The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Samultang on Glutamate-Induced Apoptosis of Hippocampus Cells

사물탕(四物湯)이 Glutamate에 의한 해마세포의 손상에 미치는 영향

  • Jeong, Dae-Young (Department of Internal Medicine, School of Oriental Medicine, Wonkwang University) ;
  • Choi, Chul-Won (Department of Internal Medicine, School of Oriental Medicine, Wonkwang University) ;
  • Moon, Byung-Soon (Department of Internal Medicine, School of Oriental Medicine, Wonkwang University)
  • 정대영 (원광대학교 한의과대학 내과학교실) ;
  • 최철원 (원광대학교 한의과대학 내과학교실) ;
  • 문병순 (원광대학교 한의과대학 내과학교실)
  • Published : 2009.01.30
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Abstract

Objective: This study was designed to investigate the effect of Samultang (SMT) under hippocampus cells ischemia both in vitro and in vivo. Methods: In the in vitro study, HT22 cells, predominantly detected in the cytoplasm, which coincides with the location of the mitochondria, were used as indicators. In the in vivo study, permanent middle cerebral artery occlusion (MCAO) was induced on rats. SMT was given orally 2 h before induction of permanent focal brain ischemic injury. Result: In the in vitro study, SMT had protective effects in glutamate-induced cytotoxicity, which was revealed as apoptosis characterized by chromatic condensation and the loss of mitochondrial membrane potential in HT22 cells. In the in vivo study, TTC (2,3,5-triphenyltetrazolium chloride) staining showed a marked ischemic injury in blood supply territory of the middle cerebral artery (MCA) such as the cerebral cortex and striatum. However, treatment with SMT significantly reduced infarcted volume. SMT increased marked survival of HT22 cells against glutamate-induced cytotoxicity in MTT assay. Conclusion: These results suggest that water extract of SMT provides neuroprotection against ischemic or oxidative injury by inhibition of apoptotic cell death.

Keywords

References

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