polymer media containing the yeast strain and PAC could enhance removal efficiencies for VOCs, and the immobilization method improve microbial activity and stability for a long-term operation of biological systems.

Keywords

Acknowledgement

Supported by : 환경부

References

  1. 김성빈, 김치경, 김희식, 이창호, 신기선, 권기석, 윤병대, 오희목(1996) 페놀분해 효모 Candida tropicalis PW-51의 분리 및 분해특성, 산업미생물학회지, 제24권, 제6호, pp.743-748
  2. 김조천 (2006) 국내의 휘발성유기화합물(VOC) 현황 및 관리기술, 한국대기환경학회지, 제22권, 제6호, pp.743-757
  3. 남궁완, 박용진, 박준석 (2000) GAC 및 퇴비를 바이오필터를 이용한 고농도 톨루엔가스의 처리, 한국폐기물학회지, 제17권, 제8호, pp.969-967
  4. 류희욱, 장용근, 김상돈(1994) Airlift 생물 반응기, 한국생물공학회지, 제9권, 제4호, pp.347-364
  5. 조영철, 장현섭, 황선진(2007) 미생물반응기에서 분리한 Pseudomonas 속 세균의 BTXS Compounds 분해 특성, 대한환경공학회지, 제29권, 제6호, pp.678-683
  6. 하태욱, 최상준(2000) 악취 및 VOCs 제어를 위한 Biofilter 기술, 대한환경공학회지, 제14권, pp.14-23
  7. Lo, C.S and Hwang, S.J (2004) Dynamic behavior of an internal-loop airlift bioreactor for degradation of waste gas containing toluene, Chemical Engineering Science, Vol 59, pp.4517-4530 https://doi.org/10.1016/j.ces.2004.07.002
  8. Devinny, J.S., Deshusses, M.A. and Webster T.S. (1999) Biofiltration for air pollution control, CRC Lewis Publishers, New York
  9. Van Groenestijn, J.W., Van Heiningen, W.N.M. and Kraakman, N.J.R. (2001) Biofilters based on the action of fungi, Water Sci. Technol., Vol 44(9), pp.227–232
  10. Jung, I.G. and Park, C.H. (2004) Characteristics of Rhodococcus pyridinovorans PYJ-1 for the biodegradation of benzene, toluene, m-xylene (BTX), and their mixtures, J. Biosci. Bioeng., Vol 97(6), pp.429-431
  11. Kennes, C. and Veiga, M.C. (2001) Bioreactors for waste gas treatment, Kluwer Academic Publishers, Norwell
  12. Kim. T.Y., Jin. H.J., Park. S.S., Kim S.J. and Cho. S.Y. (2008) Adsorption equilibrium of copper and phenol by powdered activated carbon , alginate bead and alginate-activated carbon bead, Journal of Industrial and Engineering Chemistry, Vol 14, pp.714-719 https://doi.org/10.1016/j.jiec.2008.07.004
  13. Kinney, K.A., Loehr, R.C. and Corsi, R.L. (1999) Vapor-phase bioreactors: avoiding problems through better design and operation, Environ. Prog., Vol 18, pp.222-230 https://doi.org/10.1002/ep.670180319
  14. Neal, A.B. and Loehr, R.C. (2000) Use of biofilters and suspended-growth reactors to treat VOCs, Waste Manage., Vol 20, pp.59-68 https://doi.org/10.1016/S0956-053X(99)00297-4
  15. Oh. K.J., Cho. K.C., Choung. Y.H., Park. S.K., Cho. S.K. and Kim. D.G. (2006) Removal of hydrogen sulfide, benzene and toluene by a fluidized bed bioreactor, Korean J. Chem. Eng., Vol 23(1), pp.148-152 https://doi.org/10.1007/BF02705707
  16. Shim, H. and Yang, S.T. (1999) Biodegradation of benzene, toluene, ethylbenzene, and o-xylene by a coculture of Pseudomonas putida and Pseudomonas fluorescens immobilized in a fibrous-bed bioreactor, J. Biotechnol., Vol 67(2-3), pp.99-112 https://doi.org/10.1016/S0168-1656(98)00166-7
  17. Stefanie. D., Aude. G., Cecile. L. and Aissa, O,D. (2008) Solid-liquid mass transfers in an Airlift Reactor incorporating alginate beads for the application as bioartificial liver, Chemical Engineering and Process., Vol 47(12), pp.2370-2378 https://doi.org/10.1016/j.cep.2008.01.013
  18. Xiaoping. L., Jian. D., Yanru. W., and Jun. S. (2000) Comparison of the hydrodynamics and mass transfer characteristics of a modified square airlift reactor with common airlift reactors, Chemical Engineering Science, Vol 55, pp.2257-2263 https://doi.org/10.1016/S0009-2509(99)00473-X
  19. Yan, J., Jianping, W., Hongmei, L., Suliang, Y. and Zongding, H. (2005) The biodegradation of phenol at high initial concentration by the yeast Candida tropicalis, Biochem. Eng. J., Vol 24(3), pp.243-247 https://doi.org/10.1016/j.bej.2005.02.016
  20. Yan, J., Jianping, W., Jing, B., Daoquan, W. and Zongding, H. (2006) Phenol biodegradation by the yeast Candida tropicalis in the presence of m-cresol, Biochem. Eng. J., Vol 29(3), pp.227-234 https://doi.org/10.1016/j.bej.2005.12.002