멸종위기에 처한 얼룩새코미꾸리 Koreocobitis naktongensis (Cobitidae)의 난 발생 및 자치어 형태 발달

Morphological Development of Egg and Larvae of Koreocobitis naktongensis (Cobitidae)

  • 송하윤 (순천향대학교 해양생명공학과) ;
  • 양현 (생물다양성연구소) ;
  • 조은미 (순천향대학교 생물학과) ;
  • 신현철 (순천향대학교 생물학과) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Song, Ha-Yoon (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Yang, Hyun (Institute of Biological Diversity Research) ;
  • Cho, Eun-Mi (Department of Biology, Soonchunhyang University) ;
  • Shin, Hyunchur (Department of Biology, Soonchunhyang University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 투고 : 2009.11.23
  • 심사 : 2009.12.26
  • 발행 : 2009.12.31

초록

멸종위기 담수어류인 얼룩새코미꾸리 Koreocobitis naktongensis의 난 발생 과정 및 자치어 형태 발달을 조사하였다. 인간 융모성 성선자극호르몬(HCG)을 10 IU/g의 농도로 암컷의 복강에 주사하여 성숙란을 얻었으며, 건식법으로 정자와 수정시켰다. 수정란의 난경은 1.0~1.2 mm로 유구는 존재하지 않았다. 배체는 수온 $20{\pm}1^{\circ}C$에서 수정 38시간 후부터 부화하기 시작하였다. 부화 직후 자어의 전장은 2.5~2.8 mm로 입과 항문은 아직 열리지 않았다. 부화 후 7일째 자어는 전장 4.5~5.2 mm로 난황은 완전히 흡수되었다. 부화 후 50일째 모든 지느러미가 완성되는 치어기로 이행하였고, 이때의 전장은 21~27 mm였다.

The egg development and early life history of the Korean endangered fish Koreocobitis naktongensis (Cobitidae) were investigated. Eggs from the females were obtained by injecting 10 IU/g of human chorionic gonadotropin and inseminated with sperm by the dry method in the laboratory. The fertilized eggs were 1.0~1.2 mm in diameter and had no oil globules. The embryo began to hatch about 38 hrs after fertilization under water temperature of $20{\pm}1^{\circ}C$. The newly-hatched larvae were 2.6~2.8 mm in total length, and their mouth and anus were not yet opened. Seven days after hatching, the postlarvae were 4.5~5.2 mm in total length, and their yolk sacs were completely absorbed. They entered the juvenile stage when all fin-rays were formed at 50 days after hatching, and their total lengths were 21~27 mm.

키워드

과제정보

연구 과제 주관 기관 : 환경부

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