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Effects of Overexpression of C5 Protein on rnpB Gene Expression in Escherichia coli

  • Published : 2009.04.20

Abstract

Escherichia coli RNase P is composed of a large RNA subunit (M1 RNA) and a small protein subunit (C5 protein). Since both subunits are assembled in a 1:1 ratio, expression of M1 RNA and C5 protein should be coordinately regulated for RNase P to be efficiently synthesized in the cell. However, it is not known yet how the coordination occurs. In this study, we investigated how overexpression of C5 protein affects expression of the rnpB gene encoding M1 RNA, using a lysogenic strain, which carries an rnpB-lacZ transcription fusion. Primer extension analysis of rnpB-lacZ fusion transcripts showed that the overexpression of C5 protein increased the amount of the fusion transcripts, suggesting that rnpB expression increases with the increase of intracellular level of C5 protein.

Keywords

References

  1. Robertson, H. D.; Altman, S.; Smith, J. D. J. Biol. Chem. 1972, 247, 5243.
  2. Bothwell, A. L.; Garber, R. L.; Altman, S. J. Biol. Chem. 1976, 251, 7709.
  3. Bourgaize, D.B. Nature 1987, 325, 281. https://doi.org/10.1038/325281a0
  4. Komine, Y.; Kitabatake, M.; Yokogawa, T.; Nishikawa, K.; Inokuchi, H. Proc. Natl. Acad. Sci. USA 1994, 91, 9223. https://doi.org/10.1073/pnas.91.20.9223
  5. Li, Y.; Altman, S. Proc. Natl. Acad. Sci. USA 2003, 100, 13213. https://doi.org/10.1073/pnas.2235589100
  6. Lee, J.; Kim, Y.; Hong, S. K.; Lee, Y. Bull. Korean Chem. Soc. 2008, 29, 1137. https://doi.org/10.5012/bkcs.2008.29.6.1137
  7. Motamedi, H.; Lee, Y.; Schmidt, F. J. Proc. Natl. Acad. Sci. USA 1984, 81, 3959. https://doi.org/10.1073/pnas.81.13.3959
  8. Hansen, F. G.; Hansen, E. B.; Atlung, T. Gene 1985, 38, 85. https://doi.org/10.1016/0378-1119(85)90206-9
  9. Guerrier-Takada, C.; Gardiner, K.; Marsh, T.; Pace, N.; Altman, S. Cell 1983, 35, 849. https://doi.org/10.1016/0092-8674(83)90117-4
  10. Sakano, H.; Yamada, S.; Ikemura, T.; Shimura, Y.; Ozeki, H. Nucleic Acids Res. 1974, 1, 355. https://doi.org/10.1093/nar/1.3.355
  11. Lumelsky, N.; Altman, S. J. Mol. Biol. 1988, 202, 443. https://doi.org/10.1016/0022-2836(88)90277-X
  12. Buck, A. H.; Dalby, A. B.; Poole, A. W.; Kazantsev, A. V.; Pace, N. R. EMBO J. 2005, 24, 3360. https://doi.org/10.1038/sj.emboj.7600805
  13. Kirsebom, L. A.; Altman, S. J. Mol. Biol. 1989, 207, 837. https://doi.org/10.1016/0022-2836(89)90250-7
  14. Kirsebom, L. A.; Svard, S. G. Nucleic Acids Res. 1992, 20, 425. https://doi.org/10.1093/nar/20.3.425
  15. Peck-Miller, K. A.; Altman, S. J. Mol. Biol. 1991, 221, 1.
  16. Hsieh, J.; Andrews, A. J.; Fierke, C. A. Biopolymers 2004, 73, 79. https://doi.org/10.1002/bip.10521
  17. Sun, L.; Campbell, F. E.; Zahler, N. H.; Harris, M. E. EMBO J. 2006, 25, 3998. https://doi.org/10.1038/sj.emboj.7601290
  18. Kim, Y.; Lee, Y. FEBS Lett. 2009, 583, 419. https://doi.org/10.1016/j.febslet.2008.12.040
  19. Jeon, E.; Lee H.; Lee S.; Jung,Y. H.; Cho, M.; Chung, J. H.; Lee, Y.; Park, C. Korean J. Genetics 1993, 15, 11.
  20. Park, B. H.; Choi, Y. N.; Park, J. W.; Sim, S.; Gil, M. C.; Kim, S.; Kim, M.; Lee, Y. Mol. Cells 1998, 8, 96.
  21. Kim, Y.; Han, K.; Lee, J.; Kim, K.; Lee, Y. Bull. Korean Chem. Soc.2007, 28, 1010. https://doi.org/10.5012/bkcs.2007.28.6.1010
  22. Bernstein, J. A.; Lin, P. H.; Cohen, S. N.; Lin-Chao, S. Proc. Natl. Acad. Sci. USA 2004, 101, 2758. https://doi.org/10.1073/pnas.0308747101

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