Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Herbal Sports Drinks with Omija, Maesil and Molasses on the Endurance and Energy Metabolites of Experimental Animals

오미자, 매실 및 당밀을 함유하는 한방스포츠음료가 실험동물의 지구력과 에너지 대사 성분에 미치는 영향

  • Bachri, Saiful (Department of Pharmacy, Kyungsung University) ;
  • Woo, Mi-Hee (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Lee, Hang-Woo (Daegu-Gyungbuk Institute for Oriental Medicine Industry) ;
  • Choi, Jong-Won (Department of Pharmacy, Kyungsung University) ;
  • Kim, Hee-Sook (Department of Food Science and Biotechnology, Kyungsung University)
  • Published : 2009.02.28
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Abstract

The antifatigue and endurance promoting properties of two Korean medicinal herb extracts and molasses with various mineral components were studied by evaluating forced-swimming capacity and biochemical parameters in ICR mice. The treatment groups were orally administered mineral beverages which were contained 6% sugar with the mixture of Maesil (Prunus mume fruit) extracts, Omija (Schisandra chinensis fruit) extracts and molasses for 4 weeks. The exercised forced-swimming tests were conducted after 28 days of beverage supplementation. The swimming times to exhaustion were longer 1.5${\sim}$2 times in group 6 and group 10 than control goup (Control: 93.2${\pm}$10.4 sec; Beverage 6; 190.8${\pm}$25.6 sec, Beverage 10; 173.6${\pm}$21.8 sec; p<0.05). Moreover, the activity of hexokinase (Control: 5.23${\pm}$0.38 ${\mu}mol$l/g tissue; Beverage 6: 5.99${\pm}$0.18 ${\mu}mol$/g tissue, Beverage 10: 6.13${\pm}$0.25 ${\mu}mol$/g tissue, p<0.05) and citrate synthase (control: 42.9${\pm}$1.87 ${\mu}mol$/g tissue; Beverage 6: 56.8${\pm}$3.98 ${\mu}mol$/g tissue, Beverage 10; 59.5${\pm}$3.09 ${\mu}mol$/g tissue, p<0.05) were also significantly higher than those of control group. Even if the treatment groups had long swimming than control group, there is no significant difference in the glycogen contents of gastrocnemus muscle or liver between the control group and each treatment group. This demonstrated an improvement in endurance. These results suggest that reported herbal beverage is very effective to combat fatigue, improve endurance and increase overall physical activity.

한방재료 즉 오미자 및 매실 추출물과 당밀이 혼합된 미네랄 음료의 항피로효과 및 지구력 증진효과를 연구하기 위하여 ICR 생쥐의 강제수영능력 및 생화학적 요인을 측정하였다. 6% 당과 전해질용액에 오미자추출물, 매실추출물 및 당밀을 혼합한 음료들을 실험군들에게 4주 동안 자유로이 섭취시켰다. 음료들을 섭취한 10 group의 실험동물들은 28일 후 강제수영하였으며 한방음료를 섭취한 실험군(6번 음료; 190.8${\pm}$25.6 sec, 10번 음료; 173.6${\pm}$21.8 sec; p<0.05)의 강제수영시간은 대조군(93.2${\pm}$10.4 sec)에 비하여 1.5${\sim}$2배 증가하였다. 또한 6번 음료 및 10번 음료의 hexokinase 및 citrate synthase 활성 역시 대조군에 비하여 유의적으로 높았다 (hexokinase 활성 - 대조군:5.23${\pm}$0.38 ${\mu}mol$/g tissue; 6번 음료: 5.99${\pm}$0.18 ${\mu}mol$l/g tissue, 10번 음료: 6.13${\pm}$0.25 ${\mu}mol$/g tissue, p<0.05, citrate synthase 활성 - (대조군: 42.9${\pm}$1.87 ${\mu}mol$/g tissue; 6번 음료: 56.8${\pm}$3.98 ${\mu}mol$/g tissue, 10번 음료; 59.5${\pm}$3.09 ${\mu}mol$/g tissue, p<0.05). 한방음료를 섭취한 실험군 이 대조군에 비하여 훨씬 오랜 시간 강제수영을 하였으나 비복근 및 간의 glycogen 함량은 실험동물군들 사이에서 유의적인 차이가 나타나지 않았다. 이것은 지구력이 증진되었음을 보여주는 한 예이다. 이러한 실험결과는 실험에 사용한 한방음료가 피로예방, 지구력 증진 및 운동수행능력을 증진하는데 매우 효과가 있음을 말해준다.

Keywords

References

  1. Azevedo, J., L. Jr, J. K. Linderman, S. L. Lehman, and G. A. Brooks. 1998. Training decreases muscle glycogen turnover during exercise. Eur. J. Appl. Physiol. Occup. Physiol. 78, 479-486 https://doi.org/10.1007/s004210050449
  2. Banister, E. W., W. Rajendra, and B. J. Mutch. 1985. Ammonia as an indicator of exercise stress implications of recent findings to sports medicine. Sports Med. 2, 34-46 https://doi.org/10.2165/00007256-198502010-00004
  3. Beltz, S. D. and P. L. Doering. 1993. Efficacy of nutritional supplements used by athletes. Clin. Pharm. 12, 900-908
  4. Bigard, A. X., A. Brunet, C. Y. Guezennec, and H. Monod. 1991. Skeletal muscle changes after endurance training at high altitude. J. Appl. Physiol. 71, 2114-2121
  5. Calfee, R. and P. Fadale. 2006. Popular ergogenic drugs and supplements in young athletes. Pediatrics 117, e557-e589 https://doi.org/10.1542/peds.2005-1597
  6. Chuda, Y., H. Ono, M. Ohnishi-Kameyame, K. Matsumoto, T. Nagata, and Y. Kikuchi. 1999. Mumefral, citric acid derivative improving blood fluidity from fruit-juice concentrate of Japanese apricot. J. Agric. Food Chem. 47, 828-831 https://doi.org/10.1021/jf980960t
  7. Choi, Y. J., J. K. Oh, and G. S. Ahn. 2007. Functional food for sports. Korea Patent 10-0714464
  8. Coleman, E. 1991. Study of sports drinks. Food and Packaging 32, 584-586
  9. Coyle, E., A. Coogan, M. Hemmert, and J. Ivy. 1986. Muscle glycogen utilization during prolonged strenuous exercise when fed carbohydrate. J. Appl. Physiol. 61, 165-172
  10. Crill, C. M. and R. A. Helms. 2007. The use of carnitine in pediatric nutrition. Nutr. Clin. Pract. 22, 204-213 https://doi.org/10.1177/0115426507022002204
  11. Dawson, C. A., E. R. Nadel and S. M. Horvath. 1971. Arterial blood and muscle lactates during swimming in the rat. J. Appl. Physiol. 30, 322-327 https://doi.org/10.1016/0031-9384(94)90226-7
  12. Delamarche, P., M. Monnier, A. Gratas-Delamarche, H. E. Koubi, M. H. Mayet, and R. Favier. 1992. Glucose and free fatty acid utilization during prolonged exercise in prepubertal boys in relation to catecholamine responses. Eur. J. Appl. Physiol. Occup. Physiol. 65, 66-72 https://doi.org/10.1007/BF01466276
  13. Gleim, G. G. and B. Glace. 1998. Carnitine as an ergogenic aid in health and disease. J. Am. Coll. Nutr. 17, 203-204 https://doi.org/10.1080/07315724.1998.10718748
  14. Han, J. W. and S. H. Park. 2006. produced by using medicinal herbs with a usage as recovery of exhaustion. Korea Patent 10-0637700
  15. Hikino, H., Y. Kiso, H. Taguchi, and Y. Ikeya. 1984. Antihepatotoxic actions of lignoids from Schizandra chinensis fruits. Planta Med. 50, 213-218 https://doi.org/10.1055/s-2007-969681
  16. Hong, S. -G., D. -S. Yang, B. -J. Kang, H. -S. Lee, and Y. Yoon. 2003. Effects of formula (JR-22) maybe containing traditional herbs on maximal exercise performance and antioxidant meterials in murine model. J. Korean Soc. Food Sci. Nutr. 32, 1076-1081 https://doi.org/10.3746/jkfn.2003.32.7.1076
  17. Itaya, K. and M. Ui. 1966. A new micromethod for the colorimetric determination of organic phosphate. Clin. Chim. Acta 14, 361-366 https://doi.org/10.1016/0009-8981(66)90114-8
  18. Joshi, M. D. and V. Japannathan. 1969. Hexokinase. Methods in enzymology 13, 371-381
  19. Jung, K., I. -H. Kim, and D. Han. 2004. Effect of medicinal plant extracts on forced swimming capacity in mice. J. Ethnopharmacol. 93, 75-81 https://doi.org/10.1016/j.jep.2004.03.022
  20. Khanna, G. L. and I. Manna. 2005. Supplementary effect of carbohydrate-electrolyte drink on sports performance, lactate removal and cardiovascular response of athletes. Indian J. Med. Res. 121, 665-669
  21. Kim, K. M., K. W. Tu, D. H. Kang, and H. J. Suh. 2002. Anti-stress and anti-fatigue effect of fermented rice bran. Phytotheraphy Res. 16, 700-702 https://doi.org/10.1002/ptr.1019
  22. Kim, H. S. 2008. Development of herbal sports drinks for the anti-fatigue and exercise endurance. 2008 BTP Report pp. 49
  23. Kvasnickova, L., Z. Glatz, H. Sterbova, V. Kahle, J. Slanina, and P. Musil. 2001. Application of capillary electrochromatography using macroporous polyacrylamide cloumns for the analysis of lignans from seeds of Schisandra chinensis. J. Chromatography A 916, 265-271 https://doi.org/10.1016/S0021-9673(01)00556-8
  24. Koo, H. -N., J. -K. Lee, S. -H. Hong, and H. -M. Kim. 2004. Herbkines increases physical stamina in mice. Biol. Pharm. Bull. 27, 117-119 https://doi.org/10.1248/bpb.27.117
  25. Laos, C. and J. D. Metzl. 2006. Performance-enhancing drug use in young athletes. Aldolesc. Med. Clin. 17, 716-731
  26. Leichtweis, S. B., C. Leeuwenburgh, D. J. Parmelee, R. Fiebig, and L. L. Ji. 1997. Rigorous swim training mitochondrial function in post-ischaemic rat heart. Acta Physiol. Scand. 160, 139-148 https://doi.org/10.1046/j.1365-201X.1997.00138.x
  27. Matsumoto, K., H. Hayase, K. Imaizumi, H. Tsujimoto, and R. Mitsuzono. 2007. Branched-chain amino acids supplementation attenuates the accumulation of blood lactate dehydrogenase during distance running. J. Sports Med. Phys. Fitness 47, 316-322
  28. Opletal, L., H. Sovová, and M. Bártlová. 2004. Dibenzo [a,c]cyclooctadiene lignans of the genus Schisandra: importance, isolation and determination. J. Chromatography B 812, 357-371
  29. Park, T. S. 2005. The annual report of KFDA. Korean Food Drug Admin. 9. 467-468
  30. Pesce, A., T. P. Fondy, F. Stolzenbach, F. Castillo, and N. O. Kaplan. 1967. Comparative enzymology of lactate dehydrogenase. J. Biol. Chem. 242, 2151-2167
  31. Schenker, S, D. W. McCandless, E. Brophy, and M. S. Lewis. 1967. Studies on the intercerebral toxicity of ammonia. J. Clin. Invest. 46, 838-848 https://doi.org/10.1172/JCI105583
  32. Soh, D. M., E. K. Won, K. S. Choi, J. S. Park, N. I. Kim, Y. I. Kim, J. J. Lee, and D. S. Yeon. 2000. Effects of L-carnithine containing electrolyte beverages on endurance performance and glycogen sparing effects of trained rats. Korean J. Sports Med. 18, 314-324
  33. Srere, P. A. 1969. Citrate synthetase. Methods in enzymology 13, 3-11 https://doi.org/10.1016/0076-6879(69)13005-0
  34. Tanaka, K., K. Koga, T. Kawamura, K. Kawabata, T. Soejima, T. Endoh, T. Bandoh, K. Fukuda, N. Kanai, and M. Sakakibara. 2004. Evaluation of the effects of japanese apricot extracts on blood fluidity by means of MC-FAN. J. Jpn. Soc. Nutr. Food Sci. 57, 81-87 https://doi.org/10.4327/jsnfs.57.81
  35. Taylor, J. C., L. Rapport, and G. B. Lockwood. 2003. Octacosanol in human health. Nutrition 19, 192-195 https://doi.org/10.1016/S0899-9007(02)00869-9
  36. Von Duvillard, S. P., W. A. Braun, M. Markofski, R. Beneke, and R. Leithäuser. 2004. Fluids and hydration in prolonged endurance performance. Nutrition 20, 651-656
  37. Williams, M. H. 2005. Dietary supplements and sports performance: minerals. J. Int. Soc. Sports Nutr. 11, 43-49 https://doi.org/10.1186/1550-2783-2-1-43
  38. Yang, D. C. 2005. The annual report of KFDA. Korean Food Drug Admin. 9, 456-457

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