DOI QR코드

DOI QR Code

Detection of Fish Killing Dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum (Dinophyceae) in the East China Sea by Real-time PCR

  • Park, Tae-Gyu (Marine Ecology Research Division, National Fisheries Research and Development Institute) ;
  • Kang, Yang-Soon (Marine Ecology Research Division, National Fisheries Research and Development Institute) ;
  • Park, Young-Tae (Marine Ecology Research Division, National Fisheries Research and Development Institute) ;
  • Bae, Heon-Meen (Marine Ecology Research Division, National Fisheries Research and Development Institute) ;
  • Lee, Yoon (Marine Ecology Research Division, National Fisheries Research and Development Institute)
  • Published : 2009.06.01

Abstract

The rDNAs of figh-killing dinoflagellates Cochlodinium polykrikoides and Karlodinium veneficum were detected from the East China Sea by species-specific real-time PCR probes. Sequence analysesusing the partial ITS sequences from the real-time PCR products showed identical sequences with C. Polykrikoides and K. veneficum, respectively and low expectation values (E-value) of less than 1e-5 suggesting the presence of these organisms in the East Ching Sea shelf water that flows into the Tsushima Strait and the Yellow Sea.

Keywords

References

  1. Anderson D.M., Kulis D.M., Doucette G.J., Gallagher J.C. and Balech E. 1994. Biogeography of toxic dinoflagellates in thegenus Alexandrium from the northeastern United States and Canada. Mar. Biol. 120:467-478. https://doi.org/10.1007/BF00680222
  2. Azanza R.V., David L.T., Borja R.T., Baula I. U. and Fukuyo Y. 2008. An extensive Cochlodinium bloom along the western coast of Palawan, Philippines. Harmful Algae 7:324-330. https://doi.org/10.1016/j.hal.2007.12.011
  3. Cho H. and Matsuoka K. 2001. Distribution of dinoflagellatecysts in surface sediments from the Yellow Sea and East China Sea. Mar. Micropaleontol. 42:103-123. https://doi.org/10.1016/S0377-8398(01)00016-0
  4. Coyne K.J., Hare C.E., Popels L.C., Hutchins D.A. and Cary S.C.2006. Distribution of Pfiesteria piscicida cyst populations in sediments of the Delaware Inland Bays, USA. Harmful Algae 5: 363-373. https://doi.org/10.1016/j.hal.2005.07.008
  5. Gong G.C., Chen Y.L.L., Liu K.K. 1996. Chemical hydrographyand chlorophyll distribution in the East China Sea in summer: implications in nutrient dynamics. Cont. Shelf Res. 16:1561-1590. https://doi.org/10.1016/0278-4343(96)00005-2
  6. Guillard R.R.L. and Ryther J.H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea cleve. Can. J. Microbiol. 8: 229-239. https://doi.org/10.1139/m62-029
  7. Guy R.A., Payment P., Krill U.J., Horgen P.A. 2003. Real-timePCR for quantification of Giardia and Cryptospordium in environmental water samples and sewage. Appl. Environ. Microbiol. 69:5178-5185. https://doi.org/10.1128/AEM.69.9.5178-5185.2003
  8. Heath M., Zenitani H., Watanabe Y., Kimura R. and Ishida M1998. Modelling the dispersal of larval Japanese sardine,Sardinops melanostictus, by the Kuroshio Current in 1993 and 1994. Fish. Oceanogr. 7:335-346. https://doi.org/10.1046/j.1365-2419.1998.00076.x
  9. Ichikawa H. and Beardsley R.C. 2002. The current system in the Yellow and East China Seas. J. Oceanogr. 58:77-92. https://doi.org/10.1023/A:1015876701363
  10. Jeong H.J., Yoo Y.D., Kim J.S., Kim T.H., Kim J.H., Kang N.S. and Yih W. 2004. Mixotrophy in the phototrophic harmfulalga Cochlodinium polykrikoides (Dinophyceae): prey species, the effects of prey concentration, and grazing impact. J.Eukaryot. Microbiol. 51:563-569. https://doi.org/10.1111/j.1550-7408.2004.tb00292.x
  11. jeong H.J., Park J.Y., Nho, J.H., Park M.O., Ha J.H., Seong K.A., Jeng C., Seong C.N., Lee K.Y. and Yih W.H. 2005. Feedingby red-tide dinoflagellate on the cyanobacterium Synechococcus. Aquat. Microb. Ecol. 41:131-143. https://doi.org/10.3354/ame041131
  12. Lin S., Zhang H. and Dubois A. 2006. Low abundance distribution of Pfiesteria piscicida in Pacific and Western Atlantic asdetected by mtDNA-18S rDNA realtime polymerase chainreaction. J. Plankton Res. 28: 667-681. https://doi.org/10.1093/plankt/fbi150
  13. Liu K.K., Lai Z.L. and Gong G.C. 1995. Distribution of particulate organic matter in the southern East China Sea: implication in production and transport. Terr. Atmos. Ocean. Sci. 6:27-45.
  14. NFRDI. 2007. Data on red tides. http://www.nfrdi.re.kr/redtideInfo (written in Korean).
  15. Park T.G., de Salas M.F., Bolch C.J.S. and Hallegraeff G.M. 2007a. Development of a real-time PCR probe for quantification of the heterotrophic dinoflagellate Cryptoperidiniopsis brodyi (Dinophyceae) in environmental samples. Appl. Environ. Microbiol. 73:2552-2560. https://doi.org/10.1128/AEM.02389-06
  16. Park T.G., Bolch C.J.S. and Hallegraeff G.M. 2007b. Morphological and molecular genetic characterization of Cryptoperidiniopsis brodyi (Dinophyceae) from Australia-wide isolates. Harmful Algae 6: 718-733. https://doi.org/10.1016/j.hal.2007.02.004
  17. Park T.G., Park Y.T. and Lee Y. 2009a. Development of a SYT09based real-time PCR probe for detection and quantification of toxic dinoflagellate Karlodinium veneficum (Dinophyceae)in environmental samples. Phycolgia 48:32-43. https://doi.org/10.2216/08-52.1
  18. Park T.G., Bae H.M. and Lee Y. 2009b. Detection and quantification of fish killing dinoflagellate Karlodinium veneficum (Dinophyceae) in sediments by real-time PCR. Harmful Algae (In press).
  19. Park T.G., Park G.H., Park Y.T., Kang Y.S., Bae H.M., Kim C.H., Jeong H.J. and Lee Y. 2009c. Identification of the dinoflagellate community during Cochlodinium polykrikoides (Dinophyceae) blooms using amplified rDNA melting curve analysis and real-time PCR probes. Harmful Algae 8:430-440. https://doi.org/10.1016/j.hal.2008.09.003
  20. Place A.R., Saito K., Deeds J.R., Robledo j.A.F. and Vasta G.R. 2008. A decade of research on Pfiesteria spp. and their toxins: unresolved questions and an alternative hypothesis. In:Botana L.M. (ed.), Seafood and freshwatcr toxins. CRC Press,New York. pp. 717-751.
  21. Yoon Y.H., Park J.S., Seo H.Y. and Hwang D.J, 2003. Spatial distribution of phytoplankton community and red tide of dinoflagellate, Prorocentrum donghaience in the East China Sea during early summer. Kor. Soc. Environ. Bioi. 21: 132-141.

Cited by

  1. Development of real-time RT-PCR for detecting viable Cochlodinium polykrikoides (Dinophyceae) cysts in sediment vol.60, 2016, https://doi.org/10.1016/j.hal.2016.10.005
  2. Newly recorded Karlodinium veneficum dinoflagellate blooms in stratified water of the East China Sea vol.101, 2014, https://doi.org/10.1016/j.dsr2.2013.01.015