DOI QR코드

DOI QR Code

유자와 탱자 과피 에탄올 추출물의 MCF-7 유방암 세포에 대한 항암 활성

Anticancer Activity of Ethanol Extract from Peel of Citrus junos and Poncirus trifoliata on MCF-7 Breast Cancer Cells

  • 김지은 (동아대학교 생명공학과) ;
  • 박준희 (동의대학교 분자생물학과) ;
  • 강병원 (동아대학교 BK21 실버바이오 사업단) ;
  • 서민정 (동아대학교 생명공학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 임학섭 ((주)천년약속 바이오연구소) ;
  • 서권일 (순천대학교 식품영양학과) ;
  • 김정인 (인제대학교 식품생명과학부) ;
  • 주우홍 (창원대학교 생물학과) ;
  • 이복규 (동의대학교 분자생물학과) ;
  • 정영기 (동아대학교 생명공학과)
  • Kim, Ji-Eun (Department of Biotechnology, Dong-A University) ;
  • Park, Joon-Hee (Department of Molecular Biology, Dong-Eui University) ;
  • Kang, Byoung-Won (BK21 Center for Silver-Bio industrialization Project, Dong-A University) ;
  • Seo, Min-Jeong (Department of Biotechnology, Dong-A University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University) ;
  • Lim, Hak-Seob (Department Bioinstitute, MILLENNIUM PROMISE CO., LTD.) ;
  • Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University) ;
  • Kim, Jeong-In (School of Food and Life Science, Inje University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University) ;
  • Lee, Bok-Kyu (Department of Molecular Biology, Dong-Eui University) ;
  • Jeong, Yong-Kee (Department of Biotechnology, Dong-A University)
  • 발행 : 2008.10.30

초록

본 연구는 MCF-7 유방암 세포에 대한 유자(CJP)와 탱자 (PTP) 과피 추출물의 항암 활성과 환경호르몬에 의해 유도된 암세포의 증식 억제 효과에 대하여 조사하였다. CJP와 PTP를 300 mg/ml 농도에서 72시간 처리하였을 경우, 암세포의 성장을 저해하였고 세포사멸을 유도하였다. MCF-7 유방암 세포의 형태학적 변화는 CJP와 PTP를 500 mg/ml 농도에서 7시간 처리하였을 경우 관측되었고 세포사멸은 capase-3의 활성화에 의하여 유도되었다. 환경호르몬에 의해 유도된 MCF-7 유방암 세포의 증식은 CJP와 PTP의 처리로 인하여 농도 의존적으로 감소하였으며, 300 mg/ml 농도에서는 대조군과 비교하였을 때 각각 70%와 80% 이상 감소하였다.

In this study, anti-cancer activities of peel of Citrus junos (CJP) and Poncirus trifoliata (PTP) on MCF-7 breast cancer cells, and anti-proliferative effects of cancer cells induced by environmental hormones were investigated. The ethanol extracts of CJP and PTP inhibited cancer cell growth and induced apoptosis at the concentration over 300 mg/ml treatment for 72 hr. Morphological change of MCF-7 breast cancer cells were observed treated with the CJP and PTP of 500 mg/ml concentration for 72 hr, and apoptosis was induced by activation of caspase-3. The proliferation of MCF-7 breast cancer cells was decreased in a dose-dependent manner treated with various concentration of CJP and PTP, when compared with the control at 300 mg/ml, the proliferation of the MCF-7 breast cancer cells of both extracts was decreased over 70% and 80%, respectively.

키워드

참고문헌

  1. Aobchey, P., S. Sinchaikul, S. Phutrakul and S. T. Chen. 2007. Simple purification of indirubin from Indigofera tinctoria Linn. and inhibitory effect on MCF-7 human breast cancer cells. Chiang Mai Journal of Science 34, 329-337.
  2. Chen, W. F., M. H. Huang, C. H. Tzang, M. Yang and M. S. Wong. 2003. Inhibitory actions of genistein in human breast cancer (MCF-7) cells. Biochimica et Biophysica Acta. Molecular Basis of Disease 1638, 187-196. https://doi.org/10.1016/S0925-4439(03)00082-6
  3. Choi, Y. H., J. H. Baek, M. A. Yoo, H. Y. Chung and K. W. Kim. 2000. Induction of apoptosis by ursolic acid through activation of caspases and down-regulation of c-IAPs in human prostate epithelial cells. Int. J. Oncol. 17, 971-976.
  4. Choi, Y. H., H. S. Eo, Y. G. Jin, Y. H. Yoo and N. Kim. 2003. Apoptosis and modulation of cell control by synthetic derivatives of ursodeoxycholic acid and chenodeoxycholic acid in human prostate cancer cells. Cancer Letters 199, 157-167. https://doi.org/10.1016/S0304-3835(03)00351-3
  5. Chung, H. S., J. B. Lee, J. H. Seong and J. U. Choi. 2004. Chemical components in peel and flesh of trifoliata oranges (Poncirus trifoliata). Kor. J. Food Pre. 11, 342-346.
  6. Dong, S., A. Inoue, Y. Zyu, M. Tanji and R. Kiyama. 2007. Activation of rapid signaling pathways and the subsequent transcriptional regulation for the proliferation of breast cancer MCF-7 cells by the treatment with an extract of Glycyrrhiza glabra root. Food and Chemical Toxicology 45, 2470-2478. https://doi.org/10.1016/j.fct.2007.05.031
  7. Han, D. H., Y. Miyazaki and H. Tachibana. 2000. Optimization of estrogenic activity detection method using human breast cancer MCF-7 cells. J. Fac. Agr., Kyushm Univ. 44, 339-348.
  8. Hwang, Y. J., H. K. Nam, M. J. Chang, G. W. Noh and S. H. Kim. 2003. Effect of Lentinus edodes and Pleurotus eryngii. Extracts on proliferation and apoptosis in human colon cancer cell lines. J. Kor Soc. Food Sci. Nutr. 32, 217-222. https://doi.org/10.3746/jkfn.2003.32.2.217
  9. Jayaprakasha, G. K., K. Mandadi, K. Poulose, M. Shibu, Y. Jadegoud, G. Gowda, G. A. Nagana, G. Patil and S. Bhimanagouda. 2007. Inhibition of colon cancer cell growth and antioxidant activity of bioactive compounds from Poncirus trifoliata (L.) Raf. Bioorganic & Medicinal Chemistry 15, 4923-4932. https://doi.org/10.1016/j.bmc.2007.04.044
  10. Johnson, M. K. and G. Loo. 2000. Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA. Mutat. RES. 459, 211-218. https://doi.org/10.1016/S0921-8777(99)00074-9
  11. Kang, S. C., C. M. Lee, E. S. Choung, J. P. Bak, J. J. Bae, H. S. Yoo, J. H. Kwak and O. P. Zee. 2008. Anti-proliferative effects of estrogen receptor-modulating compounds isolated from Rheum palmatum. Archives of Pharmacal Research 31, 722-726. https://doi.org/10.1007/s12272-001-1218-1
  12. Kang, S. K., M. J. Jang and Y. D. Kim. 2006. A study on the flavor constituents of the Citron (Citrus junos). Kor. J. Food Preserv. 13, 204-210.
  13. Kawaii, S., Y. Tomono, E. Katase, K. Ogawa and M. Yano. 1999. Quantitation of flavonoid constituents in citrus fruits. J. Agric. Food Chem. 47, 3565-3571. https://doi.org/10.1021/jf990153+
  14. Kim, H. W., S. J. Cho, B. Y. Kim and S. I. Cho 2007. Anti-oxidant effects of Anemarrhenae Rhizoma in three different lineages. Kor. J. Orient. Int. Med. 28, 608-614.
  15. Kim, T. J., J. Y. No and J. S. Ko. 1989. The separation and determination of flavonoid glycosides from Poncirus trifoliata rafia and Citrus aurantium. L. J. Kor. Soc. Ana. Sci. 2, 302-307.
  16. Lee, Y. G. and I. H. Cha. 2001. Antibacterial activity of Poncirus trifoliata juice against pathogenic bacteria. Kor. J. Life Sci. 11, 534-560.
  17. Murakami, H. and T. Sugahara. 1994. Animal cell technology, pp. 215-233, Corona Publishing Co. Japan.
  18. Nakagawa, Y. and T. Suzuki. 2001. Metabolism for bisphenol A in isolated rat hepatocytes and estrogenic activity of a hydroxylated metabolite in MCF-7 human breast cancer cell. Xenobiotica 31, 113-123. https://doi.org/10.1080/00498250110040501
  19. Park, K. D., B. S. Han and K. H. Rhim. 2006. Modifying effect of bisphenol A on the preneoplastic and neoplastic lesion if rat liver treated with diethylnitrosamine infusion. Kor. J. Env. Hlth. 32, 60-66.
  20. Skehan, P. R., D. Stoveng, A. Scudiero, J. Monks, D. McMahon, J. T. Vistica, H. Warren, S. Bokesch, M. Kenney and R. Boyd. 1990. New colorimetric cytotoxicity assay for anticancer drug screening. T. Nat. Cancer Inst. 82, 1107-1112. https://doi.org/10.1093/jnci/82.13.1107
  21. Soung, M. G., S. M. Joo, A. R. Song and N. D. Sung. 2007. QSAR on the inhibition activity of flavopiridol analogues against breast cancer MCF-7. J. Korean Soc. Appl. Biol. Chem. 50, 147-153
  22. Steller, H. 1995. Mechanisms and genes of cellular suicide. Science 267, 1445-1449. https://doi.org/10.1126/science.7878463
  23. Yi, J. M., M. S. Kim, H. N. Koo, B. K. Song, H. Y. Yoo and H. M. Kim. 2004. Poncirus trifoliata fruit induces apoptosis in human promyelocytic leukemia cells. Clinica Chimica Acta. 340, 179-185. https://doi.org/10.1016/j.cccn.2003.10.017
  24. Yoo, K. M. and I. K. Hwang. 2004. In vitro Effect of Yuza (Citrus junos SIEB ex TANAKA) extracts on proliferation of human prostate cancer cells and antioxidant activity. Kor. J. Food Sci. Technol. 36, 339-344.

피인용 문헌

  1. Anticancer Activity of Methyl Gallate in RC-58T/h/SA#4 Primary Human Prostate Cancer Cells vol.43, pp.3, 2014, https://doi.org/10.3746/jkfn.2014.43.3.367
  2. Induction of Apoptosis in Human Cancer Cells with Extracts of Taraxacum coreanum, Youngia sonchifolia and Ixeris dentate vol.31, pp.1, 2016, https://doi.org/10.13103/JFHS.2016.31.1.51
  3. Antiproliferative and Apoptotic Effects of Sasa quelpaertensis Nakai in Human Cancer Cells vol.24, pp.8, 2014, https://doi.org/10.5352/JLS.2014.24.8.903