Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Cheonggukjang Added Phellinus linteus myceria on Lipid Metalbolism in Adult Female Rats

상황버섯균사체청국장이 고지혈증을 유도한 암쥐의 지질대사에 미치는 영향

  • Choi, Mi-Ae (Dept. of Hotel Culinary Arts, Yansan college)
  • 최미애 (양산대학 호텔조리계열)
  • Published : 2009.11.30
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Abstract

This study was performed to investigate the effects of Cheonggukjang (traditional soybean food, CK) and Cheonggukjang added with Phellinus linteus myceria (CKP) on the lipid metabolism, growth, food intake, and food efficiency ratio(FER) in adult female rats. Sprague-Dawley rats were fed a high fat diet (control diet: 0.1% cholesterol, 10% Lard) for 4 weeks. The rats were randomly assigned to each treatment group: control, and two kinds of CK and CKP (powders of CK and CKP as dietary protein sources). After 4 weeks of experimental diet consumption, the body weights and the uterine fat pad weights of the CK and CKP diet groups were more significantly decreased than those of the control diet group. The hepatic cholesterol, triglyceride and total lipid levels were significantly lower in both the CK and CKP diet fed groups than those in the control group. The concentrations in serum triglyceride and LDL-cholesterol were significantly decreased in the CK and CKP diet groups compared to those in the control group. Fecal total lipid, moisture and wet weight excretion in the CK and CKP diet groups were more increased than those in the control group. These results showed that feeding of cheonggukjang and cheonggukjang added with Phellinus linteus myceria both the triglyceride and LDL-cholesterol in serum, as well as the triglyceride and cholesterol in the livers of the rats.

청국장 분말이 고지방식이를 섭취한 흰쥐의 지질대사에 미치는 영향을 조사하고자 성숙한 암쥐에 4주간 고지방식이 (0.1% 콜레스테롤, 10% 지방)로 고지혈증을 유도한 후 대조군(고지방 식이), 청국장군(고지방식이에 단백질원으로 청국장 분말을 첨가한 식이) 및 상황버섯균사체 청국장군(고지방 식이에 단백질원으로 상황버섯균사체 청국장 분말을 첨가한 식이) 3군으로 나누어 4주간 사육한 결과는 다음과 같다. 실험동물의 체중변화는 대조군은 15.99 g의 증가에 비해 청국장군 및 상황버섯 청국장군은 각각 4.47 g 및 1.89 g 증가로 유의하게 감소되었고 식이섭취량 및 식이효율도 대조군에 비해 청국장군들이 감소하는 경향을 나타내었다. 자궁주위 지방의 무게 및 간조직의 콜레스테롤과 중성지질 농도는 대조군에 비해 청국장군 및 상황버섯균사체 청국장군이 유의하게 감소하였으며 혈청의 중성지질, 총 콜레스테롤, LDL-콜레스테롤 농도 및 AI는 대조군에 비해 청국장군 및 상황버섯균사체 청국장군이 유의하게 감소되었고, 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율은 대조군에 비해 청국장군들이 유의하게 증가되었다. 변의 무게, 수분함량 및 총지질 배설량은 청국장군들이 대조군 보다 유의하게 증가된 것으로 나타났다. 이상의 결과로 보아 고지방식이에 청국장 및 상황버섯균사체 청국장 분말 첨가 섭취시 흰쥐의 체중감소와 혈청과 간조직의 중성지질, 총 콜레스테롤과 LDL-콜레스테롤 농도 및 동맥경화지수를 낮추며 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율이 증가되어 지질대사의 개선 효과가 있는 것으로 나타났다.

Keywords

References

  1. Anderson, J. W., D. A. Deakins, T. L. Floore, B. M. Smith, and S. E. Whitis. 1990. Dietary fiber and coronary heart disease. Crit. Rev. Food Sci. Nutr. 29, 95-147 https://doi.org/10.1080/10408399009527518
  2. AOAC. 1980. Office of analysis 15th ed Association of Official Analytical Chemistry. 211-260
  3. Assmann, G. and H. Schulth. 1992. Relation of HDL-cholesterol and triglyceride to incidence of atherosclerotic coronary artery disease (The PROCAM Experimence). Am. J. Cardiol. 70, 733-737 https://doi.org/10.1016/0002-9149(92)90550-I
  4. Byun, M. W., J. H. Son., H. S. Yook., C. Jo, and D. H. Kim. 2002 Effect of gamma irradiation on the physiological activity of Korean soybean, fermented foods, Chungkookjang and Doenjang. Radiation Physics and Chemistry 64, 245-248 https://doi.org/10.1016/S0969-806X(01)00492-3
  5. Choi, Y. S. and S. Y. Lee. 1992. Review: Serum cholesterol and 3-hydroxy-3-methylglutaryl coenzyme A reductase. J. Korean Soc. Food Nutr. 21, 580-593
  6. Davidson, I. and J. B. Henry. 1996. Todd-Sanford clinical diagnosis by labora-tory methods, 13th eds. EB Saunders Co Philadelphia 73
  7. Frings, C. S. and R. T. Dunn. 1970. A colorimetric method for determination of total serum lipid based on the sulfophospho-vanillin reaction. Am. J. Clin. Path. 89-91 https://doi.org/10.1016/S0167-7012(03)00193-3
  8. Haglund, O., R. Loustarinen, R. Wallin, I. Wibell, and T. Saldeen. 1991. The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin. Eur. J. Nutr. 121, 165-172
  9. Huff, M. W. and K. K. Carrol. 1980. Effect of dietary protein and amino acid mixtures on plasma cholesterol level in rabbits. J. Nutr. 110, 1676-1685
  10. Inkeles, S. and D. Isenberg. 1981. Hyperlipidedia and coronary atherosclerosis. Medicine (Baltimore) 60, 110-123
  11. Kim, J. I., M. J. Kang, and T. W. Kwon. 2003. Antidiabetic effect of soybean and chongkukjang. Korea Soybean Digest 20, 44-52
  12. Kim, S. C., S. H. Lee, and S. J. Wi. 2002. The effect of natto mucilage on the components of serum lipid in rats. J. Korean Oil Chemists Soc. 19, 63-67
  13. Koh, J. B. 2006. Effects of cheonggukjang added Phellinus linteus on lipid metabolism in hyperlipidemic rats. J. Korean Soc. Food Sci. Nutr. 35, 410-415 https://doi.org/10.3746/jkfn.2006.35.4.410
  14. Kritchevsky, D. 1976. Diet and atherosclerosis. Am .J. Pathol. 84, 615-632
  15. Lee, B. Y., D. M. Kim, and K. H. Kim. 1991. Physico chemical properties of viscous substance extracted from chongkookjang. Korean J. Food Sci. Technol. 25, 64-67
  16. Lee, H. J. and J. B. Koh. 2003. Effect of liquid culture of Agaricus blazei Murill on lipid metabolism and enzyme activities in rats fed high fat diet. Korean J. Nutr. 36, 352-258
  17. Mannienen, V., L. Tenkanen., P. Koskinen, J. K. Hutlunen, C. Manntari, O. P. Heinonen, and M. H. Frck. 1992. Triglycerides and LDL-cholesterol and HDL-cholesterol concentrations on coronary heart disease risk in the Helsinki heart study. Circulation 85, 37-45 https://doi.org/10.1161/01.CIR.85.1.37
  18. Ministry of Health and Welfare Republic of Korea. 2005. Year book of Health and Welfare Statistics. 51-77
  19. Okita, T. 1989. Hypocholesterolemic effects of soy protein. Nutr. Sci. Soy Protein Jpn. 10, 58-62
  20. Park, M. S. and G. U. Liepa. 1982. Effect of dietary protein and amino acid on the metabolism of cholestreol carring lipoproteins in rats. J. Nutr. 112, 1892-1898 https://doi.org/10.1016/S0271-5317(05)80511-1
  21. Park, Y. H., J. S. Lee, and Y. J. Lee. 1993. Distribution of serum lipid by age and the relation of serum lipid to degree of obesity and blood pressure in Korean adults. Kor. J. Lipidiology 3, 165-180
  22. Reeves, P. G., F. H. Nielsen, and G. C. Fahey. 1993. AIN-93 purified diets for laboratory rodents final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123, 1939-1951
  23. Sautier, C., C. Doucet, C. Flament, and D. Lemonnier. 1979. Effect of soy protein and saponins on serum, tissue and feces steroids in rats. Atherosclerosis 34, 233-241 https://doi.org/10.1016/S0021-9150(79)80004-0
  24. Song, C. W., H. S. Hwang, and S. S. Han. 1990. Studies on the basic data of Ktc: SD rats with age. Korean J. Lab. Ani. Sci. 6, 33-43
  25. Sumi, H., H. Hamada, H. Tsushima, H. Mihara, and H. Muraki. 1987. A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese, natto: a typical and popular soy bean food in the Japanese diet. Experientia 43, 1110-1111 https://doi.org/10.1007/BF01956052
  26. Taguchi, K., M. Kawabata, K. Ohtsuki, and Y. Tanaka. 1986. Changes in dietary fiber of natto and tempeh during fermentation. J. Jpn. Soc. Nutr. Food Science 39, 203-208 https://doi.org/10.4327/jsnfs.39.203
  27. Yang, J. L., S. H. Lee, and Y. S. Song. 2003. Improving effect of powders of cooked soybean and chongkukjang on blood pressure and lipid metabolism in spontaneously hypertensive rats. J. Koran Soc. Food Sci. Nutr. 32, 899-905 https://doi.org/10.3746/jkfn.2003.32.6.899
  28. Yokota, T., T. Hattori, H. Ohishi, K. Hasegawa, and K. Watanabe. 1987. The effect of anti oxidant containing fraction from fermented soybean food on atherosclerosis development in cholesterol metabolism in immature and mature rat. J. Nutr. 117, 30-35

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