Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Direct Determination of Uric Acid in Human Serum Samples Using Polypyrrole Nanoelectrode Ensembles

  • Yang, Guangming (College of Chemistry and Chemical Engineering, Yunnan Normal University) ;
  • Tan, Lin (College of Chemistry and Chemical Engineering, Yunnan Normal University) ;
  • Shi, Ya (College of Chemistry and Chemical Engineering, Yunnan Normal University) ;
  • Wang, Suiping (State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University) ;
  • Lu, Xuxiao (College of Chemistry and Chemical Engineering, Yunnan Normal University) ;
  • Bai, Huiping (College of Chemistry and Chemical Engineering, Yunnan Normal University) ;
  • Yang, Yunhui (College of Chemistry and Chemical Engineering, Yunnan Normal University)
  • Published : 2009.02.20
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Abstract

Polypyrrole (PPy) nanotubes have been synthesized by chemical oxidative polymerization of pyrrole within the pores of polycarbonate membrane using the technology of diffusion of solutes. The nanotubes array prepared by the proposed method can be considered as nanoelectrode ensembles (NEEs). An amperometric uric acid sensor based on PPy NEEs has been developed and used for determination of uric acid in human serum samples. The electrode can direct response to uric acid at potential of 0.60V vs. SCE with wide linear range of $1.52{\times}10^{-6}\;to\;1.54{\times}10^{-3}\;M.\;The\;detection\;limit\;is \;3.02{\times}10^{-7}$ M. This sensor has been used to determine uric acid in real serum samples. PPy NEEs is thought of as a good application in the foreground.

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