BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Microplate hybridization assay for detection of isoniazid resistance in Mycobacterium tuberculosis

  • Han, Hye-Eun (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Lee, In-Soo (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Hwang, Joo-Hwan (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Bang, Hye-Eun (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Kim, Yeun (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Cho, Sang-Nae (Department of Microbiology, Yonsei University College of Medicine) ;
  • Kim, Tae-Ue (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Lee, Hye-Young (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
  • Published : 2009.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Early and accurate detection of drug resistant Mycobacterium tuberculosis can improve both the treatment outcome and public health control of tuberculosis. A number of molecular-based techniques have been developed including ones using probe molecules that target drug resistance-related mutations. Although these techniques are highly specific and sensitive, mixed signals can be obtained when the drug resistant isolates are mixed with drug susceptible isolates. In order to overcome this problem, we developed a new drug susceptibility test (DST) for one of the most effective anti-tuberculosis drug, isoniazid. This technique employed a microplate hybridization assay that quantified signals from each probe molecule, and was evaluated using clinical isolates. The evaluation analysis clearly showed that the microplate hybridization assay was an accurate and rapid method that overcame the limitations of DST based on conventional molecular techniques.

Keywords

References

  1. Wenjun, S., Jianfang, F., Min, Z., Xuhui, L., Shengfeng, X., Xuelian, Z. and Honghai, W. (2007) Biosynthesis of isoprenoids: characterization of functionally active recombinant 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase(IspD) from mycobacterium tuberculosis H37Rv. J. Biochem. Mol. Biol. 40, 911-920 https://doi.org/10.5483/BMBRep.2007.40.6.911
  2. Soini, H. and Musser, J. M. (2001) Molecular diagnosis of mycobacteria. Clin. Chem. 47, 809-814
  3. Cole, S. and Telenti, A. (1995) Drug resistance in Mycobacterium tuberculosis. Eur. Respir. J. 20(Suppl. 9), S701-713
  4. Pfyffer, G. E. (2000) Drug-resistant tuberculosis: resistance mechanisms and rapid susceptibility testing. Schweiz. Med. Wonchenschr. 49, 1909-1913
  5. Ramaswamy, S. and Musser, J. M. (1998) Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuberc. Lung Dis. 79, 3-29 https://doi.org/10.1054/tuld.1998.0002
  6. Zhang, S. L., Shen, J. G., Xu, P. H., Li, D. X., Sun, Z. Q., Li, L., Yang, Z. R. and Sun, Q. (2007) A novel genotypic test for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates by a multiplex probe array. J. Appl. Microbiol. 103, 1262-1271 https://doi.org/10.1111/j.1365-2672.2007.03350.x
  7. Marttila, H. J., Soini, H. and Eerola, E. (1998) A Ser315Thr substitution in katG is predominant in genetically heterogeneous multidrug-resistant Mycobacterium tuberculosis isolates originating from the St Petersburg area in Russia. Antimicrob. Agents Chemother. 42, 2443-2445
  8. Ramaswamy, S. and Musser, J. M. (1998) Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuberc. Lung Dis. 79, 3-29 https://doi.org/10.1054/tuld.1998.0002
  9. Zhang, M., Yue, J., Yang, Y. P., Zhang, H. M., Lei, J.Q., Jin, R. L., Zhang, X. L. and Wang, H. H. (2005) Detection of mutations associated with isoniazid resistance in Mycobacterium tuberculosis isolates from China. J. Clin. Microbiol. 43, 5477-5482 https://doi.org/10.1128/JCM.43.11.5477-5482.2005
  10. Parish, T. and Stoker, N. G. (2001) Mycobacterium tuberculosis protocols, Humana Press, New Jersey, USA

Cited by

  1. High resolution melting curve assay for rapid detection of drug-resistant Mycobacterium tuberculosis vol.19, pp.6, 2013, https://doi.org/10.1007/s10156-013-0636-3