Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Physiological Activities of Roots Extracts from Calystegia japonica

  • Lee, Yang-Suk (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Choi, Bok-Dong (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Joo, Eun-Young (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Kim, Nam-Woo (Department of Herbal Biotechnology, Daegu Haany University)
  • Published : 2009.12.31
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Abstract

This study was investigated to evaluate the contents of polyphenols and flavonoids, and physiological activities of various extracts from Calystegia japonica roots for making good use of their functional materials. The roots of C. japonica were extracted with water (WE), ethanol (EE) and hot water (HWE) by different methods. Among these extracts, the highest extracting yield was 30.30% of HWE, while the highest contents of total polyphenols and flavonoids were 40.85% and 6.40% of WE, respectively. The nitrite scavenging abilities were ranged from 31.31% (HWE) to 37.46% (EE) at pH 1.2 and 1.0 mg/ml concentration. In the measurements of electron donating abilities, EE showed the highest effect as 91.83% at 0.3 mg/ml assay concentration, and the electron donating ability was decreased as the extract concentration was increased. In the results of superoxide dismutase (SOD)-like activity, HWE showed the highest effect as 7.15% at 1.0 mg/ml. The tyrosinase inhibition activities of WE and EE were 15.28% and 14.97%, respectively. The xanthine oxidase inhibitory effects were ranged from 97.50 to 99.28% at 1.0 mg/ml. These results indicate that C. japonica extract has a good antioxidant effects and could be useful for developing functional products.

Keywords

References

  1. AOAC. Official method of analysis. 18th ed., 2005. 45: 21-22. Association of official analytical chemists. Washington D.C. USA.
  2. Aruoma OI. Nutrition and health aspects of free radicals and antioxidants. Food Chem Toxicol. 1994. 32: 671-683. https://doi.org/10.1016/0278-6915(94)90011-6
  3. Bell AA, Weeler MH. Biosynthesis and melanin. Ann Rev Phytophathol. 1986. 24: 411-451. https://doi.org/10.1146/annurev.py.24.090186.002211
  4. Blois MS. Antioxidant determination by the use of a stable free radical. Nature 1958. 181: 1199-1200. https://doi.org/10.1038/1811199a0
  5. Branen AL. Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc. 1975. 52: 59-63. https://doi.org/10.1007/BF02901825
  6. Donnelly JK, McLellan KM, Walker JL, Robinson DS. Superoxide dismutases in foods. A review Food Chem. 1989. 33: 243-270. https://doi.org/10.1016/0308-8146(89)90036-8
  7. Freeman BA, Grapo JD. Biology of disease; free radicals and tissue injury. Lab Invest. 1982. 47: 412-426.
  8. Halliwell B. Antioxidants in human health and disease. Ann Rev Nutr. 1996. 16: 33-50. https://doi.org/10.1146/annurev.nu.16.070196.000341
  9. Hong SP, Jeong HS, Jeong EJ, Shin DH. Quality characteristic of beverage with Gastrodia elata blume extract. J Food Hyg Safety 2006. 21: 31-35.
  10. Joo EY, Kim NW. Polyphenol contents and antioxidant activity of extracts from Angelica dahurica root after different conditions of microwave-assisted extraction. Korean J Food Preser. 2008. 15: 133-138.
  11. Kandaswami C, Middleton EJ. Free radical scavenging and antioxidant activity of plant flavonoids. In: Free radicals in diagnostic medicine. 1994. pp. 351-376. Armstrong D (de). Plenum Press, New York and London.
  12. Kang MJ, Shin SR, Kim K. Antioxidative and free radical scavenging activity of water extract from Dandelion (Taraxacum officinale). Korean J Food Preser. 2002. 9: 253-259.
  13. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem. 1987. 51: 1333-1338. https://doi.org/10.1271/bbb1961.51.1333
  14. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. Antioxidative activity and physiological activity of some Korean medical plants. Korean J Food Sci Technol. 1995. 27: 80-85.
  15. Kim YS, Choi BY. Chromosome number, morphological and anatomical study on Calystegia in Korea. Kor J Plant Tax. 1983. 13: 89-107. https://doi.org/10.11110/kjpt.1983.13.2.089
  16. Kuhnau J. The flavonoide; A calss of semiessential food components; Their role in human nutrition. World Rev Nutr Diet. 1976. 24: 117-120.
  17. Labuza TP. Kinetic of lipid oxidation in foods. CRC Rev Food Technol. 1971. 2: 335.
  18. Lee BY, Hwang JB. Physicochemical characteristics of Agastache rugosa O. Kuntze extracts by extraction conditions. Korean J Food Sci Technol. 2000. 32: 1-8.
  19. Lee MS, Choi HS. Volatile flavor components in various edible portions of Calystegia japonica (THUNB) CHOIS. Korean J Food Sci Technol. 1994. 26: 359-364.
  20. Lee TB. Illustrated Flora of Korea. 5th ed. 1993. p 635. Hyangmoonsa, Seoul, Korea.
  21. Marklund S, Marklund G. Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1975. 47: 468-474.
  22. Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol. 2000. 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  23. Oh YC, Lee CS, Park EJ. A chemotaxonomic study on the genus Calystegia (Convolvulaceae) in Korea. Kor J Plant Tax. 1995. 25: 13-24. https://doi.org/10.11110/kjpt.1995.25.1.013
  24. Park. YB, Lee TG, Kim OK, Do JR, Yeo SG, Park YH, Kim SB. Characteristics of nitrite scavenger derived from seeds of Cssia tora L. Korean J Food Sci Technol. 1995. 27: 124-128.
  25. Peter FS. The toxicology of nitrate, nitrite and N-nitroso compounds. J Sci Food Agric. 1975. 52: 1761-1764.
  26. Sanchez-Ferer A, Rodriguez-Lopez JN, Garcia-Canova F, Garcia-Carmona F. Tyrosinase a comprehensive review of its mechanism. Biochem Biophys Acta. 1995. 1247: 1-11. https://doi.org/10.1016/0167-4838(94)00204-T
  27. Shenoy NR, Choughuley ASU. Effect of certain phenolics on nitrosamine formation. J Agric Food Chem. 1989. 37: 721-725. https://doi.org/10.1021/jf00087a031
  28. Stirpe F, Corte ED. The regulation of rat liver xanthine oxidase. J Biol Chem. 1969. 244: 3855-3861.
  29. Stoner GD, Mukhtar H. Polyphenols as cancer chemopreventive agents. J Cell Bio Chem. 1995. 22: 169-180.
  30. Storch I, Ferber E. Detergent-amplified chemiluminescence of lucigenin for determination of superoxide amino production by NADPH oxidase and xanthine oxidase. Anal Biochem. 1988. 169: 262-267. https://doi.org/10.1016/0003-2697(88)90283-7
  31. Takashi Y, Yamamoto M, Tamura A. Studies on the formation of nitrosamines; The effects of some polyphenols on nitrosation of diethylamine. J Food Hyg Soc. 1978. 19: 224-229. https://doi.org/10.3358/shokueishi.19.224
  32. Walker EA, Pignatelli B, Friensen M. The role of phenols in catalysis of nitrosamine formation. J Sci Food Agric. 1982. 33: 81-86. https://doi.org/10.1002/jsfa.2740330115
  33. Yagi A, Kanbara T, Morinobu N. Inhibition of mushroomtyrosinase by aloe extract. Planta Med. 1987. 53: 517-519. https://doi.org/10.1055/s-2006-962799