Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Non-ionic Surfactant Supplementation on Ruminal Fermentation, Nutrient Digestibility and Performance of Beef Steers Fed High-roughage Diets

  • Ahn, Gyu-chul (Animal Resources Research Center, College of Animal Bioscience and Technology Konkuk University) ;
  • Kim, Jeong-hoon (Animal Welfare Program, Faculty of Land and Food Systems, University of British Columbia) ;
  • Park, Eun-kyu (Animal Resources Research Center, College of Animal Bioscience and Technology Konkuk University) ;
  • Oh, Young-Kyoon (National Institute of Animal Science) ;
  • Lee, Gang-yeon (Animal Resources Research Center, College of Animal Bioscience and Technology Konkuk University) ;
  • Lee, Jung-il (Animal Resources Research Center, College of Animal Bioscience and Technology Konkuk University) ;
  • Kim, Chong-min (Animal Resources Research Center, College of Animal Bioscience and Technology Konkuk University) ;
  • Park, Keun-kyu (Animal Resources Research Center, College of Animal Bioscience and Technology Konkuk University)
  • Received : 2009.02.03
  • Accepted : 2009.04.10
  • Published : 2009.07.01
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Abstract

Three experiments were conducted to determine the effects of non-ionic surfactant (NIS) supplementation on ruminal fermentation, nutrient digestibility and performance of beef steers fed high-roughage diets. The objective of experiment 1 was to investigate the effects of NIS supplementation on in vitro ruminal fermentation of cultures administered with corn and barley as grain substrate and rice straw and timothy hay as roughage substrate. The in vivo ruminal fermentation, nitrogen balance and digestibility of nutrients were also examined with steers fed a high-roughage diet in experiment 2. The aim of experiment 3 was to determine the responses to NIS of growing steers fed a high-roughage diet. In experiment 1, ammonia nitrogen concentration for NIS supplementation was higher (p<0.05) than for the control with all substrates. However, concentrations of total volatile fatty acid (VFA), acetate, butyrate and valerate of the incubated roughage substrates, rice straw and timothy hay, were higher (p<0.05) for NIS supplementation than for the control whereas VFA concentrations in the cultures of corn and barley were unaffected. These results indicated that effects of NIS on ruminal fermentation are diet dependent, specifically on roughage sources. In experiment 2, ruminal pH of steers supplemented with NIS was lower (p<0.05) than the control. Ruminal concentrations of ammonia nitrogen, acetate, total VFA and urinary concentrations of purine derivatives were increased (p<0.05) by NIS supplementation. In experiment 3, supplementation of NIS increased (p<0.05) intakes of total feed and corn silage, average daily gain, and feed efficiency of growing steers although they varied depending on supplementation level. Due to the roughage-specific feature of NIS effects, NIS appears to enhance ruminal fermentation of fibrous parts of feeds and, consequently, performance of steers fed a high-roughage diet.

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References

  1. Akin, D. E. 1980. Evaluation by electron microscopy and anaerobic culture of types of rumen bacteria associated with digestion of forage cell wall. Appl. Environ. Microbiol. 39:242-252
  2. AOAC. 1990. Official methods of analysis, 15th Edition. Association of Official Analytical Chemists, Arlington, Virginia, USA
  3. Borchers, R. 1977. Allantoin determination. Anal. Biochem. 79:612-613 https://doi.org/10.1016/0003-2697(77)90443-2
  4. Castanon, M. and C. T. Wilke. 1981. Effects of the surfactant Tween 80 on enzymatic hydrolysis of newspaper. Biotechnol. Bioeng. 23:1365-1372 https://doi.org/10.1002/bit.260230615
  5. Chaney, A. L. and E. P. Markbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Biochem. 8:130-137
  6. Duncan, D. B. 1955. Multiple range and multiple F test. Biometrics 11:1-4 https://doi.org/10.2307/3001478
  7. Erwin, E. S., G. T. Marco and E. M. Emery. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44:1768-1771
  8. Farrance, I. 1987. Plasma glucose methods, a review. Clin. Biochem. Rev. 8:55-68
  9. Goering, H. K. and P. J. Van Soest. 1970. Forage fiber analysis (apparatus, reagent, procedures and some applications). Agric. Handbook No. 379. ARS-USDA. Washington. DC. USA
  10. Goto, M., H. D. Bae, M. S. Yahaya, S. Karita, K. Wanjae, K. Sugawara and K. J. Cheng. 2003a. Effects of surfactants Tween 80 on enzymatic accessibility and degradation of orchardgrass Tween 80 on enzymatic accessibility and degradation of orchardgrass (Dactylis gromerata L.) at different growth stages. Asian-Aust. J. Anim. Sci. 16:83-87
  11. Goto, M., H. D. Bae, S. S. Lee, M. S. Yahaya, S. Karita, K. Wanjae and K. J. Cheng. 2003b. Effects of surfactant Tween 80 on forage degradability and microbial growth on the in vitro rumen mixed and pure cultures. Asian-Aust. J. Anim. Sci. 16:672-676
  12. Helle, S. S., S. J. B. Duff and D. G. Cooper. 1993. Effect of surfactants on cellulose hydrolysis. Biotechnol. Bioeng. 42:611-617 https://doi.org/10.1002/bit.260420509
  13. Hiltner, P. and B. A. Dehority. 1983. Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria. Appl. Environ. Microbiol. 46:642-648 https://doi.org/10.1016/0377-8401(86)90106-9
  14. Hristov, A. N., T. A. McAllister, M. E. Olson, K. J. Cheng, L. J. Yanke and J. A. Shelford. 2000. Effect of Tween 80 and salinomycin on ruminal fermentation and nutrient digestion in steers fed a diet containing 70% barley. Can. J. Anim. Sci. 80:363-372 https://doi.org/10.4141/A99-067
  15. Kaar, W. E. and M. Holtzapple. 1998. Benefits from Tween during enzymic hydrolysis of corn stover. Biotechnol. Bioeng. 59:419-427 https://doi.org/10.1002/(SICI)1097-0290(19980820)59:4<419::AID-BIT4>3.0.CO;2-J
  16. Kamande, G. M., J. Baah, K. J. Cheng, T. A. McAllister and J. A. Shelford. 2000. Effects of Tween 60 and Tween 80 on protease activity, thiol group reactivity, protein absorption and cellulose degradation by rumen microbial enzymes. J. Dairy Sci. 83:536-542 https://doi.org/10.3168/jds.S0022-0302(00)74913-7
  17. Kang-Meznarich, J. H. and G. A. Broderick. 1980. Effects of incremental urea supplementation on ruminal ammonia concentration and bacterial protein formation. J. Anim. Sci. 51:422-431
  18. Kennedy, P. M. and L. P. Milligan. 1980. The degradation and utilization of endogenous urea in the gastrointestinal tract of ruminants: a review. Can. J. Anim. Sci. 60:205-221 https://doi.org/10.4141/cjas80-030
  19. Kim, C. H., J. N. Kim, J. K. Ha, S. G. Yun and S. S. Lee. 2004. Effects of dietary addition of surfactant Tween 80 on ruminal fermentation and nutrient digestibility of Hanwoo steers. Asian-Aust. J. Anim. Sci. 17:337-342
  20. Kim, M. H., S. B. Lee and D. Y. Ryu. 1982. Surface deactivation of cellulase and its prevention. Enzyme Microb. Technol. 4:99-103 https://doi.org/10.1016/0141-0229(82)90090-4
  21. Kim, W. J., Y. Gamo, Y. M. Sani, Y. Wusiman, S. Ogawa, S. Karita and M. Goto. 2006. Effect of Tween 80 on hydrolytic activity and substrate accessibility of carbohydrolase I (CBH I) from Trichoderma viride. Asian-Aust. J. Anim. Sci. 19:684-689
  22. Kramer, C. Y. 1956. Extension of multiple range tests to group means with unequal numbers of replications. Biometrics 12:307-310 https://doi.org/10.2307/3001469
  23. Lee, C. H., H. G. Sung, M. Eslami, S. Y. Lee, J. Y. Song, S. S. Lee and J. K. Ha. 2007. Effects of Tween 80 pretreatment on dry matter disappearance of rice straw and cellulolytic bacterial adhesion. Asian-Aust. J. Anim. Sci. 20:1397-1401
  24. Lee, S. S. and J. K. Ha. 2003. Influences of surfactant Tween 80 on the gas production, cellulose digestion and enzyme activities by mixed rumen microorganisms. Asian-Aust. J. Anim. Sci. 16:1151-1157
  25. Lee, S. S., B. H. Ahn, H. S. Kim, C. H. Kim, K. J. Cheng and J. K. Ha. 2003. Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performances of lactating cows. Asian-Aust. J. Anim. Sci. 16:104-115
  26. Lee, S. S., H. S. Kim, Y. H. Moon, N. J. Choi and J. K. Ha. 2004. The effects of non-ionic surfactant on the fermentation characteristics, microbial growth, enzyme activity and digestibility in the rumen of cows. Anim. Feed Sci. Technol. 115:37-50 https://doi.org/10.1016/j.anifeedsci.2004.03.001
  27. Littell, R. C., P. R. Henry and C. B. Ammerman. 1998. Statistical analysis of repeated measures data using SAS procedures. J. Anim. Sci. 76:1216-1231
  28. McAllister, T. A., K. Stanford, H. D. Bae, R. J. Treacher, A. N. Hristov, J. Baah, J. A. Shelford and K. J. Cheng. 2000. Effect of a surfactant and exogenous enzymes on digestibility of feed and on growth performance and carcass traits of lambs. Can. J. Anim. Sci. 80:35-44 https://doi.org/10.4141/A99-053
  29. Miller, G. L. 1959. Use of dinitrosalicylic acid as reagent for the determination of reducting sugars. Anal. Chem. 31:426-428 https://doi.org/10.1234/12345678
  30. Ministry of Agriculture and Forestry of Korea. 2002. Korean feeding standard for Korean cattle Hanwoo (Ed. K. N. Kim). Ministry of Agriculture and Forestry of Korea. Seoul. Korea
  31. Nocek, J. E. and J. B. Russell. 1988. Protein and energy as an integrated system: relationship of ruminal protein and carbohydrate availability to microbial synthesis and milk production. J. Dairy Sci. 71:2070-2107 https://doi.org/10.3168/jds.S0022-0302(88)79782-9
  32. Nolan, J. V. and R. A. Leng. 1972. Dynamic aspects of ammonia and urea metabolism in sheep. Br. J. Nutr. 27:177-194 https://doi.org/10.1079/BJN19720081
  33. Ooshima, H., M. Sakata and Y. Harano. 1986. Enhancement of enzymatic hydrolysis of cellulose by surfactant. Biotechnol. Bioeng. 28:1727-1734 https://doi.org/10.1002/bit.260281117
  34. Park, J. W., Y. Takahata, T. Kajiuchi and T. Akehata. 1992. Effects of nonionic surfactant on enzymatic hydrolysis of used newspaper. Biotechnol. Bioeng. 39:117-120 https://doi.org/10.1002/bit.260390117
  35. Plascencia, A., M. A. Lopez-Soto, M. F. Montano, J. G. Serrano, R. A. Ware and R. A. Zinn. 2007. Influence of surfactant supplementation and maceration on the feeding value of rice straw in growing-finishing diets for Holstein steers. J. Anim. Sci. 85:2575-2581 https://doi.org/10.2527/jas.2006-490
  36. Reese, E. T. and A. Maguire. 1969. Surfactants as stimulants of enzyme production by microorganisms. Appl. Mictrobiol. 17:242-245 https://doi.org/10.1016/0960-8524(95)00083-6
  37. Rocch-Ramel, F. 1967. An enzymic and fluorophotometric method for estimating urea concentrations in nanoliter specimens. Anal. Biochem. 21:372-381 https://doi.org/10.1016/0003-2697(67)90312-0
  38. SAS Institute. 2002. $SAS^{\circledR}$ user's guide: Statistics. Version 9.1 Edition. Statistical Analysis Systems Institute Inc., Cary, NC
  39. Satter, L. D. and L. L. Slyter. 1974. Effect of ammonia concentration on rumen microbial protein production in vitro. Br. J. Nutr. 32:199-208 https://doi.org/10.1079/BJN19740073
  40. Shelford, J. A., K. J. Cheng and G. M. Kamande. 1996. Enzyme enhancers: the key to unlocking the energy from feed. In: Advances in dairy technology. Western Canada Dairy Seminar, University of Alberta, Edmonton, AB, Canada. 8:269-275
  41. Stewart, C. S. 1977. Factors affecting the cellulolytic activity of rumen contents. Appl. Environ. Microbiol. 33:497-502
  42. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597 https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  43. Wang, Y., D. Greer and T. A. McAllister. 2003a. Effects of moisture, roller setting and saponin based surfactant on barley processing, ruminal degradation of barley, and growth performance by feedlot steers. J. Anim. Sci. 81:2145-2154
  44. Wang, Y., T. A. McAllister, J. Baah, R. Wilde, K. A. Beauchemin, L. M. Rode, J. A. Shelford, G. M. Kamande and K. J. Cheng. 2003b. Effects of Tween 80 on in vitro fermentation of silages and interactive effects of Tween 80, monensin and exogenous fibrolytic enzymes on growth performance by feedlot cattle, Asian-Aust. J. Anim. Sci. 16:968-978
  45. Wang, Y., T. W. Alexander and T. A. McAllister. 2004. In vitro effects of monensin and Tween 80 on ruminal fermentation of barley grain:barley silage based diets for beef cattle. Anim. Feed Sci. Technol. 116:197-209 https://doi.org/10.1016/j.anifeedsci.2004.06.006
  46. Weimer, P. J., J. M. Lopez-Guisa and A. D. French. 1990. Effect of cellulose fine structure on kinetics of its digestion by mixed ruminal microflora. Appl. Environ. Microbiol. 56:2421-2429

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