Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Pretreatment of Cane Molasses for Production of Bacterial Cellulose and Its Physico-Chemical Properties

미생물 셀룰로오스 생산을 위한 당밀의 전처리 및 생산된 셀룰로오스의 물리화학적 특성

  • Jung, Ho-Il (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Jeong, Jin-Ha (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Jeon, Young-Dong (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Lee, Na-Ri (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Park, Ki-Hyun (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Kim, Yong-Gyun (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Park, Geun-Tae (Research & University-Industry Cooperation, Pusan National University) ;
  • Son, Hong-Joo (Department of Life Science & Environmental Biochemistry, Pusan National University)
  • 정호일 (부산대학교 생명환경화학과) ;
  • 정진하 (부산대학교 생명환경화학과) ;
  • 전영동 (부산대학교 생명환경화학과) ;
  • 이나리 (부산대학교 생명환경화학과) ;
  • 박기현 (부산대학교 생명환경화학과) ;
  • 김용균 (부산대학교 생명환경화학과) ;
  • 박근태 (부산대학교 산학협력단) ;
  • 손홍주 (부산대학교 생명환경화학과)
  • Published : 2009.10.30
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Abstract

The aim of this study is to investigate cane molasses pretreatments for the production of cellulose by Acetobacter sp. V6, which has excellent bacterial cellulose (BC) producing capacity in the shaking culture. Among pretreatments of cane molasses, 1% (w/v) tricalcium phosphate (TP) treatment was more efficient in BC production. The physico-chemical properties of BCs that were produced in static and shaking cultures were also investigated. Although BC had an emulsifying ability, its emulsion stability was low. Water holding capacity (WHC) of BC was high; the WHC of BC produced in static culture was 14 times higher than that of $\alpha$-cellulose. In addition, the viscosity of BC was higher than that of $\alpha$-cellulose. Composition analysis by FT-IR showed no difference in composition between BC and plant cellulose. In the crystallinity analysis by XRD, all BC samples showed crystallinity. All BC samples showed reticulated structures consisting of ultrafine cellulose fibriles. Microfibriles of cellulose from static culture were especially more compact than those of cellulose from shaking culture.

BC 합성능이 우수하며, 진탕배양에서도 BC를 생산할 수 있는 능력이 있음이 확인된 Aectobacter sp. V6로부터 BC 생산을 위한 당밀 전처리 조건을 검토하였으며, 생산된 BC의 물리화학적 특성을 검토하였다. 당밀을 배지성분으로 사용하기 위해 전처리를 실시한 결과, 1%의 TP가 BC 생산에 가장 효율적이었다. 전처리한 당밀배지를 이용하여 정치 및 진탕배양에서 생산된 BC의 물리화학적 특성을 조사한 결과, 모든 BC는 유화능이 있었으나 유화 안정능은 낮았다. 또한 모든 BC에서 높은 수분 보유능이 나타났으며, 특히 정치배양에서 생산된 BC는 $\alpha$-cellulose보다 14배 이상 높았다. BC의 점도는 모두 $\alpha$-cellulose보다 높았으며, 점도계 회전속도의 증가에 따라 급격히 감소하였다. FT-IR을 통한 조성 분석에서 BC는 식물성 셀룰로 오스와 차이가 없었으며, XRD를 통한 결정성 분석에서는 모든 BC가 결정성을 나타내었다. BC의 미세구조를 조사한 결과, 모든 BC가 미세망상구조로 이루어져 있었는데, 이로 인해 BC는 식물성 셀룰로오스와는 달리 독특한 물성을 가지는 것으로 판단되었다. 또한 정치배양에서 생산된 BC는 진탕배양에서 생산된 것보다 셀룰로오스 미세섬유가 조밀하게 얽혀있음을 알 수 있었다.

Keywords

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